Cordyceps sinensis strain capable of producing sporocarp on artificial culture medium
A Cordyceps sinensis fungus and a technology for producing Cordyceps sinensis, applied in the field of Cordyceps sinensis strains
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Embodiment 1
[0032] Example 1. Isolation and purification of Cordyceps sinensis ZDG02.
[0033] The isolation method of the bacterial strain involved in the present invention is a tissue isolation method, and the experimental material is the fruiting body part of fresh Cordyceps sinensis produced in Daigan pasture in Tianzhu Tibetan Autonomous County, Wuwei, Gansu. The main steps are as follows: 1. Rinse intact fresh Cordyceps sinensis with distilled water to remove surface soil and dry the surface water with sterile filter paper. 2. Disinfect Cordyceps sinensis in 75% alcohol for 30 seconds, then rinse with sterile water three times. 3 Put Cordyceps sinensis into 0.1% mercuric chloride solution for surface disinfection for 2 minutes, rinse with sterile water 5 times, and dry the surface with sterile filter paper. 4. Cut the fruiting body part into 2mm small pieces with a sterile scalpel and inoculate them in milk culture medium at 18°C in the dark and dark. 5. When colonies visible to...
Embodiment 2
[0034] Example 2, the biological characteristics of Cordyceps sinensis ZDG02.
[0035] 1. Morphological characteristics: On the solid milk medium, the colonies are brownish-yellow as a whole, with a tough texture. The aerial hyphae are undeveloped at the initial stage, and dark brown pigments are secreted into the medium. With the extension of culture time, the colonies will appear irregular Wrinkled and uplifted as a whole, presenting the shape of vermicompost ( figure 1 ), when cultured to more than 90 days, obvious aerial hyphae began to appear ( figure 2 ), under the microscope, the aerial hyphae were transparent, with septum and kidney-shaped conidia, the conidia were colorless without septum, and the amount of spores was not much ( image 3 ).
[0036] 2. Molecular biological characteristics: The third method in the OMEGA kit was used to extract the total DNA of Cordyceps sinensis ZDG02, and the ITS4 / ITS5 universal primer was used to amplify the sequence of the riboso...
Embodiment 3
[0038] Example 3. Research on fruiting bodies produced by deep culture of Cordyceps sinensis fungus ZDG02.
[0039] 1. Inoculate the purified Cordyceps sinensis ZDG02 into a slant test tube with broth medium, plug the test tube tightly with a silica gel stopper and incubate at 18°C in the dark for 120 days.
[0040] 2. After step 1, transfer to a 15°C incubator for 100 days.
[0041] 3. After step 2, transfer to a 12°C incubator for 100 days.
[0042] 4. After step 3, transfer to 4°C for cultivation.
[0043] When the total culture time was close to 500 days, Cordyceps sinensis ZDG02 grew fruiting bodies consistent with natural Cordyceps on the broth medium, such as Figure 4 shown.
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Abstract
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