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Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme

An endogenous, tobacco technology, applied in the application, tobacco, treatment of tobacco and other directions, can solve problems such as unreported, achieve the effects of reasonable process, promotion of tobacco leaf alcoholization, good economic and social benefits

Pending Publication Date: 2021-06-25
YUNNAN REASCEND TOBACCO TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the use of waste fresh tobacco as raw materials, the integration of low-temperature extraction and separation processes, the development of tobacco endogenous total enzymes, and their application in tobacco leaf quality improvement and alcoholization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for preparing endogenous total enzymes from fresh tobacco comprises the following steps:

[0026] (1) Raw material treatment: Wash the discarded fresh tobacco raw materials, squeeze out the juice with a press, quickly filter with gauze to remove suspended plant particles, and then use a centrifuge at 3000r / min for 60min to remove impurities, and collect the supernatant for later use ;

[0027] (2) Concentration by ultrafiltration: Concentrate the supernatant in ultrafiltration equipment, the molecular weight cut-off (MWCO) of the ultrafiltration membrane is 10kD, the temperature is 4°C, and the pressure is 0.10MPa, so that the liquid volume is concentrated to about 20% of the original volume;

[0028] (3) Precipitation and refinement: cool the concentrated solution to 0-2°C, add edible ethanol with a mass concentration of 95% while stirring until the mass concentration of ethanol in the mixed solution is 10%, leave it to stand to precipitate the enzyme, remov...

Embodiment 2

[0033] The method of using fresh tobacco to prepare endogenous total enzymes is to wash the discarded fresh tobacco raw materials, squeeze out the juice with a press, quickly filter out suspended plant particles with gauze, and then use a centrifuge at 15000r / min for 10min to remove impurities and collect Serum for later use. Concentrate the supernatant in an ultrafiltration device with an ultrafiltration membrane molecular weight cut-off (MWCO) of 50kD, a temperature of 35°C, and a pressure of 0.40MPa to concentrate the liquid volume to 19% of the original volume. Cool the concentrated solution to 2-4°C, add edible ethanol while stirring until the mass concentration of ethanol in the mixed solution is 90%, let it stand to allow the enzyme to precipitate, remove the supernatant, collect the precipitate to obtain the wet enzyme. The wet enzyme is frozen at a low temperature below 0°C for 24 hours to be dried, and then pulverized to obtain the tobacco endogenous total enzyme pro...

Embodiment 3

[0037] The method of using fresh tobacco to prepare endogenous total enzymes is to wash the discarded fresh tobacco raw materials, squeeze out the juice with a press, quickly filter out suspended plant particles with gauze, and then use a centrifuge at 4000r / min for 30min to remove impurities and collect them. Serum for later use. Concentrate the supernatant in an ultrafiltration device with an ultrafiltration membrane molecular weight cut-off (MWCO) of 20kD, a temperature of 25°C, and a pressure of 0.20MPa to concentrate the liquid volume to 21% of the original volume. Cool the concentrated solution to 3°C, add ethanol while stirring until the mass concentration of ethanol in the mixed solution is 20%, let it stand still to allow the enzyme to precipitate, remove the supernatant, collect the precipitate to obtain the wet enzyme. The wet enzyme is frozen at a low temperature below 0°C for 4 hours, dried, and then pulverized to obtain the tobacco endogenous total enzyme product...

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PUM

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Abstract

The invention discloses a method for preparing endogenous total enzyme by using fresh tobacco and an application of the endogenous total enzyme. The method comprises the following steps: cleaning waste fresh tobacco raw materials, squeezing to obtain juice, filtering to remove suspended plant particles, centrifuging to remove impurities, collecting a supernate, carrying out ultrafiltration concentration on the supernate, cooling a concentrated solution to 0-4 DEG C, adding ethanol while stirring till that the mass concentration of the ethanol in the mixed solution is 10-90%, standing, removing the supernatant, and collecting a precipitate to obtain wet enzyme; and freezing the wet enzyme at a low temperature of below 0 DEG C, drying, and crushing to obtain a tobacco endogenous total enzyme product. The endogenous total enzyme is used as a tobacco leaf quality-improving agent to be applied to a feeding process of a cigarette cut tobacco production line, or is used as a tobacco leaf mellowing agent to be applied to a secondary moistening link of a threshing and redrying line or an export feeding process. A tobacco endogenous bio-enzyme prepared by the method is good in product quality, and the preparation method is simple. The endogenous total enzyme is applied to quality improvement and alcoholization of tobacco leaves, the smoke quality of tobacco products can be improved, and the alcoholization time of the tobacco leaves can be shortened.

Description

technical field [0001] The invention relates to the technical field of preparation of tobacco additives, in particular to a method for preparing endogenous total enzymes by using waste fresh tobacco raw materials, and the application of endogenous total enzymes in improving the quality and aging of tobacco leaves. Background technique [0002] my country is the largest tobacco producing and consuming country in the world, among which the output of tobacco leaves in Yunnan Province accounts for about 40% of the country's total. Due to the high standard of flue-cured tobacco leaves, about 1 million tons of discarded fresh tobacco raw materials will be produced in the tobacco field every year after the flue-cured tobacco leaves are harvested. Waste fresh tobacco raw materials mainly refer to top leaves, bottom leaves, fireworks, tobacco stems, tobacco stems produced during the planting process of flue-cured tobacco, and fresh tobacco leaves with stunted structure, rigid structu...

Claims

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Application Information

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IPC IPC(8): A24B15/26A24B15/30
CPCA24B15/26A24B15/307
Inventor 刘煜宇刘丽芬包秀萍李艳李红霞包崇彦罗建飞陈俊宇李国盛徐兰兰念小魁刘维涓
Owner YUNNAN REASCEND TOBACCO TECH GRP