Method for enriching and purifying cyclopeptide Xylastriamide A from xylaria longitudinal stripe
A technology of longitudinal stripes and charcoal bacteria, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of adsorption-desorption methods that have not yet been seen, and achieve strong practicability, improved purity of components, and fewer separation steps Effect
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Embodiment 1
[0037] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:
[0038] a. Prepare the sample solution of Xylella vertical stripes:
[0039] Get 100g of the fruiting bodies of Xanthomonas vertical stripes (from Microbiology Laboratory of Southwest University of Science and Technology, the same afterward), pass through a 40-mesh sieve after crushing; add 20mL volume percentage concentration of 90% ethanol aqueous solution according to the ratio of 1g of Xanthomonas vertical stripes fruiting bodies, Mix the fruiting bodies of Xanthocystis verticalis and 90% ethanol water solution by volume, soak at 80°C for 2 hours, filter, and then process the solid matter twice through the soaking process, and combine the filtrate; the filtrate is concentrated to an extract by rotary evaporation Finally, add the extract to distilled water to dissolve, and prepare a solution with a concentration of 0.0030g / mL, which is the prepa...
Embodiment 2
[0046] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:
[0047] a. Prepare the sample solution of Xylella vertical stripes:
[0048] Get 100g of the fruiting bodies of Xylocarpella vertically striped, pass through a 40 mesh sieve after crushing; add 20mL of 80% ethanol aqueous solution according to the ratio of 1g of the fruiting bodies of Xylocarpyr vertically stripes and the concentration of 80% ethanol by volume. % ethanol aqueous solution was mixed and soaked at 85°C for 2 hours, filtered, the solid matter was treated twice through the soaking process, and the filtrate was combined; the filtrate was concentrated to the extract by rotary evaporation, and then the extract was dissolved in distilled water to prepare The solution with a concentration of 0.0050g / mL is the prepared sample solution of Xylocarpus longitudinally stripes, which is set aside;
[0049] b. Kinetic adsorption of the resin on the...
Embodiment 3
[0055] A method for enriching and purifying the cyclic peptide Xylastramide A from Pyrethia spp., comprising the following steps:
[0056] a. Prepare the sample solution of Xylella vertical stripes:
[0057] Get 100g of the fruiting bodies of Pyrocarpyra verticalis, and pass through a 40-mesh sieve after being pulverized; add 20mL of 85% ethanol aqueous solution according to the ratio of 1g of Pyrethymus vertically striped fruiting bodies and the concentration of 85% ethanol by volume. % ethanol aqueous solution was mixed and soaked at 70°C for 2 hours, filtered, and the solid matter was treated by the soaking process for 3 times, and the filtrate was combined; the filtrate was concentrated to the extract by rotary evaporation, and then the extract was dissolved in distilled water to prepare The solution with a concentration of 0.0010g / mL is the prepared sample solution for Xylocarpus longitudinally stripes, which is set aside;
[0058] b. Kinetic adsorption of the resin on t...
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