New function and use of cryptoid crab antimicrobial peptide scyreprocin

A technology of Scyreprocin and antimicrobial peptides, which can be applied in the direction of antibacterial drugs, antifungal agents, peptides, etc., can solve the problem that there is no Scyreprocin on cancer cells, and achieve good biological friendliness

Active Publication Date: 2021-06-11
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on the effect of Scyreprocin on cancer cells

Method used

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  • New function and use of cryptoid crab antimicrobial peptide scyreprocin
  • New function and use of cryptoid crab antimicrobial peptide scyreprocin
  • New function and use of cryptoid crab antimicrobial peptide scyreprocin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Determination of the concentration of antimicrobial peptide Scyreprocin inhibiting the growth of cancer cells

[0038] In this part, human cervical cancer cells (HeLa), human transitional bladder cancer cells (T24), mouse liver cells (AML12), human liver cells (L02), human non-small cell lung cancer cells (NCl-H460), human Bladder cancer cells (Du145) and human liver cancer cells (HepG2) were used as the test cells, and the activity of antimicrobial peptide Scyreprocin to inhibit cell proliferation was measured.

[0039] (a) After the cells are cultured in the corresponding cell culture medium until the cell conjugation degree is 80-90%, they are digested with EDTA-containing trypsin and resuspended with the corresponding cell culture medium, and the cell density is adjusted with the corresponding cell culture medium. Seed the cells in the culture plate and place in a cell culture incubator at 37°C in 5% CO 2 overnight culture under conditions.

[0040] (b) ...

Embodiment 2

[0043] Example 2 Detection of the apoptosis of cancer cells induced by the antimicrobial peptide Scyreprocin

[0044] In this part, human non-small cell lung cancer cells (NCl-H460), human transitional bladder cancer cells (T24), and human lung fibroblasts (HFL1) were selected as the test cells, and the antimicrobial peptide Scyreprocin induced cancer cell apoptosis. death detection.

[0045] (a) After the cells were cultured in the corresponding cell culture medium until the cell conjugation degree was 80-90%, they were digested with EDTA-containing trypsin and resuspended with the corresponding cell culture medium, and the cell density was adjusted with the corresponding cell culture medium. Seed the cells in the culture plate and place in a cell culture incubator at 37°C in 5% CO 2 overnight culture under conditions.

[0046](b) When the cells in each well grow to about 70-80% conjugation, suck off the cell culture medium, and dilute the Scyreprocin protein to 1, 5, 10, a...

Embodiment 3

[0052] Example 3 Determination of the Antimicrobial Peptide Scyreprocin Inhibiting the Growth of Cancer Cell Clones

[0053] In this part, human cervical cancer cells (HeLa), human transitional bladder cancer cells (T24), mouse liver cells (AML12), human liver cells (LO2), human non-small cell lung cancer cells (NCl-H460), human Bladder cancer cells (Du145) and human liver cancer cells (HepG2) were used as test cells to evaluate the effect of Scyreprocin on the ability of different cells to form clones.

[0054] (a) After the cells were cultured in the corresponding cell culture medium until the cell conjugation degree was 80-90%, they were digested with EDTA-containing trypsin and resuspended with the corresponding cell culture medium, and the cell density was adjusted with the corresponding cell culture medium. Inoculate 500 cells per well in the culture plate and place in a cell culture incubator at 37°C in 5% CO 2 Under the conditions, culture overnight until the cells ad...

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Abstract

New use of a cryptoid crab antimicrobial peptide Scyreprocin. The antibacterial protein is derived from a cryptoid crab, and is expressed and purified by using a genetic engineered technology. It is first disclosed that the antimicrobial peptide Scyreprocin not only has good antimicrobial activity, but also has a membrane-penetrating function, has an inhibitory and killing activity against various cancer cells, and at the same time has no cytotoxicity against human non-cancer cells. It has demonstrated that the antibacterial polypeptide has a high efficiency and specific anti-cancer activity, shows great application value, and has a good application prospect in research, development and application as an anti-tumor pharmaceutical ingredient. Provided is new use of the cryptoid crab antibacterial peptide Scyreprocin, which broadens the application range of Scyreprocin, and provides more possibilities for the application of Scyreprocin.

Description

technical field [0001] The invention relates to a new application of Scyreprocin, an antimicrobial peptide of Scylla pseudocavei. Background technique [0002] Cancer is one of the diseases with the highest morbidity and mortality in the world, and is the second leading cause of death worldwide. In addition to the recent rise of immunotherapy, effective but cheaper drug therapy is still the best choice for patients as first-line treatment. Chemotherapy remains the usual treatment modality for advanced or metastatic cancer, although localized cancers can be directly removed surgically or successfully treated with radiation therapy. Traditional chemotherapeutic drugs often lack selectivity and usually act on rapidly dividing cancer cells. They often inevitably cause damage to healthy cells and tissues, and in severe cases will lead to a series of rejection reactions in patients (such as bone marrow suppression, intestinal mucosal damage, etc.) inflammation, hair loss, etc.)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435A61K38/17A61P35/00
CPCC07K14/43509A61P35/00A61K38/00A61K38/17C07K14/435A61P31/10A61P31/04A61K38/1767
Inventor 王克坚杨盈陈芳奕陈慧芸彭会
Owner XIAMEN UNIV
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