Double-layer micro-fluidic chip for antibody detection and preparation method of double-layer micro-fluidic chip

Abstract

The invention discloses a double-layer micro-fluidic chip for antibody detection and a preparation method thereof. The chip sequentially comprises a reaction layer substrate, a micro-cavity sample adding layer and a sealing cover layer from bottom to top, wherein the reaction layer substrate comprises a glass slide substrate and a glutaraldehyde substrate assembled on the glass slide substrate, a strip-shaped detection band and a quality control band are printed on the glutaraldehyde substrate; a plurality of through holes which are through up and down are formed in the microcavity sample adding layer; the plurality of through holes are in one-to-one correspondence with the plurality of strip-shaped detection bands and the plurality of quality control bands to form a plurality of reaction tanks; a plurality of micro-channel grooves are formed in the bottom of the sealing cover layer, the plurality of micro-channel grooves are in one-to-one correspondence with the plurality of reaction tanks, and an inflow hole and an outflow hole which vertically penetrate through the sealing cover layer are respectively formed in two ends of each micro-channel groove. The chip disclosed by the invention can realize rapid, sensitive and accurate high-throughput detection of the platelet antibody.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a double-layer microfluidic chip for antibody detection and a preparation method thereof. Background technique [0002] With the significant increase of patients with tumors and blood system diseases and the rapid development of clinical transfusion medicine, the amount of blood transfusion is increasing year by year. Blood transfusion is an effective measure to increase platelet count, prevent bleeding, and protect the life safety of patients. Its safe, scientific and effective transfusion has become an inevitable requirement for the development of clinical blood transfusion technology. However, at present, most blood transfusions still use random infusion. Improper blood is quickly destroyed and cleared by the body's immune system after infusion into the patient's body, resulting in frequent occurrence of platelet transfusion ineffectiveness, which not only wastes pr...

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Problems solved by technology

[0004] When the above method is used for qualitative detection of antibodies, its sensitivity and specificity are only 30%-40%. There are human errors in the interpretation of the results, and it takes about several hours to complete the detection, which cannot meet the rapid needs of clinicians. At the same time, this technology has no corresponding In order to overcome the practical problems of other clinical antibody detection methods such as low sensitivity, poor specificity, complicated clinical operation, long detection time, difficult result interpretation, and lack of corresponding reagent quality control; the existing microcolumn gel method Antibody detection indicates that red blood cells are usually treated with chemical reagents (such as glutaraldehyde) to formaldehyde, and then coated with anti-human IgG to indicate the reaction results. Aldehydated erythrocytes destroy the antigen structure, which is easy to cause missed detection, so the use is limited, and it is difficult to meet the clinical requirements for rapid and safe blood matching

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