Detection model and method for rapidly detecting quality of curcuma zedoary

A detection model and technology of curcuma, which is applied in the field of rapid detection of the quality of curcuma, can solve the problems of low resolution, narrow range, spectral resolution decline, etc., and achieve rapid detection, good accuracy and robustness

Active Publication Date: 2021-06-11
GUANGDONG PHARMA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the mature desktop near-infrared spectrometer, the range of spectrum collected by the portable near-infrared spectrometer is narrower, and the number of sampling points is small, resulting in a decrease in spectral resolution. After collecting its low-resolution infrared spectr

Method used

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  • Detection model and method for rapidly detecting quality of curcuma zedoary
  • Detection model and method for rapidly detecting quality of curcuma zedoary
  • Detection model and method for rapidly detecting quality of curcuma zedoary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The prediction of embodiment 1 turmeric thrombin inhibitory rate

[0039] The method of establishing the detection model is as follows:

[0040] 1. Test the infrared spectrum curve of the curcuma sample

[0041] 1) Use a portable near-infrared spectrometer (NIR-S-R2: InnoSpectra Company, Taiwan, China) to collect spectral data of raw and vinegar zedoary samples in absorption mode at room temperature. The spectrometer weighs about 80 grams and has a spectral range of 900-1650nm. It can be connected to other devices such as smartphones or tablets via Bluetooth. The manufacturer (China, InnoSpectra Corporation, Taiwan) provides a smartphone app. China) portable near-infrared spectrometer. According to the manufacturer's instructions, the app was downloaded and installed on a smartphone running Android to collect and store spectral data in cvs format. Then, the spectrum files stored on the smartphone are uploaded to the laboratory host through wireless network. The tran...

Embodiment 2

[0062] The prediction of the clearance rate of embodiment 2 Curcuma ABTS

[0063] The method of establishing the detection model is as follows:

[0064] 1. Test the infrared spectrum curve of the curcuma sample

[0065] The method is the same as in Example 1.

[0066] 2. Test the clearance rate of ABTS of Curcuma samples

[0067] The ABTS free radical decolorization experiment belongs to the prior art, and is used to describe the free radical scavenging ability of the sample (the scavenging rate of ABTS). After ABTS (McLean) is dissolved in water to 7mmol / L, take an equal amount of ABTS solution and 140mmol / L K 2 S 2 o 8 The aqueous solution should be filtered with a 0.22um microporous membrane before mixing. The reaction solution was stored at room temperature (25±2° C.) in the dark for 12-16 hours. Since the inhibition rate should be between 20-80%, the samples with the strongest and weakest free radical scavenging ability were firstly explored through preliminary exp...

Embodiment 3

[0082] The prediction of the clearance rate of embodiment 3 curcuma DPPH

[0083] The method of establishing the detection model is as follows:

[0084] 1. Test the infrared spectrum curve of the curcuma sample

[0085] The method is the same as in Example 1.

[0086] 2. Test the scavenging rate of DPPH in the curcuma sample

[0087]The determination of the scavenging rate of DPPH belongs to the prior art. It detects the purple free radical change in the ethanol solution by spectrophotometry. When the sample presents a good free radical scavenging ability, the microplate reader can be measured by the reduction of the color The degree of reduction of the sample to free radicals, thereby obtaining the antioxidant capacity of the sample. In this study, microplates were used for one-off assays to avoid errors caused by multiple assays. The specific plan is to mix 20 μL of zedoary extract and 100 μL of 0.2mmoL / L DPPH solution into a 96-well plate, and use methanol as a control,...

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Abstract

The invention belongs to the technical field of traditional Chinese medicinal material detection, and particularly relates to a detection model and method for rapidly detecting the quality of curcuma zedoary. The detection model is established by the steps of (1) testing an infrared spectrum curve and a biological activity parameter of a curcuma zedoary sample, wherein the biological activity parameter is one of thrombin inhibition rate, ABTS clearance rate or DPPH clearance rate; (2) forming a training set and a prediction set by using the infrared spectrum curve obtained in the step (1) and the biological activity parameter; and (3) establishing a curcuma zedoary biological activity parameter detection model based on the training set and the prediction set obtained in the step (2) by using a Kennarda-Stone algorithm. The detection model obtained by the method can predict the thrombin inhibition rate, ABTS clearance rate and DPPH clearance rate of curcuma zedoary on the basis of infrared data collected by a portable near-infrared spectrometer. The detection model has the advantages of rapidness, simplicity, convenience and capability of performing field detection, and has very high application potential in traditional Chinese medicine production areas, markets, hospitals and other occasions.

Description

technical field [0001] The invention belongs to the technical field of detection of Chinese medicinal materials, and in particular relates to a detection model and a method for rapidly detecting the quality of curcuma. Background technique [0002] Curcuma has been used in Chinese medicine for thousands of years as a traditional medicine for promoting blood circulation and removing blood stasis. At present, there are decoction pieces made of raw zedoary and vinegar zedoary products processed with vinegar. The long-term clinical drug practice results show that the ability of raw zedoary to activate blood circulation and remove blood stasis is stronger than that of vinegar zedoary, but sometimes there are individual cases of vinegar zedoary with strong blood circulation. For raw curcuma, this irregular quality is due to the influence of factors such as the environment and year in the growth of the plant, and is also affected by the germplasm of the plant itself. However, the ...

Claims

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 孟江蓝振威
Owner GUANGDONG PHARMA UNIV
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