A method for measuring the ability of tannin extract to complex protein by spectrophotometry
A spectrophotometry and extraction technology, which is applied in the field of chemical analysis and achieves the effects of low equipment requirements, good stability and simple technical operation.
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Embodiment 1
[0020] Example 1: Determination of the protein complexing ability of gallnut tannin extract
[0021] The gallnut tannin extract was prepared with methanol solution into tannin solutions with mass percentage concentrations of 0.2, 0.4, 0.8, 1.2, and 1.6 mg / mL, respectively; the mass percentage concentration was 1 mg prepared with pH4.9 phosphate buffer. / mL of bovine serum albumin (BSA) solution; add 900 μL of BSA solution and 100 μL of tannin solution of a series of concentrations to a 1 mL cuvette, mix thoroughly, and stand at room temperature for 1 min, then measure the concentration of the reaction solution at a wavelength of 510 nm. Absorbance value, take the reaction buffer as blank; take the tannin concentration (mg / mL) in the reaction solution as the abscissa, and the absorbance value as the ordinate to obtain a linear simulation equation (see figure 1 ), the ability of tannins to complex proteins is judged by the slope of the linear equation (see Table 1). The larger t...
Embodiment 2
[0022] Example 2: Determination of the ability of sorghum tannin extract to complex protein
[0023] The sorghum tannin extract was prepared into a series of tannin solutions with mass percentage concentrations of 0.2, 0.4, 0.8, 1.2, and 1.6 mg / mL with methanol solution; the mass percentage concentration was 1 mg prepared with pH4.9 phosphate buffer. / mL of bovine serum albumin (BSA) solution; add 900 μL of BSA solution and 100 μL of tannin solution of a series of concentrations to a 1 mL cuvette, mix thoroughly, and stand at room temperature for 1 min, then measure the concentration of the reaction solution at a wavelength of 510 nm. Absorbance value, take the reaction buffer as blank; take the tannin concentration (mg / mL) in the reaction solution as the abscissa, and the absorbance value as the ordinate to obtain a linear simulation equation (see figure 2 ), the ability of tannins to complex proteins was judged by the slope of the linear equation (see Table 1).
Embodiment 3
[0024] Example 3: Determination of the ability of plum tannin extract to complex protein
[0025] The plum tannin extract was prepared with methanol solution into a series of tannin solutions with mass percentage concentrations of 0.2, 0.4, 0.8, 1.2, and 1.6 mg / mL; the mass percentage concentration was 1 mg prepared with pH4.9 phosphate buffer. / mL of bovine serum albumin (BSA) solution; add 900 μL of BSA solution and 100 μL of tannin solution of a series of concentrations to a 1 mL cuvette, mix thoroughly, and stand at room temperature for 1 min, then measure the concentration of the reaction solution at a wavelength of 510 nm. Absorbance value, take the reaction buffer as blank; take the tannin concentration (mg / mL) in the reaction solution as the abscissa, and the absorbance value as the ordinate to obtain a linear simulation equation (see image 3 ), the ability of tannins to complex proteins was judged by the slope of the linear equation (see Table 1).
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