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Method for synchronously identifying floating algae and benthic algae in aquatic ecosystem

An ecosystem and benthic algae technology, applied in the field of molecular biology, can solve problems such as specific amplification and identification of prokaryotic algae, influence composition and ecological function exploration and evaluation, etc., to improve species annotation effect, repeatable high sex effect

Active Publication Date: 2021-07-23
PEKING UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although this method can identify eukaryotic algae in environmental samples, it cannot specifically amplify and identify prokaryotic algae, which affects the exploration and evaluation of the species composition and ecological functions of all algae in the environmental system

Method used

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  • Method for synchronously identifying floating algae and benthic algae in aquatic ecosystem
  • Method for synchronously identifying floating algae and benthic algae in aquatic ecosystem
  • Method for synchronously identifying floating algae and benthic algae in aquatic ecosystem

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Embodiment

[0031] In this embodiment, the water body of a drinking water source is taken as an example, and the planktonic algae and benthic algae in the water body are monitored and identified by using the method of the present invention.

[0032] 1. Sample collection and pretreatment

[0033] Four monitoring stations were selected for sampling, and the water samples collected from the four stations were marked as W1-W4, and the corresponding sediment samples were marked as S1-S4, respectively, and the species of algal communities in each sample were monitored.

[0034] Surface water was collected from each monitoring site separately, mixed to obtain 5L surface water samples, refrigerated and transported back to the laboratory at 4°C, and all collected water samples were covered with 0.22 μm polycarbonate membranes (Millipore, USA) within 24 hours. ), and store the filter membrane carrying the filtrate in a -80°C refrigerator.

[0035] At each monitoring station, 20 mL of surface sedim...

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Abstract

The invention provides a method for synchronously identifying floating algae and benthic algae in an aquatic ecosystem. The method comprises the following steps: S1, collecting a water sample and a sediment sample from the aquatic ecosystem; S2, respectively extracting and purifying DNA from the water sample and the sediment sample; S3, carrying out PCR (Polymerase Chain Reaction) amplification by taking the purified DNA as a template; S4, performing high-throughput sequencing on a PCR amplification product to obtain an algae ASV representative sequence; S5, establishing an algae species annotation database; and S6, comparing the algae ASV representative sequence with the sequence data of the algae species annotation database, and carrying out species annotation to obtain algae species composition information. According to the method, the species composition of all eukaryotic and prokaryotic algae in a water body and sediments in the ecological system is efficiently, quickly and synchronously identified through a high-throughput sequencing technology on the basis of the difference of genetic materials of the algae.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a method for synchronously identifying planktonic algae and benthic algae in aquatic ecosystems based on high-throughput sequencing technology. Background technique [0002] Algae (Algae) refers to a group of thallus plants capable of photosynthesis, self-supporting life, no vascular bundles, and no real differentiation of roots, stems and leaves. Algae are the most important primary producers in aquatic ecosystems such as oceans, rivers, lakes, and reservoirs, and play an important role in the biogeochemical cycles of elements such as carbon, nitrogen, phosphorus, sulfur, and silicon. Algae are the basis of the ecosystem food chain and provide a basic guarantee for the species diversity in the ecosystem. Algae have a wide distribution range, short growth cycle, and are very sensitive to environmental changes such as nutrients, light, temperature, and distur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00Y02A40/80
Inventor 王佳文陈倩倪晋仁刘宇心刘清香
Owner PEKING UNIV
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