Unlock instant, AI-driven research and patent intelligence for your innovation.

Rapid propagation method for tissue culture high-quality seedlings of Musa coccinea

A technology of tissue culture and safflower banana, applied in the field of plant tissue culture, can solve the problems of high pollution rate, no safflower banana, slow reproduction speed, etc., and achieve the effect of high application value, simple and affordable technology

Active Publication Date: 2021-06-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the natural state, Acacia safflower is mainly propagated by ramets, the propagation speed is slow, and it is difficult to meet the needs of the market. The use of plant tissue culture technology can effectively solve this problem, but when the underground rhizomes of Acacia safflower are used as explants , high pollution rate
[0003] At present, there is no report on the propagation of safflower seedlings by tissue culture at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 20 seconds, then disinfect with 0.1% mercury chloride solution for 8 minutes, rinse with sterile water for 4 times, then disinfect with 0.1% mercury chloride solution for 4 minutes, rinse with sterile water for 5 times, and insert the callus In the tissue induction medium, the culture temperature is 24° C., the light intensity is 1500 lx, and the light is 16 hours / day. After 40 days of culture, there are calluses and somatic embryos at the base of the explants, and the sterilization success rate is 85%. Each liter of callus induction medium contains: 6-benzylpurine (6-BA) 1.0 mg, thidiazuron (TDZ) 0.5 mg, kinetin (KT) 1.0 mg, coconut water 100 ml, sucrose 20 g, agar 6 g, the bala...

Embodiment 2

[0026] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 30 seconds, then disinfect with 0.1% mercury solution for 9 minutes, rinse with sterile water for 5 times, then disinfect with 0.1% mercury solution for 3 minutes, rinse with sterile water for 4 times, insert the callus In the tissue induction medium, the culture temperature is 28°C, the illuminance is 2000lx, and the light is 14 hours / day. After 35 days of culture, there are calluses and somatic embryos at the base of the explants, and the success rate of disinfection is 90%. Each liter of callus induction medium contains: 1.5 mg 6-benzylpurine (6-BA), 0.3 mg thidiazuron (TDZ), 1.5 mg kinetin (KT), 75 ml coconut water, 25 g sucrose, agar 6.5 grams, the balance is MS medium, pH 5...

Embodiment 3

[0032] 1. The acquisition and disinfection method of explants: select the unopened young flower buds of the excellent line of Musa coccinea Andr. which grow vigorously and have no damage by diseases and insect pests in the growing season, and first soak them in 75% alcohol by volume fraction. Soak for 40 seconds, then disinfect with 0.1% mercury liter solution for 10 minutes, rinse with sterile water for 5 times, then disinfect with 0.1% mercury solution for 2 minutes, rinse with sterile water for 5 times, insert the callus In the tissue induction medium, the culture temperature is 30°C, the illuminance is 2500 lx, and the light is 12 hours / day. After 30 days of culture, there are callus and somatic embryos at the base of the explants, and the sterilization success rate is 95%. Each liter of callus induction medium contains: 6-benzylpurine (6-BA) 2.0 mg, thidiazuron (TDZ) 0.2 mg, kinetin (KT) 2.0 mg, coconut water 50 ml, sucrose 30 g, agar 7 g, the balance being MS medium, pH ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rapid propagation method for tissue culture high-quality seedlings of Musa coccinea. The rapid propagation method for the tissue culture high-quality seedlings of the Musa coccinea is characterized in that unbloomed tender flower buds of excellent strains of the Musa coccinea are used as explants, and culture medium formulas and culture conditions suitable for all culture stages are selected through the stages of obtaining and disinfecting the explants, proliferating and differentiating calluses, differentiating somatic embryos to form plants, carrying rooting and strong seedling culture on adventitious buds, transplanting test-tube seedlings and the like; the large-scale propagation of the seedlings of the Musa coccinea is successfully carried out, and the propagated high-quality seedlings of the Musa coccinea meet the requirements of the market. According to the rapid propagation method for the tissue culture high-quality seedlings of the Musa coccinea, the technology is simple, affordable, feasible and high in application value; the method can be implemented only by simple plant tissue culture equipment.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation of high-quality seedlings of safflower tissue culture. Background technique: [0002] Safflower banana (Musa coccinea Andr.), also known as red banana, zhitian banana, etc., is a perennial evergreen herbaceous plant in the Musa family, native to eastern Yunnan, Guangdong, Guangxi and other places in China. Banana safflower has a chic plant shape, large inflorescences, bright red bracts, and long-lasting flowering. It is an ornamental flower with high ornamental value. In warm regions such as South China, it can be planted in the corner of the courtyard, in front of the window, on a rockery, at the entrance of a pavilion or by a pool And other places, very full of southern characteristics, can also be potted for viewing; its bright and straight red erect inflorescences are also excellent flower arrangement materials, and can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君李琳吴坤林房林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI