A method for inducing garlic polyploidy or mutant

Abstract

The invention discloses a method for inducing garlic polyploidy or mutant, comprising the following steps: first, cutting the garlic bulb along one side of the abdomen of the garlic bulb to 3-8 mm above the root, and then removing the scale buds on the inner side of the bulb, Until the growth point at the bottom of the root base is exposed; then the garlic cloves are induced with a mutagen; then the induced garlic cloves are directly planted in the field to obtain garlic polyploids or mutants; or the induced garlic cloves are prepared as sterile The polyploid callus is obtained by using the leaves and roots grown from the growth point of the sterile seedling to obtain the garlic polyploid. In the method of the invention, the bulb incision is provided with bulb tissue, and the bulb itself has nutritious tissue, which can be directly planted in the ground when the mutant is induced, and the survival rate is high; the incision of the garlic bulb soaked in colchicine can also be used to prepare sterile seedlings, Polyploid callus can be obtained from leaves and roots grown from the growth point of sterile seedlings, and garlic polyploids can be obtained without planting in the field.

Description

technical field [0001] The invention belongs to the technical field of garlic induction, in particular to a method for inducing garlic polyploidy or mutant. Background technique [0002] Polyploid breeding refers to the use of artificial mutagenesis or natural mutation, etc., to obtain polyploid breeding materials by doubling the genome of cells to select and breed excellent varieties that meet people's needs. Since the pioneering of ploidy breeding in 1937, there has been a global upsurge in polyploidy breeding. Polyploidy breeding is one of the common methods of crop breeding in modern times, and it has obvious advantages in improving the vegetative organs of vegetatively propagated crops. [0003] In recent years, great progress has been made in the polyploidy breeding technology of garlic. At present, the research on polyploidy of garlic has achieved the effect of chromosome doubling by using garlic stem tips, root tips and flower buds as materials and treated with col...

AI Technical Summary

Problems solved by technology

However, since the stripped shoot tips are easily necrotic after being treated with colchicine, the pollution rate is high and the survival rate is extremely low, which is the bottleneck that limits the induction of polyploidy from shoot tips; while other traditional garlic polyploid induction methods usually use treatment Callus, injection growth point and other technologies, but only aseptic operation, and the survival rate is not high, after the laboratory treatment, it needs to be transplanted to the field for treatment, it is difficult to guarantee a high survival rate

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