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Kit for evaluating allergy degree of individual

A kit and individual technology, applied in the field of kits for evaluating the degree of individual allergy, can solve the problem of lack of efficient and unified standards for the diagnosis of allergies, and achieve the effect of improving efficiency and accuracy and wide application.

Active Publication Date: 2021-07-13
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there is no efficient and unified standard for the diagnosis of allergies

Method used

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  • Kit for evaluating allergy degree of individual
  • Kit for evaluating allergy degree of individual

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Aspartic acid (ASP) is used to assess human seafood allergy in the following steps:

[0024] (1) Acquisition of samples:

[0025] Three volunteers with a history of mollusc seafood allergy and 11 healthy volunteers were recruited. The healthy group and the allergic group were matched in gender and age and were comparable (P>0.05).

[0026] (2) Pretreatment of samples:

[0027] Dry the feces sample in an oven at 60°C and crush it. Accurately weigh 50 mg of the dried solid sample and put it into a hydrolysis tube. Try to avoid the sample powder hanging on the wall. Slowly add 4ml of hydrochloric acid with a concentration of 6mol / L and blow it with nitrogen. Seal the hydrolysis tube immediately after blowing nitrogen for 15 minutes; place the sealed sample in an oven at 110°C for 22-24 hours and take it out to cool; open the hydrolysis tube and dilute the sample to 50ml with pure water; 1ml of the solution sample was deacidified on a nitrogen blower at 60°C until dry; a...

Embodiment 2

[0036] The detection steps of aspartic acid (ASP) for evaluating the sensitization of mouse TM (shrimp tropomyosin) are as follows:

[0037] (1) Acquisition of samples:

[0038] Nine female Balb / c mice aged 6-8 weeks were fed, and three of them were sensitized with TM. The mice were stimulated to defecate after stress, and the feces of the two groups of mice were collected.

[0039] (2) Pretreatment of samples:

[0040]Dry the feces sample in an oven at 60°C and crush it. Accurately weigh 50 mg of the dried solid sample and put it into a hydrolysis tube. Try to avoid the sample powder hanging on the wall. Slowly add 4ml of 6mol / L hydrochloric acid and hydrolyze with a nitrogen blower. Seal the tube immediately after blowing the tube with nitrogen for 15 minutes; place the sealed sample in an oven at 110°C for 22-24 hours and take it out to cool; open the hydrolysis tube and dilute the sample to 50ml with pure water; accurately absorb the solution after the volume 1ml of the ...

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Abstract

The invention discloses a kit for evaluating the allergy degree of an individual. The allergy degree is evaluated by detecting the aspartic acid content in excrement of the individual, and the kit comprises a reagent for detecting the aspartic acid content in the excrement. According to the invention, aspartic acid (ASP) is used as a biomarker for evaluating the degree of allergy. The amino acid biomarker can be used for screening allergy individuals, can be applied to preparation of allergy individual screening products and the like, and provides a direction for development of allergy detection reagents and kits. The amino acid biomarker can be used as a target spot for screening and diagnosing the allergic reaction occurrence risk, optimizes the traditional detection and evaluation method, improves the allergy screening and diagnosis efficiency and accuracy, and can also be used as a target spot for monitoring the allergy development process of an allergy patient; the abnormal change of the amino acid in the allergic split excrement provides a new direction for researching an allergic pathogenic mechanism in the future, and the application is wide.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for evaluating the degree of individual allergy. Background technique [0002] In recent years, allergy has seriously affected the normal life of 5% of adults and 8% of infants in the world, and its prevalence has gradually increased. Common food groups such as peanuts, tree nuts, fish, shellfish, eggs, milk, wheat and beans contribute to many of the more serious disease burdens. However, there is currently no effective way to prevent and treat allergies. Studies have shown that there are many factors that can affect the incidence of allergies, such as allergens, geographical environment, dietary structure, ethnic differences, age and gender, etc. Therefore, there are many different epidemiological data of allergies in the same region or different regions, the same population or different populations, which increases the difficulty of allergy research and prevention. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6812G01N2800/24
Inventor 傅玲琳王彦波王翀马瑾雅陈剑
Owner ZHEJIANG GONGSHANG UNIVERSITY
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