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High-flux micro-fluidic chip and preparation method thereof

A microfluidic chip, high-throughput technology, applied in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve problems such as the inability to meet the requirements of throughput, and achieve the effect of simple operation

Inactive Publication Date: 2021-06-11
陕西融光云生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the reported highest throughput of the current valve-controlled microfluidic chip is only 1500, which is far from meeting the throughput requirements for screening combination therapy drugs and complex drug condition experiments.

Method used

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  • High-flux micro-fluidic chip and preparation method thereof
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  • High-flux micro-fluidic chip and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0049] See attached Figure 1-2 As shown, the embodiment of the present invention discloses a high-throughput microfluidic chip, including a first chip layer 1, a second chip layer 2, a third chip layer 3 and a bottom plate 4 connected in sequence;

[0050] The first chip layer 1 includes a first fluid layer 11 and a first control layer 12 connected in sequence, the second chip layer 2 includes a second fluid layer 21 and a second control layer 22 connected in sequence, and the third chip layer 3 includes a layer connected in sequence. The third fluid layer 31 and the third control layer 32;

[0051] The first control layer 12, the second fluid layer 21, the second control layer 22, the third fluid layer 31, the third control layer 32 and the bottom plate 4 are sequentially connected;

[0052] The first control layer 12 , the second control layer 22 and the third control layer 32 are sequentially connected through control channels.

[0053]Specifically, the first fluid layer...

Embodiment 2

[0084] Embodiment 2 of the present invention provides a method for preparing a high-throughput microfluidic chip, including:

[0085] S1: Place the mask on the silicon wafer spin-coated with SU-8 photoresist, and sequentially expose, develop and bake the silicon wafer to obtain the first fluid layer 11, the second fluid layer 21, and the third fluid layer. The first template, the second template, the third template, the fourth template, the fifth template and the sixth template corresponding to the layer 31, the first control layer 12, the second control layer 22 and the third control layer 32 in sequence;

[0086] Wherein, when preparing the first template, the second template, and the third template, the exposure time can be 10-20 seconds, pause for 30 seconds, and then continue to expose for 4-8 times, wherein 20 seconds for each exposure and 5 exposures are the best. When the structural resolution is the highest and the adhesion between the photoresist and the silicon wafe...

Embodiment 3

[0103] Applying the chip disclosed in Example 1 of the present invention to drug screening, this chip can perform ultra-high-throughput screening of various drug combinations and time series, and simultaneously construct in vitro cell and organoid models of various diseases. The in vivo microenvironment is simulated, and different drug combination sequences and in vitro cell models will produce different response effects. The specific process is as follows:

[0104] 1. Connect the chip according to the test steps, and complete operations such as turning on the gas-driven pump, checking the control valve, and adjusting the control pressure.

[0105] 2. Digest and dissociate the mouse primary neural stem cells that have been prepared in advance, including extraction, cryopreservation, recovery and passage proliferation, and place them in a centrifuge tube connected to the chip.

[0106] 3. Pressurize the centrifuge tube by 2-5psi, open the control valve connected to the chip in...

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Abstract

The invention discloses a high-flux micro-fluidic chip and a preparation method thereof. The chip structurally comprises a first chip layer, a second chip layer, a third chip layer and a bottom plate which are connected in sequence, the first chip layer comprises a first fluid layer and a first control layer which are connected in sequence, the second chip layer comprises a second fluid layer and a second control layer which are connected in sequence, and the third chip layer comprises a third fluid layer and a third control layer which are connected in sequence; the first control layer, the second fluid layer, the second control layer, the third fluid layer, the third control layer and the bottom plate are connected in sequence; and the three fluid layers are of a staggered layer structure, and 19800 closed in-vitro microenvironments capable of being accurately controlled are constructed in the chip while mutual interference of fluorescence signals of all the layers is avoided.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, and more specifically relates to a high-throughput microfluidic chip and a preparation method thereof. Background technique [0002] At present, the microfluidic chip is a technology that processes pipelines and reaction chambers on the chip, and drives reagents to flow in the pipelines and chambers to complete various biological and chemical processes. In recent years, microfluidic chips have begun to develop rapidly in the direction of functionalization and integration, and important biological and chemical processes such as nucleic acid amplification reactions and immune reactions have become new hotspots. [0003] However, the reported highest throughput of the current valve-controlled microfluidic chip is only 1500, which is far from meeting the throughput requirements for screening combination therapy drugs and complex drug condition experiments. [0004] Therefore, how to provi...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L3/50273B01L2300/0887B01L2300/0819
Inventor 张策张琛孙聃董宇峥李凯
Owner 陕西融光云生物科技有限公司
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