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Bacillus subtilis strain B13 and application thereof

A technology of Bacillus subtilis and strains, which is applied to Bacillus subtilis strain B13 and its application field, can solve problems such as inability to meet market demands, and achieves the effects of meeting demands and improving fermentation yield

Pending Publication Date: 2021-06-18
INNER MONGOLIA UNIV OF TECH
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AI Technical Summary

Problems solved by technology

The efficiency of microbial production is high, and the main production strain is Bacillus. Breeding and transformation of strains have become the focus of research. In recent years, Chinese scientists have fermented riboflavin through the combination of strain selection and fermentation engineering optimization. Production has increased, but still cannot meet market demand

Method used

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Examples

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Embodiment Construction

[0015] In order to make the purpose, technical solution and advantages of the present invention clearer, the following will further describe the embodiments of the present invention.

[0016] The experimental methods in the following examples are conventional methods unless otherwise specified.

[0017] The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores.

[0018] Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0019] Embodiments of the present invention provide a Bacillus subtilis strain B13, which is obtained by the following method:

[0020] S1. Screening experimental strains: S11. Selecting the starting strain, specifically, the starting strain selected in the present invention is riboflavin-producing Bacillus subtilis (Bacillus subtilis);

[0021] S12. Take 5mL of the starting strain bacterial l...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a bacillus subtilis strain B13 and application thereof. According to the bacillus subtilis strain B13, the preservation number of the strain is CCTCC M 2020854, and the strain is preserved in the China Center for Type Culture Collection (CCTCC) on December 4, 2020. According to the bacillus subtilis strain B13 disclosed by the invention, after the strain is subjected to heavy ion mutagenesis, the shake flask fermentation yield of riboflavin is remarkably improved, and the bacillus subtilis strain B13 can be used as a strain for industrial production of enterprises and meets the requirements of the market on riboflavin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Bacillus subtilis strain B13 and an application thereof. Background technique [0002] Riboflavin, also known as vitamin B2, is a water-soluble vitamin. This yellow crystal was first isolated from whey. It was named riboflavin because of the ribitol contained in its molecular structure. Riboflavin belongs to the B vitamin family. Participate in the redox and metabolism of cells, related to the metabolism of nucleic acid, protein and fat, play an important role in the growth and development of mammals, and have a wide range of applications in the feed industry, food and pharmaceutical industries. [0003] The production methods of riboflavin are mainly divided into chemical synthesis, chemical semi-synthesis and microbial fermentation. At present, the most widely used and industrialized production is mainly microbial fermentation. Compared with other methods, it has a wi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P25/00C12R1/125
CPCC12N1/20C12P25/00
Inventor 刘占英胡建华李永丽
Owner INNER MONGOLIA UNIV OF TECH
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