Electrochemical luminescence aptamer sensor for specific detection of chloramphenicol as well as preparation method and application of electrochemical luminescence aptamer sensor
A technology of aptamer sensor and chloramphenicol, applied in the direction of material electrochemical variables, etc., can solve the problem of narrow detection range
Active Publication Date: 2021-07-06
CHANGZHOU UNIV
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Problems solved by technology
At present, in the prior art, there are some related electrochemiluminescence aptasensors, among which CN201911323033.9 is an electrochemiluminescence aptasensor and a method for detecting chloramphenicol, and Ti3 is prepared Electrochemiluminescence aptasensor modified by C2-ZnO nanocomposite material is used for the detection of chloramphenicol, the detection range is 0.1ng / mL~100ng / mL, and the minimum detection limit is 0.019ng / mL, narrow detection range
Method used
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Embodiment 1
[0035] (1) BP / PTC-NH 2 Preparation of composite materials:
[0036] Disperse 5 mg of black phosphorus lumpy solid into 1 mL of NMP, grind for 20 min, and disperse the mixture in a small glass bottle containing 4 mL of NMP. After being carefully sealed, the vial was sonicated at 100 W in an ice bath for 8 h, and the resulting dispersion was centrifuged at 7000 rpm for 20 min to obtain the supernatant, and finally centrifuged at 12000 rpm for 20 min to obtain a BPQDs suspension.
[0037] Dissolve 0.2g of PTCDA in 10mL of acetone, add 2mL of ethylenediamine and 6mL of black phosphorus quantum dot suspension, stir for 2h under ice-water bath, centrifuge, wash and dry to obtain a reddish-brown powder, that is, BP / PTC- NH 2 , and disperse 5 mg of composite material in 10 mL of water for use.
[0038] (2) Preparation of Co-Ni / MOF composites:
[0039] 0.262g Co(NO 3 ) 2 ·6H 2 O and 0.291g Ni(NO 3 ) 2 ·6H 2 O was dissolved in 30mL of methanol to obtain solution A, and 0.164g ...
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Abstract
The invention provides an electrochemical luminescence aptamer sensor for specific detection of chloramphenicol as well as a preparation method and application of the electrochemical luminescence aptamer sensor, which particularly belongs to the field of electrochemical luminescence detection. The method comprises the following steps of (1) preparing a black phosphorus doped PTC-NH2 composite material (BP / PTC-NH2) and Co-Ni / MOF, (2) preparing an electrochemical luminescence aptamer sensor, and (3)jointly modifying BP / PTC-NH2 and Co-Ni / MOF to the surface of a glassy carbon electrode to improve the sensitivity and stability of electrochemical luminescence, then loading an aptamer to obtain the electrochemical luminescence aptamer sensor. The sensor can specifically recognize chloramphenicol, the detection range is 1.0*10<-13> mol / L to 1.0*10<-6> mol / L, and the lowest detection limit is 2.9*10<-14> mol / L. The method for detecting chloramphenicol has the advantages of high sensitivity, strong specificity and simple operation.
Description
technical field [0001] The invention belongs to the field of electrochemiluminescence detection, and relates to an electrochemiluminescence aptamer sensor for specific detection of chloramphenicol and a detection method thereof. Especially related to the loading of aptamer molecules on Co-Ni / MOF / BP / PTC-NH 2 Modified glassy carbon electrode surface, namely apt / Co-Ni / MOF / BP / PTC-NH 2 The / GCE electrode is a sensing element, and it is an electrochemiluminescence analysis method for quantitative detection of chloramphenicol in river water. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP) is the first broad-spectrum antibiotic that can be produced in large quantities through chemical synthesis. It has the advantages of stable drug properties and low price, and has a good effect on infectious diseases caused by Gram-negative bacteria. treatment effect. Based on this, chloramphenicol is widely used in the treatment of human and animal bacterial diseases, as we...
Claims
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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 陈智栋温静单学凌蒋鼎王文昌
Owner CHANGZHOU UNIV