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Method for enriching and purifying cyclopeptide Xylastriamide A from xylaria longitudinal stripe

A technology of longitudinal stripes and charcoal bacteria, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of adsorption-desorption methods that have not yet been seen, and achieve strong practicability, improved purity of components, and fewer separation steps Effect

Active Publication Date: 2021-06-01
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no literature report on the adsorption-desorption method of the cyclic peptide Xylastriamide A in C. vertical stripes on the macroporous adsorption resin

Method used

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  • Method for enriching and purifying cyclopeptide Xylastriamide A from xylaria longitudinal stripe
  • Method for enriching and purifying cyclopeptide Xylastriamide A from xylaria longitudinal stripe
  • Method for enriching and purifying cyclopeptide Xylastriamide A from xylaria longitudinal stripe

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:

[0038] a. Prepare the sample solution of Xylella vertical stripes:

[0039] Get 100g of the fruiting bodies of Xanthomonas vertical stripes (from Microbiology Laboratory of Southwest University of Science and Technology, the same afterward), pass through a 40-mesh sieve after crushing; add 20mL volume percentage concentration of 90% ethanol aqueous solution according to the ratio of 1g of Xanthomonas vertical stripes fruiting bodies, Mix the fruiting bodies of Xanthocystis verticalis and 90% ethanol water solution by volume, soak at 80°C for 2 hours, filter, and then process the solid matter twice through the soaking process, and combine the filtrate; the filtrate is concentrated to an extract by rotary evaporation Finally, add the extract to distilled water to dissolve, and prepare a solution with a concentration of 0.0030g / mL, which is the prepa...

Embodiment 2

[0046] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:

[0047] a. Prepare the sample solution of Xylella vertical stripes:

[0048] Get 100g of the fruiting bodies of Xylocarpella vertically striped, pass through a 40 mesh sieve after crushing; add 20mL of 80% ethanol aqueous solution according to the ratio of 1g of the fruiting bodies of Xylocarpyr vertically stripes and the concentration of 80% ethanol by volume. % ethanol aqueous solution was mixed and soaked at 85°C for 2 hours, filtered, the solid matter was treated twice through the soaking process, and the filtrate was combined; the filtrate was concentrated to the extract by rotary evaporation, and then the extract was dissolved in distilled water to prepare The solution with a concentration of 0.0050g / mL is the prepared sample solution of Xylocarpus longitudinally stripes, which is set aside;

[0049] b. Kinetic adsorption of the resin on the...

Embodiment 3

[0055] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:

[0056] a. Prepare the sample solution of Xylella vertical stripes:

[0057] Get 100g of the fruiting bodies of Pyrocarpyra verticalis, and pass through a 40-mesh sieve after being pulverized; add 20mL of 85% ethanol aqueous solution according to the ratio of 1g of Pyrethymus vertically striped fruiting bodies and the concentration of 85% ethanol by volume. % ethanol aqueous solution was mixed and soaked at 70°C for 2 hours, filtered, and the solid matter was treated by the soaking process for 3 times, and the filtrate was combined; the filtrate was concentrated to the extract by rotary evaporation, and then the extract was dissolved in distilled water to prepare The solution with a concentration of 0.0010g / mL is the prepared sample solution for Xylocarpus longitudinally stripes, which is set aside;

[0058] b. Kinetic adsorption of the resin on t...

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Abstract

The invention discloses a method for enriching and purifying cyclopeptide Xylastriamide A from xylariastriata. The method is characterized by comprising the following steps: preparing a xylariastriata loading solution; pretreating D101 resin, packing the pretreated D101 resin into a column, and adding a loading solution for dynamic adsorption; and carrying out dynamic desorption on the cyclic peptide Xylasticamide A in the xylariastriata by using the resin. By adopting the method disclosed by the invention, the purity of the component of the cyclopeptide XylastriamideA purified by the D101 resin is greatly improved, the enrichment and purification effects are obvious, the operation is simple and convenient, the separation steps are few, the dosage of an organic solvent is small, the time is short, and the enriched and purified cyclopeptide XylastriamideA has obvious anti-tumor and nematicidal activity and is applicable to related medicines.

Description

technical field [0001] The invention belongs to the separation and purification of organic compounds, and relates to a method for enriching and purifying the cyclic peptide Xylastramide A from C. It is used in medicine as an active ingredient of traditional Chinese medicine. Background technique [0002] Xylaria striata Pat.1887 is a fungus of the genus Xylaria Hill ex Schrank in the family Xylariaceae of the phylum Ascomycota, and its growth range is mainly on decaying bark and living tree roots. The fungus of the genus Pyramidus has great research, medicinal, and edible value. For example, Pyrethymus nigriscens (commonly known as Wulingshen) has the functions of dehumidification, calming the nerves, hematopoiesis, and improving the immune function of the body. Its health-preserving effect is excellent. As a fungus discovered by the respondent, Xylella vertical stripes has been studied on the safety of the fungus and the improvement of sleep, and the culture conditions of ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/22
CPCC07K7/06
Inventor 任志洋袁小红
Owner SOUTHWEAT UNIV OF SCI & TECH
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