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Application of reagent for detecting SNF5 gene expression in preparation of atherosclerosis diagnosis product

A technology of atherosclerosis and gene expression, applied in the medical field, can solve the problem that the role of SNF5 in atherosclerosis has not been elucidated

Pending Publication Date: 2021-06-01
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the role of SNF5 in the pathogenesis of atherosclerosis has not yet been elucidated.

Method used

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  • Application of reagent for detecting SNF5 gene expression in preparation of atherosclerosis diagnosis product
  • Application of reagent for detecting SNF5 gene expression in preparation of atherosclerosis diagnosis product
  • Application of reagent for detecting SNF5 gene expression in preparation of atherosclerosis diagnosis product

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0026] The Gene ID of the mouse SNF5 gene involved in the following technical scheme: 6598, the Gene ID of the human SNF5 gene: 20587.

[0027] Concrete technical scheme of the present invention is:

[0028] (1) Establish ApoE - / - Mouse model of atherosclerosis: ApoE - / - Mice were randomly divided into model control group (ApoE - / - Mice+common feed, A-control group, n=10) and hyperhomocysteinemia group (ApoE - / - mice+high methionine diet, HHcy group, n=10); and a normal control group (C57BL / 6J mice...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to application of a reagent for detecting SNF5 gene expression in preparation of an atherosclerosis diagnosis product. According to the application, the expression level of SNF5 in the process of promoting atherosclerosis inflammation by Hcy is detected, SNF5 overexpression lentivirus and SNF5 small RNA interference fragments are synthesized in vitro, the influence of SNF5 overexpression lentivirus and SNF5 small RNA interference fragments on IL-1beta in the atherosclerosis process is researched, and the effect of SNF5 in the process of promoting atherosclerosis inflammation by Hcy is clarified. The SNF5 involved in the invention plays an important role in promoting atherosclerosis inflammation by Hcy, provides a theoretical basis for diagnosing atherosclerosis from a molecular level in the future, and has great theoretical significance and potential practical values. The method is simple and easy to implement and easy to popularize.

Description

technical field [0001] The invention relates to the field of medical technology, and in particular to the application of a reagent for detecting SNF5 gene expression in the preparation of atherosclerosis diagnostic products. Background technique [0002] Atherosclerosis (atherosclerosis) is a chronic systemic disease involving multiple cells, multiple factors, and multiple links caused by injury or biochemical stimuli acting on the arterial wall. Mass accumulation, extracellular matrix protein deposition, arterial intima calcification, resulting in decreased arterial elasticity, stenosis of the lumen, may also lead to the formation or even rupture of arterial thrombus, seriously endangering human health. To sum up, researchers' views on the pathogenesis of atherosclerosis mainly include the theory of inflammatory response, the theory of smooth muscle clone proliferation, the theory of vascular injury response, the theory of thrombosis mosaicism and other theories. At presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61P9/10
CPCC12Q1/6883G01N33/6893A61K45/00A61P9/10C12Q2600/136C12Q2600/158G01N2800/324
Inventor 姜怡邓张慧萍谢琳马胜超熊建团张宏红盛思琪马飞揭育祯
Owner NINGXIA MEDICAL UNIV
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