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Tissue stem cell separation and function evaluation system

A technology for evaluating systems and stem cells, which is applied in the field of stem cell separation, and can solve problems such as the separation liquid is not pure enough, error-prone, and the operating environment is uncontrolled

Pending Publication Date: 2021-07-23
杭州憶盛医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the existing defects and provide a tissue stem cell separation and functional evaluation system to solve the above-mentioned background technology. In the prior art, cell stem cells are mostly separated by manual operation, which is difficult to accurately Control the amount of normal saline and stem cell stock solution. Manual operation causes the operating environment to be generally uncontrolled, which will affect the separation liquid that is not pure enough. Using manual operation will result in low separation efficiency and error-prone problems.

Method used

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  • Tissue stem cell separation and function evaluation system

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Effect test

Embodiment 1

[0042] A tissue stem cell isolation and functional evaluation system, comprising the following steps:

[0043] Step 1: Put male mice and female mice together to mate, rest the fertilized female mice for a few days, obtain oocytes from the fertilized mice, reprogram, activate and culture the obtained oocytes in vitro Blastocyst formation;

[0044] Step 2: activating the oocytes by using a calcium ionophore at a concentration of 5 μM-15 μM, and then treating the oocytes with 6-dimethylaminopurine at a concentration of 1 μM-5 μM;

[0045] Step 3: Carry out in vitro culture by using two different mediums of G1.2 medium and SNUnt-2. After the culture is completed, remove the zona pellucida or part thereof from the blastocyst, and then remove the trophoblast from the obtained blastocyst to separate the inner cell mass;

[0046] Step 4: Put the obtained blastocysts into the centrifugal device for centrifugation, and put the stem cell separation fluids obtained by centrifugation at di...

Embodiment 3

[0058] A tissue stem cell isolation and functional evaluation system, comprising the following steps:

[0059] Step 1: Put male mice and female mice together to mate, rest the fertilized female mice for a few days, obtain oocytes from the fertilized mice, reprogram, activate and culture the obtained oocytes in vitro Blastocyst formation;

[0060] Step 2: activating the oocytes by using a calcium ionophore at a concentration of 5 μM-15 μM, and then treating the oocytes with 6-dimethylaminopurine at a concentration of 1 μM-5 μM;

[0061] Step 3: Carry out in vitro culture by using two different mediums of G1.2 medium and SNUnt-2. After the culture is completed, remove the zona pellucida or part thereof from the blastocyst, and then remove the trophoblast from the obtained blastocyst to separate the inner cell mass;

[0062] Step 4: Put the obtained blastocysts into the centrifugal device for centrifugation, and put the stem cell separation fluids obtained by centrifugation at ...

Embodiment 4

[0074] A tissue stem cell isolation and functional evaluation system, comprising the following steps:

[0075] Step 1: Put male mice and female mice together to mate, rest the fertilized female mice for a few days, obtain oocytes from the fertilized mice, reprogram, activate and culture the obtained oocytes in vitro Blastocyst formation;

[0076] Step 2: activating the oocytes by using a calcium ionophore at a concentration of 5 μM-15 μM, and then treating the oocytes with 6-dimethylaminopurine at a concentration of 1 μM-5 μM;

[0077] Step 3: Carry out in vitro culture by using two different mediums of G1.2 medium and SNUnt-2. After the culture is completed, remove the zona pellucida or part thereof from the blastocyst, and then remove the trophoblast from the obtained blastocyst to separate the inner cell mass;

[0078] Step 4: Put the obtained blastocysts into the centrifugal device for centrifugation, and put the stem cell separation fluids obtained by centrifugation at ...

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Abstract

The invention discloses a tissue stem cell separation and function evaluation system. The tissue stem cell separation and function evaluation system comprises the following steps: placing a male mouse and a female mouse together for mating, resting the fertilized female mouse for several days to obtain oocytes in the fertilized mouse body, and reprogramming, activating and culturing the obtained oocytes in vitro to form embryonic vesicles; after embryonic vesicles are cultured, removing zona pellucida or parts of the zona pellucida from the embryonic vesicles, then removing trophoblasts from the obtained embryonic vesicles, and separating inner cell clusters. The gene sequence of the mouse is similar to that of human beings, the similarity between the whole genome of the mouse and the whole genome of the human beings is extremely high, similar characters of many diseases which are difficult to cure by the human beings can be found on the mouse body, disease treatment genes are found through experiments, and the function of each component after tissue stem cell separation is easier to obtain.

Description

technical field [0001] The invention belongs to the technical field of stem cell separation, and in particular relates to a tissue stem cell separation and function evaluation system. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. According to the developmental potential of stem cells, they can be divided into three types: Classes: totipotent stem cells, pluripotent stem cells and unipotent stem cells (multipotent stem cells). Stem cells are not fully differentiated and immature cells that have the potential to regenerate various organs and the human body. They are called "universal stem cells" in the medical field. cell". [0003] In the existing technology, manual operation is often used for cell stem cell separati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12Q1/02A01K67/027
CPCC12N5/0606G01N33/5044A01K67/0271C12N2500/14C12N2500/40C12N2503/02G01N2500/10A01K2207/12A01K2267/03
Inventor 彭伟群曹小伍曹得明李禅华国明
Owner 杭州憶盛医疗科技有限公司
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