Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Muskmelon downy mildew resistance InDel marker and application thereof

A technology for downy mildew resistance and markers, applied in the field of melon downy mildew resistance molecular markers and its application, molecular genetics and breeding, can solve the time-consuming problem of undisclosed melon downy mildew resistance InDel molecular markers and disease-resistant materials , easily affected by the environment and other issues, to achieve the effect of improving breeding efficiency, high accuracy, and shortening the breeding cycle

Pending Publication Date: 2021-06-11
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of traditional methods such as time-consuming, labor-intensive, and susceptible to environmental influences in breeding disease-resistant materials, the prior art has not disclosed the InDel molecular marker for melon resistance to downy mildew

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Muskmelon downy mildew resistance InDel marker and application thereof
  • Muskmelon downy mildew resistance InDel marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: InDel Molecular Marker of Melon Downy Mildew Resistance

[0049] The invention discloses downy mildew resistant InDel molecular markers of muskmelon, and the InDel molecular markers are InDel 15 and InDel 20.

[0050] The nucleotide sequence of the primer of the Indel 15 marker is as follows:

[0051] InDel 15F / InDel 15R:

[0052] 5’-TCACAGCTCACAATCACAGGT-3’ / 5’-AGGGGAAGCTGGAGAAGACA-3’

[0053]The muskmelon downy mildew resistant Indel15 marker characteristic band amplified by the primers is 251bp, and its nucleotide sequence is shown in SeqID No.3;

[0054] The muskmelon downy mildew resistant Indel 15 marker characteristic band amplified by the primers is 231bp, and its nucleotide sequence is shown in SeqID No.4.

[0055] The nucleotide sequence of the Indel 20 labeled primer is as follows:

[0056] InDel 20F / InDel 20R:

[0057] 5'-TGGGGTTGCTTGTGGATAGT-3' / 5'-AGGCGAACACCTTTTGATTCA-3'

[0058] The muskmelon downy mildew resistant Indel 20 marker characte...

Embodiment 2

[0060] Example 2: A kit for screening muskmelon quality resources with resistance to muskmelon downy mildew

[0061] Further, the present invention provides a kit for screening melon quality resources with resistance to downy mildew of melon, which includes Indel-labeled primers;

[0062] The nucleotide sequence of the Indel-labeled primer is as follows:

[0063] The primer amplified and the muskmelon downy mildew resistant Indel marker characteristic band is 251bp or 231bp or 349bp or 324bp, and its nucleotide sequence is as SeqID No.3, SeqID No.4, SeqID No.7, SeqID No. 8.

[0064] The kit also includes reagents needed to perform PCR reactions and / or electrophoresis.

Embodiment 4

[0065] Embodiment four: the method for screening the melon germplasm resources with melon downy mildew resistance

[0066] Furthermore, the present invention also provides a method for screening melon germplasm resources with melon downy mildew resistance, using molecular markers InDel 15 and InDel 20 or the above-mentioned kit to carry out the following steps:

[0067] (1) Utilize the CTAB method to extract the genomic DNA of any tissue or organ of the melon sample to be tested;

[0068] (2) Using the genomic DNA of the sample to be tested obtained in step (1) as a template, using special primers for molecular markers to perform PCR amplification on different materials to obtain amplified products;

[0069] (3) carrying out polyacrylamide gel electrophoresis to the PCR amplification product of step (2) gained;

[0070] (4) Utilize silver staining method to detect the result of gel electrophoresis, observe the size of PCR amplification product;

[0071] (5) By judging the si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention specifically provides the muskmelon downy mildew-resistant InDel marker, InDel 15 and InDel 20 are adopted as molecular markers, the molecular markers and specific primers thereof obtained by the invention can be used for screening and identifying downy mildew-resistant varieties in the early stage, the identification result accuracy is high, the determination result is reliable, the breeding period can be greatly shortened, the breeding efficiency can be improved. The method has important theoretical and practical significance. And the kit for screening the muskmelon germplasm resource with muskmelon downy mildew resistance can clearly distinguish whether the muskmelon material is a downy mildew resistant variety or not by directly carrying out electrophoresis detection on a PCR amplification product, so that time and labor are saved, and the kit is not influenced by the environment.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, and specifically relates to the technical field of molecular markers for resistance to downy mildew of melon and its application. Background technique [0002] Melon (Cucumis melo L.) is an important annual horticultural economic crop of Cucurbitaceae Melon. The fruit is sweet and fragrant, and is deeply loved by consumers and sold well all over the world. Melon downy mildew is a fungal disease caused by Cuban downy mildew (Pseudoperonospora cubensis (Berk. et Curt.) Rostov.). Under high temperature and high humidity conditions, the disease spreads rapidly and seriously affects the yield and quality of melon. It is a devastating and worldwide disease. Chemical control of downy mildew not only increases planting costs and pollutes the environment, but also leads to resistance of pathogenic bacteria. Therefore, cultivating disease-resistant varieties is the fundamental way to reduce costs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张学军伊鸿平杨永李寐华凌悦铭张永兵张红杨文莉范蓉闫淼
Owner XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com