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Analysis method, adsorption prevention agent, and analysis kit

An analysis method and inhibitor technology, applied in the analysis of materials, material analysis by electromagnetic means, material separation, etc., can solve the problems of reduced precision, reduced sensitivity, and deterioration of signal-to-noise ratio

Pending Publication Date: 2021-06-15
KOBE UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are problems that these compounds do not reach the analytical column and the sensitivity decreases, or the signal-to-noise ratio (S / N ratio) deteriorates and the precision decreases

Method used

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  • Analysis method, adsorption prevention agent, and analysis kit
  • Analysis method, adsorption prevention agent, and analysis kit
  • Analysis method, adsorption prevention agent, and analysis kit

Examples

Experimental program
Comparison scheme
Effect test

no. 1 approach -

[0027] Conventionally, when a sample containing multiple compounds is subjected to LC / MS (hereinafter, "LC / MS" also includes two or more stages of mass spectrometry analysis) in the analysis of intracellular metabolites, etc., for assistance A mobile phase containing formic acid was used for ionization purposes. However, in the conventional method of using formic acid in the mobile phase, as described above, there is a problem that compounds having phosphate groups such as nucleotides are adsorbed on pipes and the like. In the analysis method of this embodiment, by performing LC / MS using a mobile phase containing oxalic acid, a compound having a phosphate group can be analyzed with good sensitivity.

[0028] (about the sample)

[0029] The sample is not particularly limited as long as it contains a compound having a phosphoric acid group. The more phosphoric acid groups this compound has, the higher its adsorption property to metals is, so the analysis method of this embodim...

Deformed example 1

[0081] The analyzer 1 of the above-described embodiment is a triple quadrupole mass spectrometer, but the configuration of the mass spectrometer 20 is not particularly limited. The mass spectrometer 20 may include a mass separation unit composed of one mass spectrometer, or may include a mass separation unit composed of two or more mass spectrometers combined differently from the above-described embodiment. For example, the analysis device 1 may include an ion trap and a time-of-flight mass separation unit.

[0082] The dissociation method is not limited to the above-mentioned CID, and can be appropriately selected according to the type of analysis device 1 such as photoinduced dissociation, electron capture dissociation, or electron transfer dissociation, and the characteristics of the molecule to be analyzed.

[0083] The present invention is not limited to the contents of the above-mentioned embodiments. Other modes conceived within the scope of the technical idea of ​​the...

Embodiment

[0085] In the following examples, the results of LC / MS / MS performed using a mobile phase containing formic acid and a mobile phase containing oxalic acid, respectively, with samples containing nucleotides of known concentration will be described.

[0086] It should be noted that the present invention is not limited to the numerical values ​​and conditions shown in the following examples.

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Abstract

This analysis method comprises performing liquid chromatography using a mobile phase containing an adsorption prevention agent for preventing adsorption of a sample containing a compound having a phosphate group on a metal, and performing mass spectrometry on the eluate from the liquid chromatography, the adsorption prevention agent containing an oxalate or a salt thereof.

Description

technical field [0001] The present invention relates to an analysis method, an adsorption inhibitor and a kit for analysis. Background technique [0002] A compound having a phosphate group plays an important role in cells and the like. For example, nucleic acid in which nucleic acid bases are linked via phosphodiester bonds of phosphate groups encodes genetic information. Adenosine triphosphate (ATP) of nucleotides participates in various reactions using the energy accumulated in the bond between phosphate groups. [0003] Analysis of compounds having a phosphate group is important for the analysis of functions of living bodies and the like, abnormalities that cause diseases, and the like. For example, by quantifying cell metabolites, information on the production and decomposition of substances in cells can be obtained. [0004] In the analysis of a sample containing a plurality of compounds such as the analysis of cell metabolites, a plurality of compounds are separate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N30/26G01N30/72G01N30/88
CPCG01N30/72G01N30/26H01J49/165H01J49/4215H01J49/005G01N30/06G01N30/7266G01N2030/027H01J49/0031H01J49/0036H01J49/0445
Inventor 藤户由佳服部考成莲沼诚久
Owner KOBE UNIV