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Preparation method of colloidal gold immunochromatography test strip

An immunochromatographic test strip, colloidal gold technology, applied in analytical materials, biological tests, material testing products, etc., can solve the problems of incomplete release, small amount of gold-labeled binding pads, and great influence of the matrix, reducing physical and chemical Influence of properties, improvement of physical and chemical properties, effect of stability improvement

Pending Publication Date: 2021-06-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the labeling methods of gold label pads are to spray the marked concentrated gold particles on the glass cellulose membrane. During use, it is found that the gold label binding pad prepared by this method has a small amount of labeling, and the release is not complete during the detection process. , affecting the accuracy and sensitivity of the test results
The sample pad also needs to be pretreated before use. The matrix in the sample pad has a great influence. When it is directly used for detection without treatment, there are major problems, such as: false positive and false negative reactions, low negative-positive contrast, It cannot effectively promote the release of gold particles in the gold-labeled binding pad, etc.

Method used

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  • Preparation method of colloidal gold immunochromatography test strip

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Embodiment 1

[0028] A preparation method for a colloidal gold immunochromatographic test strip, comprising the following steps:

[0029] 1) Material selection: choose glass cellulose film and polyester film, and store in a cool place away from light.

[0030] 2) Soaking: Add the pretreatment soaking solution into the container, and immerse the glass cellulose membrane and the polyester membrane for 30s respectively. The pretreatment soaking solution includes the following components in mass percentage: disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose : 1%, Tween-20: 0.2%, dissolved in ultrapure water.

[0031] 3) Drying: move the infiltrated glass cellulose membrane and polyester membrane to 37°C for drying respectively.

[0032] 4) Preparation of gold-labeled binding pad: label the corresponding monoclonal antibody with the prepared colloidal gold solution, block the labeled monoclonal antibod...

Embodiment 2

[0036] A preparation method for a colloidal gold immunochromatographic test strip, comprising the following steps:

[0037] 1) Material selection: choose glass cellulose film and polyester film, and store in a cool place away from light.

[0038] 2) Soaking: Add the pretreatment soaking solution into the container, and immerse the glass cellulose membrane and the polyester membrane for 30s respectively. The pretreatment soaking solution includes the following components in mass percentage: disodium hydrogen phosphate: 0.142%, potassium dihydrogen phosphate: 0.027%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose : 1%, Tween-20: 0.2%, dissolved in ultrapure water.

[0039] 3) Drying: move the infiltrated glass cellulose membrane and polyester membrane to 37°C for drying respectively.

[0040] 4) Preparation of gold-labeled binding pad: label the corresponding monoclonal antibody with the prepared colloidal gold solution, block the labeled monoclonal antibod...

Embodiment 3

[0043] A preparation method for a colloidal gold immunochromatographic test strip, comprising the following steps:

[0044] 1) Material selection: choose glass cellulose film and polyester film, and store in a cool place away from light.

[0045] 2) Soaking: Add the pretreatment soaking solution into the container, and immerse the glass cellulose membrane and the polyester membrane for 30s respectively. The pretreatment soaking solution includes the following components in mass percentage: disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose : 1%, Tween-20: 0.2%, dissolved in ultrapure water.

[0046] 3) Drying: move the infiltrated glass cellulose membrane and polyester membrane to 37°C for drying respectively.

[0047] 4) Preparation of gold-labeled binding pad: label the corresponding monoclonal antibody with the prepared colloidal gold solution, block the labeled monoclonal antibod...

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Abstract

The invention relates to the technical field of immunochromatography detection, and discloses a preparation method of a colloidal gold immunochromatography test strip, which comprises the following steps: respectively soaking a glass cellulose membrane and a polyester membrane in a pretreatment soaking solution, and drying; sealing, centrifuging and concentrating the labeled monoclonal antibody, spraying the concentrated colloidal gold solution onto a polyester film, and drying to prepare a gold-labeled conjugate pad; and assembling the colloidal gold conjugate pad and the glass cellulose membrane into the colloidal gold immunochromatography test strip. The pretreatment soak solution can effectively remove matrixes in a commodity gold mark pad and a sample pad, and improve the physicochemical properties of the gold mark pad and the sample pad; the test strip treated by the pretreatment soak solution disclosed by the invention is marked by colloidal gold particles and is uniformly released, and the stability of a gold-labeled conjugate is greatly improved.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to a method for preparing a colloidal gold immunochromatography test strip. Background technique [0002] Colloidal gold immunochromatography technology is a technology that combines antigen-antibody immune reaction with colloidal gold labeling and tracing technology for qualitative and quantitative detection of antigen and antibody content. The colloidal gold labeling of antibodies is mainly firmly combined through the electrostatic force between colloidal gold and proteins. Due to its advantages of fast, simple, low cost, and good stability, it has opened a new door for on-site rapid detection. the gate. [0003] Gold label pads and sample pads are important components of colloidal gold immunochromatography test paper, and the pretreatment of gold label pads and sample pads has varying degrees of influence on subsequent detection. The main function of the ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/543G01N33/58
CPCG01N33/558G01N33/543G01N33/587
Inventor 王秉闫琳马维维胡智文万军民
Owner ZHEJIANG SCI-TECH UNIV
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