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Electrochemiluminescence aptasensor for detecting kanamycin and preparation method thereof

An aptamer sensor, kanamycin technology, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, scientific instruments, etc., can solve the problems of difficult on-site detection, complicated experimental operation, large error of results, etc., and achieve detection Low cost, good selectivity and high sensitivity

Active Publication Date: 2021-11-02
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the commonly used methods for detecting kanamycin mainly include high performance liquid chromatography, enzyme-linked immunosorbent assay, liquid chromatography, gas chromatography, electrochemical method, etc., but these methods generally have complicated experimental operations. And time-consuming and other shortcomings, it is difficult to promote to the market for on-site testing
[0004] Existing aptamer sensors have high detection sensitivity, but the detection cost is high, the measurement time is long, and the result error is large

Method used

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  • Electrochemiluminescence aptasensor for detecting kanamycin and preparation method thereof
  • Electrochemiluminescence aptasensor for detecting kanamycin and preparation method thereof
  • Electrochemiluminescence aptasensor for detecting kanamycin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Assembly of an electrochemiluminescence aptasensor for the detection of kanamycin

[0054] (1) Preparation of PTC-Cys and Au@HKUST-1 materials:

[0055] S1. Preparation of precursor PTCA, dissolve 0.1g PTCDA in 20mL aqueous solution containing 0.04g NaOH, then slowly add 1M HCl solution to the above solution until a bright red precipitate is formed, wash the precipitate with deionized water Several times, until the pH value of the suspension is about 7, dry it for later use. Accurately weigh 5 mg of the above-prepared PTCA, disperse it in 10 mL of deionized water, and ultrasonically disperse evenly, stir in an ice-water bath for 30 min, add 40 mg of EDC and 10 mg of NHS (m / m=4:1), and continue stirring for 2 h to Activate the carboxyl group, then add 100 mg of cysteine, stir overnight, and finally, collect the product by centrifugation as PTC-Cys; disperse PTC-Cys in DMF to make it evenly dispersed, and obtain PTC-Cys with a concentration of 1 mg / mL DMF dispersion...

Embodiment 2

[0071] (1) Assembling the electrochemiluminescence aptasensor for detecting kanamycin in Example 1

[0072] Step (1) During the preparation of the PTC-Cys material, in the amidation reaction of S1, the ratio of EDC to NHS was changed to 1:1 (m / m), and the others were the same as in Example 1.

Embodiment 3

[0074] In Example 1 (1) assembling an electrochemiluminescent aptasensor for detecting kanamycin, in step (2), the concentration of the aptamer in the aptamer solution is 2-10 μM.

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Abstract

The invention belongs to the field of electrochemiluminescence detection, and in particular relates to an electrochemiluminescence aptamer sensor for detecting kanamycin and a preparation method thereof. Load the aptamer on the surface of the composite material Au@HKUST‑1 / PTC‑Cys modified glassy carbon electrode, and use the Au‑S bond between Au@HKUST‑1 and PTC‑Cys to jointly modify the surface of the glassy carbon electrode, The sensitivity and stability of the electrochemiluminescence are significantly improved, and then the aptamer is loaded to obtain an electrochemiluminescence aptasensor, which can specifically recognize the target molecule kanamycin, and the selectivity of kanamycin detection is improved. The detection range of the electrochemiluminescent aptasensor of the present invention is 1.0×10 ‑13 ~1.0×10 ‑8 M, the lowest detection limit is 4.2×10 ‑14 M. The preparation method of the modified electrode is simple, the detection sensitivity to kanamycin is high, the selectivity is good, and the linear range is wide.

Description

technical field [0001] The invention belongs to the field of electrochemiluminescence detection, and in particular relates to an electrochemiluminescence aptamer sensor for detecting kanamycin and a preparation method thereof. Background technique [0002] Food safety issues have seriously affected people's lives. It is very important to monitor food safety. Kanamycin (KAN), an aminoglycoside antibiotic, is potentially toxic, and excessive use of KAN can cause serious side effects in humans and animals, including ototoxicity, nephrotoxicity, and anaphylactic shock, affecting consumers health. In order to improve people's awareness of the hazards of antibiotic residues and effectively protect human health, the European Union has clearly stipulated that the maximum residue limit (MRL) of kanamycin in milk is 150 μg kg -1 , due to the unreasonable use of kanamycin will lead to residues in meat, dairy products and other animal-derived foods, these residues will enter the huma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N21/76
CPCG01N27/3278G01N27/308G01N21/76
Inventor 陈智栋周利君单学凌蒋鼎王文昌
Owner CHANGZHOU UNIV