Composition with anti-aging and moisturizing effects as well as preparation method and application thereof

A composition and anti-aging technology, applied in medical preparations containing active ingredients, skin care preparations, pharmaceutical formulations, etc.

Inactive Publication Date: 2021-06-01
SHANGHAI PECHOIN BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of anti-aging active substances effectively entering the skin while reducing irritation and dry lines in the prior art, the present invention proposes an anti-aging and moisturizing composition comprising different proportions of hyaluronic acid microcapsules and active substance lipids Body, while reducing irritation, improve the skin's absorption of the composition, supplement the skin with sodium hyaluronate and promote collagen production, repair damaged skin barrier, reduce dry lines and fine lines, achieve anti-aging moisturizing the goal of

Method used

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  • Composition with anti-aging and moisturizing effects as well as preparation method and application thereof
  • Composition with anti-aging and moisturizing effects as well as preparation method and application thereof
  • Composition with anti-aging and moisturizing effects as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] A preparation method of an anti-aging moisturizing composition is as follows:

[0037] 1) Add 3 parts of glycerin and 1 part of water to the active liposome in proportion, homogenize for 2 minutes, and homogenize at a speed of 6000-8000 rpm, keep warm for later use;

[0038] 2) Add 0.08 parts of sodium acrylate / sodium acryloyldimethyltaurate copolymer and 4 parts of water to the hyaluronic acid microcapsules in proportion, mix well and set aside;

[0039] 3) Slowly add the material obtained in 1) into the material obtained in 2) while stirring, mix evenly, homogenize the obtained material for 1 minute with a high-pressure homogenizer, and filter with a 1 μm filter to obtain a composition, all in parts by weight.

[0040] The preparation of the hyaluronic acid microcapsules comprises the following steps:

[0041] 1) Add 0.6 parts of hyaluronic acid derivatives to 25 parts of water and stir evenly to obtain the first component, keep it warm for later use;

[0042] 2) Ad...

experiment example 1

[0058] Experimental example 1. Cytotoxicity evaluation test: to detect the safety of the composition

[0059] MTT assay is an experimental method widely used in the detection of cytotoxicity or cell proliferation by measuring the number of living cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can make the water-soluble yellow salt MTT (3- (4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) is reduced to water-insoluble blue formazan and deposited in cells, while dead cells have no such function. The generated crystalline formazan is generally added to DMSO (dimethyl sulfoxide), and the absorbance is measured after dissolution. The specific experimental method is in a 96-well plate according to 1X10 4 Inoculate 100 μl of DMEM medium containing 10% bovine serum and 100 μl of keratinocytes (HaCaT) at a density of one per well, and replace with serum-free medium after 24 hours of culture. Different concentrations of the c...

experiment example 2

[0066] Experimental Example 2. Hyaluronidase Inhibition Experiment

[0067] Compositions 1-7 and Comparative Examples 1-3 were formulated into 1% aqueous solutions as sample liquids. Add 0.1mL 0.25mmol / L CaCl to the test tube 2 Solution and 0.5mL hyaluronidase solution, incubated at 37°C for 20min; added 0.5mL sample solution, incubated at 37°C for 20min; added 0.5mL sodium hyaluronate solution, incubated at 37°C for 30min; mol / L NAOH solution 0.1ml and acetylacetone solution 0.5ml, heat reaction in boiling water bath for 15min, then ice-bath for 5min; then add 1.0mL Ehrlich reagent and dilute with 3.0mL absolute ethanol, stand for 20min for color development , measure its absorbance value, and substitute it into the following formula to calculate the inhibition rate.

[0068] Hyaluronidase inhibition rate (%)=[(A-B)-(C-D)] / (A-B)*100%

[0069] In the formula: A is the absorbance value of the control solution (replace the sample solution with acetic acid buffer solution); B ...

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Abstract

The invention provides a composition with anti-aging and moisturizing effects as well as a preparation method and application thereof. The composition is composed of hyaluronic acid microcapsules and active matter lipidosome, active matter is wrapped with the microcapsules and the lipidosome, the active matter is slowly released, the availability of the active matter is improved, and meanwhile the stability of the microcapsules and the lipidosome is improved. By adjusting the proportion of the hyaluronic acid microcapsules to the active matter liposome, better anti-aging and moisturizing effects are achieved. Through the microcapsules and the lipidosome, the composition has better effects of moisturizing the surface layer, deeply supplementing sodium hyaluronate and promoting the synthesis of collagen, and finally achieves the purposes of resisting aging and moisturizing.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to a composition with anti-aging and moisturizing effects, a preparation method and application thereof. Background technique [0002] With the development of human society and the acceleration of population aging, the problem of aging has become a concern of the whole society. With the growth of age, the morphological structure and physiological function of the skin change. Skin aging is the result of multiple factors. Stress, strain, injury, infection, malnutrition, metabolic disorders, and drug abuse will all affect the skin condition. Healthy skin should be smooth, moist and elastic. Free radicals destroy collagen and active substances in normal cell membranes to form small fine lines and wrinkles. Skin loses elasticity due to evaporation and dehydration, and dry lines will form. [0003] Currently commercially available anti-aging products mainly use some active substance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/73A61K8/11A61K8/14A61K8/60A61K8/64A61K8/67A61Q19/00A61Q19/08
CPCA61K8/735A61K8/64A61K8/676A61K8/602A61K8/11A61K8/14A61Q19/00A61Q19/08A61K2800/56A61K2800/82
Inventor 孟庆洋李基西林贞翔罗丹周秋娜胡霞云金荣熙金延埈
Owner SHANGHAI PECHOIN BIOTECH CO LTD
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