High-stability inactivated virus preserving fluid and preparation method thereof

Abstract

The invention provides a high-stability inactivated virus preserving fluid and a preparation method thereof. The preserving fluid is prepared from split salt, an Rnase enzyme inhibitor, trometamol, ethylenediamine tetraacetic acid, glycerin, nucleic acid protection peptide and water, the Rnase enzyme inhibitor is prepared from guanidine isothiocyanate, phenol, sodium citrate, dithiothreitol and sodium lauroyl sarcosinate, the Rnase enzyme inhibitor comprises the following components in parts by weight: 16 parts of guanidine isothiocyanate, 2 parts of phenol, 10 parts of sodium citrate, 1 part of dithiothreitol and 1 part of sodium lauroyl sarcosinate, and the protection liquid is prepared from the following components in parts by weight: 9 parts of split salt, 20 parts of the Rnase enzyme inhibitor, 14 parts of trometamol, 3 parts of ethylenediamine tetraacetic acid, 10 parts of glycerol, 6 parts of nucleic acid protection peptide and 100 parts of water. The virus protein is cracked by the cracking salt to inactivate the virus protein, so that the safety in the transportation process is improved, meanwhile, the Rnase enzyme inhibitor is used for protecting the virus nucleic acid from being degraded, and the extraction efficiency of the preserved virus nucleic acid is improved.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a highly stable inactivated virus preservation solution and a preparation method thereof. Background technique [0002] The virus preservation solution is used for the collection, preservation and transportation of common virus samples such as new coronavirus, influenza virus, hand-foot-mouth virus, etc. When the sampling tube is submerged in the sample swab virus sample, it is a liquid that protects the virus to be tested. It can collect throat swabs, Nasal swabs or tissue samples from specific parts, stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification. [0003] Many inactivated virus preservation solutions have been developed now. After a large number of searches and references, we found that the existing preservation solutions are as disclosed by the publication numbers KR1020100015613A, KR1020170095270A and KR1020100...

AI Technical Summary

Problems solved by technology

However, the preservation solution will still destroy part of the nucleic acid, and the integrity of the viral nucleic acid still needs to be improved

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