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Chimeric recombinant PRRSV DIVA vaccine strain cDY56 for porcine reproductive and respiratory syndrome virus

A technology for respiratory disorders and vaccine strains, applied in recombinant DNA technology, viruses, viral peptides, etc., and can solve problems such as poor results

Pending Publication Date: 2021-06-29
SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes a new type of porcine reproductive and respiratory syndrome chimeric recombination for the problem that existing attenuated vaccines, inactivated vaccines and various subunit vaccines have been proven to be ineffective. Construction method and application of PRRSV DIVA vaccine strain cDY56

Method used

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  • Chimeric recombinant PRRSV DIVA vaccine strain cDY56 for porcine reproductive and respiratory syndrome virus
  • Chimeric recombinant PRRSV DIVA vaccine strain cDY56 for porcine reproductive and respiratory syndrome virus
  • Chimeric recombinant PRRSV DIVA vaccine strain cDY56 for porcine reproductive and respiratory syndrome virus

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1, such as Figure 1-14 shown.

[0048] 1.1 Main experimental materials

[0049] Marc-145 cells, BHK cells, competent cells DH5α, PRRSV DY strain, PRRSV XZ strain, vector pBluescript II SK(+),  pcDNA3.1(+), pCI-neo, and the 5' end sequence of PRRSV DY strain genome ( 1bp-5388bp) recombinant plasmid pSK-1 and 3' end sequence (12201bp-15442bp) recombinant plasmid pSK-4 were prepared and preserved by the author's laboratory.

[0050] 1.2 Main reagents Plasmid extraction kits, gel recovery kits, RNA extraction kits, and large plasmid extraction kits were purchased from China Omega Bio-Tek Inc. Company; T4 DNA ligase, reverse transcription kit, PCR polymerase, restriction endonuclease, NEBuilder HiFi DNA Assembly Master Mix / NEBuilder HiFi DNA Assembly CloningKit were purchased from the United States New England Biolabs company; X-treme GENE HP DNA Transfection Reagent was purchased from Switzerland Roche Company; peroxidase-labeled goat anti-pig IgG, FITC-labe...

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Abstract

The invention belongs to the technical field of veterinary biological products, and relates to a construction method and application of a chimeric recombinant PRRSV DIVA vaccine strain cDY56 for a porcine reproductive and respiratory syndrome virus. A genotype-II classical strain DY is used as a parent strain, and ORF5 and ORF6 genes of a highly pathogenic PRRSV XZ strain are used for replacing corresponding genes of the parent strain to construct a chimeric recombinant PRRSV vaccine strain cDY56. Compared with current commercialized vaccines, the chimeric recombinant PRRSV strain cDY56 provided by the invention has better effects in the aspects of inducing humoral immunity and cellular immunity, reducing immunosuppression and the like, thereby having a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and relates to a construction method and application of PRRSV DIVA vaccine strain cDY56 chimeric and recombined with porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) was first discovered in the United States in 1987. At present, the disease has become prevalent all over the world, causing significant economic losses to the pig industry. Worldwide, vaccines are widely used to protect or reduce the damage caused by PRRSV. However, both attenuated and inactivated vaccines, as well as various subunit vaccines, have been shown to be ineffective and provide only partial protection. In recent years, people have tried various adjuvants in an attempt to improve the poor immune protection effect of PRRSV vaccines. There are many reasons for the poor immune protection effect of PRRSV vaccines, amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/40C12N15/63C12N15/64
CPCC12N7/00C07K14/005C12N2770/10021C12N2770/10022C12N2770/10034
Inventor 张松林刘鸿艳李峰刘磊马永彪公鑫婧沈志强
Owner SHANDONG BINZHOU ANIMAL SCI & VETERINARY MEDICINE ACADEMY
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