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Method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells by sodium new houttuyfonate

A technology of NCI-H1299 and new houttuyniacin sodium, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, anhydride/acid/halide active ingredients, etc., can solve problems such as defects in theory and application, and achieve The effect of obvious clinical application value

Inactive Publication Date: 2021-06-25
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are still flaws in the theory and application of new houttuyfonate sodium in the mechanism of lung adenocarcinoma NCI-H1299 cells

Method used

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  • Method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells by sodium new houttuyfonate
  • Method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells by sodium new houttuyfonate
  • Method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells by sodium new houttuyfonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Annexin V / PI flow cytometry cell death detection after Houttuydin Sodium acted on NCI-H1299 cells.

[0025] Experimental method: Normal cultured NCI-H1299 cells were mixed with 5×10 5 pcs / ml density, 2ml / well seeded into 6-well plate. After 24 H adherent culture, the culture medium was replaced with monoculture with neohouttuydin sodium concentration of 0, 0.1, 0.2 and 0.4 mM. After 24 H intervention, the cells were collected, and the cell death was detected by using the FITC-Annexin V flow cytometry kit.

[0026] Experimental results: if Figure 1a ~ Figure 1e As shown, the death of lung adenocarcinoma NCI-H1299 cells increases with the dose of neohouttuydin sodium, and the concentration dependence increases, indicating that neohouttuyfonate sodium has a significant inhibitory effect on lung adenocarcinoma NCI-H1299 cells.

Embodiment 2

[0028] The inflammatory factors IL-1β and IL-18 were detected in NCI-H1299 cells treated with Houttuyfonate Sodium.

[0029] Experimental method: Normal cultured NCI-H1299 cells were mixed with 5×10 5 pcs / ml density, 2ml / well seeded into 6-well plate. After 24 H adherent culture, the culture medium was replaced with monoculture with neohouttuydin sodium concentration of 0, 0.1, 0.2 and 0.4 mM. After 24 H intervention, the cells were collected, and the release of inflammatory factors IL-1β and IL-18 was detected by using ELISA detection kits for IL-1β and IL-18.

[0030] Experimental results: if Figure 2a ~ Figure 2b As shown, the release of inflammatory factors IL-1β and IL-18 in lung adenocarcinoma NCI-H1299 cells increased in a concentration-dependent manner with the dose of neohouttuyfonate sodium, indicating that neohouttuyfonate sodium had an effect on lung adenocarcinoma NCI- H1299 cells have a significant release of pro-inflammatory factors, and the release of IL-1...

Embodiment 3

[0032] Immunofluorescence detection of ASC protein in NCI-H1299 cells treated with Houttuyfonate Sodium.

[0033] Experimental method: Normal cultured NCI-H1299 cells were mixed with 5×10 5 pcs / ml density, 2ml / well seeded into the laser confocal dish. After 24 H adherent culture, the culture medium was replaced with monoculture with neohouttuydin sodium concentration of 0, 0.1, 0.2 and 0.4 mM. After 24H intervention, fix and incubate the ASC protein primary antibody overnight on a horizontal shaker. Incubate the fluorescent secondary antibody, observe and take pictures under the laser confocal microscope.

[0034] Experimental results: if image 3 As shown, neohouttuyfonate sodium acts on lung adenocarcinoma NCI-H1299 cells to form ASC scars, indicating that neohouttuyfonate sodium can promote the oligomerization of ASC protein in lung adenocarcinoma NCI-H1299 cells, and contribute to cell pyroptosis. typical characteristics.

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Abstract

The invention relates to a method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells through a traditional Chinese medicine monomer sodium new houttuyfonate, and belongs to the field of traditional Chinese medicine monomer application, sodium new houttuyfonate promotes the lung adenocarcinoma NCI-H1299 cells to release inflammatory factors IL-1beta and IL-18, inflammasome NLRP3 is used as a mediation, and through ASC protein oligomerization, caspase1, IL-1beta and GSDMD protein shearing, the classical path of pyroptosis is activated, thereby conducing pyroptosis of NCI-H1299 cells. The sodium new houttuyfonate can be used for inhibiting tumor growth of a lung adenocarcinoma model mouse caused by NCI-H1299 cells, so that the sodium new houttuyfonate is expected to be used as an active component for preparing the lung adenocarcinoma resisting medicine, and has obvious clinical application value.

Description

technical field [0001] The invention relates to a method for inducing pyroptosis of lung adenocarcinoma NCI-H1299 cells by new houttuyfonate sodium, which belongs to the application field of traditional Chinese medicine monomers. Background technique [0002] Lung cancer is one of the most aggressive types of cancer globally with a very high mortality rate. The latest statistical results show that although the number of deaths from lung cancer has declined rapidly in the past 26 years, the death rate of lung cancer cases currently exceeds the sum of the death rates of breast cancer, prostate cancer and colorectal cancer that follow closely behind. Thus, lung cancer remains the deadliest cancer subtype. [0003] Traditional Chinese medicine monomers are a treasure trove of natural screening anti-tumor compounds. Houttuynia cordata belongs to the lung meridian, which is a commonly used drug for treating lung diseases in ancient China, and it is also a commonly used drug for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/185A61P35/00A61K36/78
CPCA61K31/185A61P35/00A61K36/78
Inventor 蒋日磊许家佗刘平
Owner SHANGHAI UNIV OF T C M
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