Micro-droplet fluorescence signal detection device using mixed exciting light as light source

Abstract

The invention provides a micro-droplet fluorescence signal detection device. The micro-droplet fluorescence signal detection device comprises a light source synthesis module for synthesizing a plurality of lasers with different wavelengths into mixed exciting light; an objective lens which is located on a light conduction path of the mixed exciting light and can confocal the mixed exciting light on the micro-droplet to be detected so as to excite and generate a plurality of different fluorescence signals corresponding to different lasers, and collimate the plurality of different fluorescence signals into parallel light; an optical processing module which comprises a convex lens and an optical fiber, and the first end of the optical fiber is located on the focus of the side, away from the objective lens, of the convex lens so as to receive and transmit a plurality of different fluorescence signals focused on the convex lens; and a signal receiving module used for processing a plurality of different fluorescence signals received and transmitted by the optical fiber. The optical fiber is adopted as a rear-end fluorescence collection and transmission component, the loss of fluorescence signals is effectively reduced, the optical processing module and the signal receiving module are independently connected through the optical fiber, the signal receiving module can be randomly arranged in the space, and the arrangement flexibility of the system is improved.

Description

technical field [0001] The invention belongs to the technical field of microfluidic detection chips, and in particular relates to a micro-droplet fluorescence signal detection device using mixed excitation light as a light source. Background technique [0002] Biochips are widely used in new drug development, disease diagnosis and gene expression analysis. Microfluidic detection chip technology is becoming more and more mature, and has gradually become a hot spot of people's attention. There are various biological and chemical processes in the microfluidic detection chip, and the process is usually completed in the micron-scale flow channel space, which requires some devices that can detect the reaction process. [0003] In terms of detection methods, it can be divided into two types: CCD time-sharing photography and laser single-point frequency division detection. Compared with laser single-point scanning systems, CCD photography has a simpler structure, shorter developmen...

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Problems solved by technology

[0003] In terms of detection methods, it can be divided into two types: CCD time-sharing photography and laser single-point frequency division detection. Compared with laser single-point scanning systems, CCD photography has a simpler structure, shorter development cycle, and lower technical difficulty. Disadvantages such as slow detection speed, high cost, low sensitivity, and large space occupation. In future applications, it will be limited by the diversity of biological samples and the performance of the instrument itself, making it difficult to apply in large-scale batches.

In the traditional laser confocal detection device, the detection optical path is to eliminate the interference of stray light outside the focal plane and improve the detection sensitivity. A pinhole is set at the receiving end, and its spatial structure is single and fixed. Whether it is single laser, double laser or multi-laser Excitation signal detection devices all have disadvantages such as a fixed increase in the number of pinholes, an increase in the number of lasers used for excitation light, an increase in the optical path, and a high requirement for the integrity of the detection device from the laser to the signal collection end. Because the increase in the number of pinholes makes debugging more difficult Large, which virtually increases the signal receiving error of the device. When performing multi-channel signal detection, part of the signal is attenuated and lost due to the increase of the spatial optical path, and the corresponding detection signal is lost. The high integrity requirements are not convenient for product assembly. and post-maintenance

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