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Primer for anti-poaching identification of antelope and application of primer

A takin, fluorescent quantitative technology, applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc., can solve the time-consuming and labor-intensive identification of takin anti-poaching, and achieve improved identification speed, high reliability, The effect of improving sensitivity

Active Publication Date: 2021-07-23
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a primer for takin anti-poaching identification and its application to solve the time-consuming and laborious technical problem of takin anti-poaching identification in the prior art

Method used

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  • Primer for anti-poaching identification of antelope and application of primer
  • Primer for anti-poaching identification of antelope and application of primer
  • Primer for anti-poaching identification of antelope and application of primer

Examples

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Embodiment 1

[0051] This embodiment provides a primer for takin anti-poaching identification, including Takin-F and Takin-R, the specific sequence is:

[0052] Takin-F: 5'-TTCTGCGATTCTGCTTCGTG-3';

[0053] Takin-R: 5'-CCAGACCCGATGAACACCAG-3'.

[0054] In this embodiment, the specific method for obtaining Takin-F and Takin-R includes the following steps:

[0055] Step 1: extract total RNA from takin muscle tissue, send the extracted total RNA to Lianchuan Biotechnology Co., Ltd. for sequencing, and sort out multiple non-conserved cDNA sequences according to the sequencing results provided by the sequencing company;

[0056] Step 2, carry out sequence alignment on the NCBI website with the non-conserved cDNA sequence sequence described in step 1, sequence alignment uses NCBI website (URL is http s: / / www.ncbi.nlm.nih.gov / ) from With the bla stn online tool and the Nucleotide collection (nr / nt) database, after the sequence comparison is completed, find the non-conserved cDNA sequence with th...

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Abstract

The invention provides a primer for anti-poaching identification of antelope and an application of the primer. The primer comprises Takin-F and Takin-R, and the specific sequence of the primer is Takin-F: 5'-TTCTGCGATTCTGCTTCGTG-3'; and Takin-R: 5'-CCAGACCCGATGAACACCAG-3'. A method comprises the following steps: carrying out transcriptome sequencing on total RNA of antelope muscle tissues, finding out an unconservative cDNA sequence according to a transcriptome sequencing result, determining a target cDNA sequence by utilizing a related biological analysis tool, designing specific primers Takin-F and Takin-R aiming at the target cDNA sequence, and identifying antelope muscle tissue by using the specific primers Takin-F and Takin-R, so that the identification speed and the reliability of the identification result are improved. When the primers Takin-F and Takin-R are used for identifying the antelope muscle tissue specificity, a fluorescent quantitative PCR method is adopted, the identification sensitivity, specificity and accuracy are improved, besides, the identification speed is increased, and the identification result is good in observation repeatability, good in intuition and high in reliability.

Description

technical field [0001] The invention belongs to the technical field of animal molecular biology, relates to takin anti-poaching identification, in particular to a primer for takin anti-poaching identification and an application thereof. Background technique [0002] Takin (Budorcas taxicolor) is a first-class protected animal in my country. It inhabits high-altitude and high-cold cliff areas. It is large in size and often becomes the target of illegal poaching. Its distinction from cattle, sheep, etc., has great limitations. It is not only time-consuming and labor-intensive to identify takins by relying on traditional classification and morphological characteristics, but also often interfered by subjective factors, which is very easy to confuse and make mistakes. In addition, traditional identification methods are difficult to carry out targeted Identification of handicapped individuals. Contents of the invention [0003] Aiming at the deficiencies in the prior art, the pur...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6851C12Q2600/166C12Q2521/107C12Q2531/113C12Q2545/101
Inventor 李安宁郭睿李一丹昝林森马征
Owner NORTHWEST A & F UNIV