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IncRNA molecule and application thereof

A technology of molecules and kits, applied in the field of molecular biomedicine, can solve problems such as effects that have not been reported yet

Active Publication Date: 2021-05-28
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its role in AAV has not been reported yet

Method used

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  • IncRNA molecule and application thereof
  • IncRNA molecule and application thereof
  • IncRNA molecule and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening of AAV-related lncRNA molecules:

[0042] Venous blood was collected from AAV patients and healthy controls (5 cases in each group), peripheral blood leukocytes were separated, total RNA was extracted, and differentially expressed lncRNAs were screened out using RNA-seq technology (see figure 1 ).

Embodiment 2

[0044] Differentially expressed genes screened out by large sample verification:

[0045] 1. Sample collection

[0046] A total of 35 patients who were diagnosed with GPA and MPA according to the diagnostic criteria of ACR in 1990 and CHCC in 2012 in the Department of Rheumatology and Immunology, Zhongshan Hospital Affiliated to Fudan University from January 2019 to December 2020 were collected, and patients with other autoimmune diseases, tumors and infections were excluded. At the same time, 20 healthy subjects were collected as controls. 10ml of venous blood was collected from patients and healthy controls with EDTA blood collection tubes. Informed consent of patients and healthy controls was obtained for specimen collection in the experiment.

[0047] 2. Extraction of total RNA from samples

[0048]1) Add the collected venous blood to the erythrocyte lysate at a ratio of 20:1, let it stand at room temperature for 5-7min, centrifuge at 400g for 10min after the erythrocyt...

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Abstract

The invention relates to an lncRNA molecule and application thereof, and belongs to the technical field of molecular biomedicine. The invention provides an lncRNA molecule with high expression in peripheral blood of an AAV patient, and the lncRNA molecule is named as LINC0219. High-expression lncRNA in peripheral blood of an AAV patient is successfully screened through RNA-seq and clinical large sample verification, and the lncRNA can be used as a molecular marker or a target for AAV diagnosis or evaluation, is applied to early diagnosis and activity evaluation of AAV or can be used as a treatment target, can be used for further illuminating a molecular mechanism, a signal channel and the like of AAV morbidity, and has a great application prospect.

Description

technical field [0001] The invention relates to an lncRNA molecule and its application, belonging to the technical field of molecular biomedicine. Background technique [0002] Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of multisystem autoimmune inflammatory diseases characterized by necrotizing vasculitis of small and medium vessels. AAV includes three distinct clinical types: granulomatous polyangiitis (GPA), microscopic polyangiitis (MPA), and eosinophilic granulomatous polyangiitis (EGPA). The disease can affect almost any system organ. The clinical manifestations are non-specific and the disease progresses rapidly. If early diagnosis and timely treatment are not available, the lungs, kidneys, digestive tract and other important organs are often involved, causing irreversible organ damage or even life-threatening. . Moreover, the repeated recurrence of the disease has led to difficulties in treatment. If the disease activity canno...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/6851C12Q1/6883
CPCC12N15/113C12Q1/6851C12Q1/6883C12N2310/11C12Q2600/158C12Q2600/178C12Q2531/113C12Q2563/107C12Q2545/101
Inventor 姜林娣刘云
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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