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Process for producing alcohols with clostridium on a solid support

A solid carrier, a technology of the genus Clostridium, applied in a method of sterilization, a method of supporting/immobilizing microorganisms, immobilizing on/in an organic carrier, etc.

Pending Publication Date: 2021-06-11
INST FR DU PETROLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, such solid supports are not synthetic and pose major problems with the reproducibility of fermentation processes

Method used

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  • Process for producing alcohols with clostridium on a solid support
  • Process for producing alcohols with clostridium on a solid support
  • Process for producing alcohols with clostridium on a solid support

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] Example 1 according to the specification: Tests in batch mode with polyurethane foam

[0101] The method of batch mode was carried out experimentally: two fermentations and a control fermentation (cells in suspension) with a solid support of polyurethane foam type with different physical and structural characteristics (foam 1, foam 2). Table 1 below lists the main characteristics of the two foams:

[0102] Table 1

[0103] solid carrier foam 1 bubble 2 Aperture(mm) 0.73 1 Bulk density (g / L) 29.8 20.5

[0104] Several bioreactors were charged with 20 mL of fermentation medium which had been placed under anaerobic conditions in order to ensure the absence of oxygen. A solid volume (apparent volume) of 40% relative to the total volume of the bioreactor was introduced into each bioreactor. Initial glucose was set at 90 g / L and an inoculum rate of 10% (liquid volume) was used with cells at maximum growth rate (same inoculum size for all...

Embodiment 2

[0109] Example 2 according to the specification: Continuous testing with polyurethane foam

[0110] The method was carried out experimentally according to an embodiment of the present description, using two fermentations in continuous mode and immobilized on a solid support of polyurethane foam type (foam 1, foam 2) with different physical and structural characteristics. The main characteristics of the two foams are listed in Table 1. Filling was performed in a loose pattern with cube dimensions of 3mm x 5mm x 5mm.

[0111] The solid support was introduced into a 112 ml glass column (dimensions: inner diameter = 32 mm, H = 13.9 cm) whose volume in the column accounted for 50% of the total internal volume of the column. The column was placed in the recirculation loop of the second fermentation bottle, whose volume of fermentation medium was brought to 80 ml. The entire fermentation system was preliminarily placed under anaerobic conditions by purging with nitrogen to ensu...

Embodiment 3

[0120] Example 3 according to the specification: Continuous testing with polyurethane foam

[0121] The method was carried out experimentally according to an embodiment of the present description, using two fermentations in continuous mode and immobilized on a solid support of polyurethane foam type. Filling was performed in a loose pattern with cube dimensions of 10mm x 10mm x 7mm. The foam has a macropore size of about 1 mm.

[0122] Both fermenters were in the form of glass columns with a working volume of 250 ml. The filling conditions of the fermenters are listed in Table 4.

[0123] Table 4

[0124]

[0125] A recirculation loop is provided between the inlet and outlet of the reactor to maintain good homogenization in the fermenter. The liquid outlet is overflowing. The entire fermentation system was preliminarily placed under anaerobic conditions by purging with nitrogen to ensure the absence of oxygen. The glucose concentration in the raw material tank was...

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Abstract

The present invention relates to a process for producing alcohols, in which a sweetened fluid is introduced into a fermentation reactor (2) in order to produce a fermenting wort enriched in isopropanol, butanol, ethanol and acetone with respect to the sweetened fluid, the fermentation reactor (2) comprising a biomass produced by a strain belonging to the genus Clostridium and supported on a solid support (9) comprising a polyurethane foam. The invention also relates to a fermentation reactor (2) comprising said biomass supported on said solid support (9).

Description

technical field [0001] This specification relates to a method for the production of alcohol by fermentation of sugar liquor. Background technique [0002] To meet the challenges of the energy transition, considerable research is being conducted to develop "green" processes for obtaining chemical intermediates as alternatives to petroleum refining and / or petrochemicals. [0003] Alcohols obtained from fermentation methods, such as isopropanol and n-butanol, are the most promising alternatives to petrochemical derivatives. ABE (acetone-butanol-ethanol) fermentation by microorganisms belonging to the genus Clostridium is one of the oldest industrialized fermentations (at the beginning of the 20th century) and has been extensively studied since then. Recently, the IBE (isopropanol-butanol-ethanol) fermentation, which produces a mixture of isopropanol, butanol and ethanol and is also carried out by microorganisms belonging to the genus Clostridium, has been the subject of many s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/04C12P7/06C12P7/16C12P7/28C12N11/093C12M1/12
CPCC12M25/00C12N11/08C12P7/04C12P7/065C12P7/16C12P7/28Y02E50/10C12N11/093
Inventor H·冈萨雷斯佩纳斯M·罗帕尔斯E·托特V·库帕尔德S·梅尼尔N·洛佩斯费雷拉
Owner INST FR DU PETROLE
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