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Method for identifying organic milk and conventional milk based on metabonomics

A technology of metabolomics and milk, which is applied in the direction of scientific instruments, instruments, measuring devices, etc., can solve the problems of few reports on the identification of milk, and achieve the effect of rapid identification method, simple operation method and convenient method

Active Publication Date: 2021-06-18
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology

At present, there are few reports on the identification of milk based on non-targeted metabolomics

Method used

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  • Method for identifying organic milk and conventional milk based on metabonomics
  • Method for identifying organic milk and conventional milk based on metabonomics
  • Method for identifying organic milk and conventional milk based on metabonomics

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Embodiment

[0049] equipment:

[0050] Chromatography: sciex eXionlc TM system

[0051] Mass spectrometry: sciex triple TOF TM 6600 system

[0052] Columns: Agilent Eclipse Plus C18 (30 × 150, 1.8um)

[0053] Low temperature high speed centrifuge (EppendROF)

[0054] Vortex oscillator

[0055] Ultra pure water instrument (Milli-Q)

[0056] Reagent consumables:

[0057] Acetonitrile (mass spectrometry, Merck-Millipore)

[0058] Formic acid (chromatography pure, dikma)

[0059] Ammonium acetate (chromatography, SIMCA)

[0060] Material: Buy a commercially available organic certified Hutten cattle organic milk and conventional Nurtin milk powder manufactured by conventional production lines.

[0061]1. Sample pretreatment:

[0062] Accurately weigh 0.50 g of sample in 10 ml of centrifuge tube, add 1.5 ml of deionized water, reverse mix; at 4 ° C 5000 rpm 25min, transfer 1 ml of the middle liquid in another clean centrifuge tube; add 4 ml of acetonitrile, vortex shock 12min Centrifuge at 4 ° C 90...

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Abstract

The invention provides a method for identifying organic milk and conventional milk based on metabonomics, and relates to the technical field of milk identification and detection. The method comprises the following steps: (1) carrying out sample pretreatment on organic milk and conventional milk under the same condition; (2) carrying out data acquisition on the pretreated sample through high performance liquid chromatography-tandem high-resolution mass spectrometry to obtain a metabolite detection multimodal map; (3) performing peak extraction and correction on the chromatographic peak to obtain the relative peak area and retention time of all metabolites; (4) carrying out principal component analysis and orthogonal partial least square analysis, screening differential markers, and obtaining an identification model to distinguish organic milk and conventional milk; and (5) comparing the difference marker with an online database for identification. The method disclosed by the invention can be used for rapidly and comprehensively detecting and identifying the milk products, the detection result is accurate, and the operation method is simple.

Description

Technical field [0001] The present invention relates to the field of milk identification and testing, in particular, involving a method of identifying organic milk and conventional milk based on metabolic. Background technique [0002] Milk is a daily health food with a high nutritional value, containing a variety of organic components. Milk contains a variety of vitamins, amino acids, and phospholipids, is an ideal drink. [0003] Organic milk is absolutely prohibited from using pesticides, fertilizers, hormones, growth regulators, and feed additives in production and processing, emphasizing "complete natural and non-pollution". Organic milk produced by organic cows can maximize the natural nutritional ingredients of milk, and the extent of organic milk will greatly improve. [0004] The production process of organic milk is much more rare than green food, and it is necessary to produce and process according to the production system of organic agriculture. Conventional milk is a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8634
Inventor 陈爱亮张娟李会赵燕徐贞贞陈刚杨曙明
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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