Tungsten sulfide-black titanium dioxide heterojunction-based method for detecting 5fC via photoelectrochemical analysis

A technology of black titanium dioxide and photoelectrochemistry, which is applied in the direction of electrochemical variables of materials, material analysis by electromagnetic means, and analysis of materials, can solve the problems of complex operation, expensive instruments, and high cost, and achieve simple detection methods, low cost, and improved The effect of detection sensitivity

Active Publication Date: 2019-08-27
SHANDONG AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

However, these methods generally have the disadvantages of expensive instruments, complicated operatio

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  • Tungsten sulfide-black titanium dioxide heterojunction-based method for detecting 5fC via photoelectrochemical analysis
  • Tungsten sulfide-black titanium dioxide heterojunction-based method for detecting 5fC via photoelectrochemical analysis
  • Tungsten sulfide-black titanium dioxide heterojunction-based method for detecting 5fC via photoelectrochemical analysis

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preparation example Construction

[0054] (2) Preparation of thin-layer tungsten sulfide nanosheets: Take 50-300mg bulk tungsten sulfide and 20-100mg polyacrylic acid and add them to 50-100mL water, ultrasonically oscillate for 2-10h, and centrifuge the dark green dispersion at 3000-5000rpm for 10 -20 minutes, collect the supernatant, continue to centrifuge the supernatant at 9000-12000rpm for 10-40 minutes, collect the solid, wash several times with deionized water, and vacuum freeze-dry.

[0055] (3) Preparation of AuNPs: Put 50-100 ml of double-distilled water into a double-necked flask, add 50-100 mM HAuCl 4 Solution 2-10mL, when the solution starts to reflux, remove the stopper. Quickly add 2-10 mL of 35-40 mM sodium citrate and replace the stopper, allowing the system to reflux for an additional 20-40 minutes. The heat was turned off and the system was allowed to cool to room temperature (20-25° C.) with stirring. Store the prepared gold nanoparticles in a refrigerator at 4°C for future use.

[0056] (...

Embodiment 1

[0074] Example 1: Thin layer WS 2 Preparation of nanosheets

[0075] Add 100mg block tungsten sulfide and 50mg polyacrylic acid into 80mL water, ultrasonically shake for 4 hours, centrifuge the dark green dispersion at 4000rpm for 20 minutes, collect the supernatant, continue to centrifuge the supernatant at 9000rpm, collect the solid, and use deionized Washed several times with water and freeze-dried in vacuum.

Embodiment 2

[0076] Example 2: Preparation of AuNPs

[0077] Put 80 ml of double-distilled water into a double-necked flask, add 60 mM HAuCl 4Solution 5mL, when the solution began to reflux, remove the stopper. Quickly add 5 mL of 38.8 mM sodium citrate, replace the stopper, and allow the system to reflux for an additional 20 minutes. The heat was turned off and the system was allowed to cool to room temperature (20-25° C.) with stirring. Store the prepared gold nanoparticles in a refrigerator at 4°C for future use.

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Abstract

The invention discloses a tungsten sulfide-black titanium dioxide heterojunction-based method for detecting 5fC via photoelectrochemical analysis. According to the method, a photoelectrochemical biosensor for detecting the 5fC is firstly constructed; and the photoelectrochemical biosensor comprises an electrode, and a thin-layer tungsten sulfide nanosheet, AuNPs, 4-amino-3-hydrazino-5-sulfydryl-1,2,4-triazole, 5fC and black titanium dioxide, which are modified on the surface of the electrode in sequence. According to the method, the good photoelectric activity of WS2, the effect of the AuNPs on accelerating the electron transfer, and the specific covalent reaction of a diamine group on 4-amino-3-hydrazino-5-sulfydryl-1,2,4-triazole and an aldehyde group on the 5fC are utilized, and the black titanium dioxide is used as a signal amplification molecule, so that the sensitive detection for 5-formylcytosine is realized. The detection method provided by the invention is high in operation stability, strong in sensitivity, low in detection limit and convenient to realize miniaturization, and is capable of realizing the rapid and sensitive detection for the 5fC through only modifying the surface of the ITO electrode.

Description

technical field [0001] The invention relates to the technical field of photoelectrochemical analysis, in particular to a method for detecting 5fC by photoelectrochemical analysis based on tungsten sulfide-black titanium dioxide heterojunction. Background technique [0002] In epigenetics, DNA methylation, in which cytosine is converted to 5-methylcytosine (5mC), acts as a special epigenetic modification in the regulation of gene expression, histone modification, chromosomal recombination, development It plays a key regulatory role in a series of life activities including regulation and disease pathogenesis. Maintaining the dynamic balance of DNA methylation in the process of DNA methylation and demethylation is crucial to the growth and development of mammals. In 2009, researchers reported that oxidases of the TET (ten-eleven-translocation, 10-11 translocation) family can catalyze 5-methylcytosine to form 5-hydroxymethylcytosine. Hydroxymethylcytosine has attracted more an...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 周云雷李菲陈燕殷焕顺艾仕云
Owner SHANDONG AGRICULTURAL UNIVERSITY
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