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Tissue culture bud proliferation and rooting synchronous culture method for Alpinia zerumbet cv. Variegata

A technology of synchronous cultivation and galangal group, applied in the direction of culture medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of unseen bud proliferation and rooting synchronization, etc., and achieves shortened cultivation period, strong operability, and high growth rate. robust effect

Inactive Publication Date: 2021-07-23
广西壮族自治区南宁良凤江国家森林公园
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above reports are two-step culture of subculture and rooting, and there is no research report on the synchronization of bud proliferation and rooting

Method used

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  • Tissue culture bud proliferation and rooting synchronous culture method for Alpinia zerumbet cv. Variegata
  • Tissue culture bud proliferation and rooting synchronous culture method for Alpinia zerumbet cv. Variegata
  • Tissue culture bud proliferation and rooting synchronous culture method for Alpinia zerumbet cv. Variegata

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Embodiment 1

[0031] A method for synchronous culture of tissue culture bud proliferation and rooting of Alpinia officinalis Alpinia officinalis, comprising the following steps:

[0032] (1) Pretreatment: When the weather is clear for 3 consecutive days, pick up robust Alpinia officinalis plants, wash the root soil, and culture the whole plant in tap water for 3 days, change the water once a day, and then cut off all the leaves and roots , cutting the rhizomes with axillary buds to obtain axillary bud rhizomes, each section of axillary bud rhizomes is 4 cm long, then soaked in 5‰ washing powder solution, and scrubbed with a soft brush for 5 minutes, and then washed 3 times with pure water;

[0033] (2) Disinfection and sterilization on the surface of explants: put the axillary bud rhizomes obtained in step (1) on the ultra-clean workbench, disinfect with 75% alcohol solution for 30 seconds, then wash with sterile water for 3 times, and then use a mass fraction of 1.25‰ of HgCl 2 The soluti...

Embodiment 2

[0037] A method for synchronous culture of tissue culture bud proliferation and rooting of Alpinia officinalis Alpinia officinalis, comprising the following steps:

[0038] (1) Pretreatment: When the weather is clear for 3 consecutive days, pick up robust Alpinia officinalis plants, wash the root soil, and culture the whole plant in tap water for 3 days, change the water once a day, and then cut off all the leaves and roots , cutting the rhizomes with axillary buds to obtain axillary bud rhizomes, each section of axillary bud rhizomes is 3 cm long, then soaked in 5‰ washing powder solution, and scrubbed with a soft brush for 5 minutes, and then washed 3 times with pure water;

[0039] (2) Disinfection and sterilization on the surface of explants: put the axillary bud rhizome obtained in step (1) on the ultra-clean workbench, disinfect with 75% alcohol solution for 30 s, then wash with sterile water for 4 times, and then use the mass fraction 1.25‰ of HgCl 2 The solution was s...

Embodiment 3

[0043] A method for synchronous culture of tissue culture bud proliferation and rooting of Alpinia officinalis Alpinia officinalis, comprising the following steps:

[0044] (1) Pretreatment: When the weather is clear for 3 consecutive days, pick up robust Alpinia officinalis plants, wash the root soil, and culture the whole plant in tap water for 3 days, change the water once a day, and then cut off all the leaves and roots , cutting the rhizomes with axillary buds to obtain axillary bud rhizomes, each section of axillary bud rhizomes is 5 cm long, then soaked in 5‰ washing powder solution, and scrubbed with a soft brush for 5 minutes, and then washed 3 times with pure water;

[0045] (2) Disinfection and sterilization of the explant surface: put the axillary bud rhizome obtained in step (1) on the ultra-clean workbench, sterilize it with 75% alcohol solution for 30 s, then wash it with sterile water for 4 times, and then use the mass fraction 1.25‰ of HgCl 2 Soak in the solu...

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Abstract

The invention discloses a tissue culture bud proliferation and rooting synchronous culture method for Alpinia zerumbet cv. Variegata. The method comprises four steps of pretreatment, explant surface disinfection and sterilization and primary culture of buds, bud proliferation and rooting synchronous culture and nursery stock transplantation, and is characterized in that subculture buds are cut into cluster buds, every 3-5 buds form a cluster, the cluster buds are inoculated into a bud proliferation and rooting culture medium, and the culture is performed for 35-40 days, and the proliferation coefficient is 5-12; and clumpy buds with multiple roots and strong buds are transplanted, and the clumpy buds with multiple small buds and multiple short and few roots are continuously cultured in a bud proliferation and rooting synchronous culture medium. According to the bud proliferation and rooting synchronous culture method, the growth stages of proliferation, seedling strengthening, rooting culture and the like are synchronously completed by using one culture medium, the steps and cost of rooting culture are saved, the seedling culture period is shortened, and the method is a high-efficiency and low-cost production technology for the tissue culture seedlings of Alpinia zerumbet cv. Variegata and is worthy of application and popularization in production.

Description

technical field [0001] The invention belongs to the technical field of asexual rapid propagation of plants, and in particular relates to a method for synchronous cultivation of tissue-cultured bud proliferation and rooting of Alpinia officinalis. Background technique [0002] Galanga mosaic ( Alpinia zerumbet cv. Variegata, also known as variegated Alpinia officinalis, is a variant of Alpinia officinalis in the family Zingiberaceae. Alpinia mosaic is a perennial herb with leaves up to 1m long and dark green with golden spots. It is a commonly used foliage and flower viewing plant for indoor potted plants and landscaping. Because of its bright leaf color, beautiful flower posture, pure flower fragrance and high ornamental value, it is deeply favored by operators. [0003] The propagation of Algalpina mosaic seedlings mainly adopts the tillering and branching method at present. This propagation method is limited by climatic factors and grows slowly, which is difficult to mee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/25A01G24/15
CPCA01H4/001A01H4/008A01G24/15A01G24/25
Inventor 刘芳余注光田湘俞建妹廖克波陈碧珍赵春梅刘玉军沈遐梁小春廖敏彤
Owner 广西壮族自治区南宁良凤江国家森林公园
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