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Care solution for promoting skin regeneration and repair

A technology of skin regeneration and care solution, which is applied to skin diseases, medical preparations containing active ingredients, peptide/protein components, etc., to achieve the effects of promoting proliferation and cell migration, promoting skin regeneration and repair, and promoting skin repair

Active Publication Date: 2021-07-27
青岛市中医医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the known function of Halocyntin polypeptide is antibacterial, but there is no report about its effect in skin damage repair

Method used

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  • Care solution for promoting skin regeneration and repair
  • Care solution for promoting skin regeneration and repair
  • Care solution for promoting skin regeneration and repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] To detect whether Halocyntin polypeptide can promote the proliferation of human skin fibroblast HSF

[0029] (1) Add Halocyntin polypeptide to DMEM medium to make 1μM, 2.5μM, 5μM and 10μM, 20μM Halocyntin polypeptide solutions;

[0030] (2) Put 2×10 4 HSF cells were inoculated in a 96-well plate, and 0 μM, 1 μM, 2.5 μM, 5 μM and 10 μM Halocyntin polypeptide solutions were added respectively after adherence. After culturing for 72 hours, the 490nm OD value was detected by MTT. The obtained experimental results are as follows figure 1 and shown in Table 1.

[0031] Table 1 OD value of HSF cells treated with different concentrations of Halocyntin polypeptide solutions

[0032] Cell treatment concentration OD value 0μM 0.618±0.017 1μM 0.624±0.019 2.5μM 0.659±0.018 5μM 0.730±0.021 10μM 0.828±0.023 20μM 0.826±0.020

[0033] From figure 1 And in Table 1, it can be seen that 1 μM Halocyntin polypeptide has no obvious effec...

Embodiment 2

[0035] Detection of the effect of 10 μM Halocyntin polypeptide on the proliferation of human microvascular endothelial cells HMEC

[0036] (1) will be 2×10 4 HMEC cells were seeded in a 96-well plate, and 0 μM and 10 μM Halocyntin polypeptide solutions were added after attachment. After 24h, 48h, 72h, and 96h of culture, the 490nm OD value was detected by MTT. The experimental results obtained are as follows figure 2 and shown in Table 2.

[0037] Table 2 OD values ​​of HMEC cells treated with 10 μM Halocyntin peptide solution at different times

[0038] cell processing time 0μM 10μM 24h 0.218±0.011 0.246±0.013 48h 0.320±0.023 0.422±0.028 72h 0.542±0.031 0.758±0.036 96h 0.645±0.026 0.875±0.039

[0039] From Table 2 and figure 2 It can be seen from the figure that at 24h, 48h, 72h and 96h, the OD values ​​of the cells treated with 10μM Halocyntin polypeptide solution were significantly higher than those of the cells treated with Oμ...

Embodiment 3

[0041] Detection of the effect of 10 μM Halocyntin polypeptide on the cell migration ability of human skin fibroblasts

[0042] (1) Starve HSF cells with serum-free medium for 12 hours;

[0043] (2) After trypsinization, use serum-free DMEM medium containing 0 μM Halocyntin polypeptide and serum-free DMEM medium containing 0 μM Halocyntin polypeptide to resuspend the cells, and mix 1×10 5 Cells were seeded in the transwell upper chamber;

[0044] (3) Add DMEM medium containing 10% serum in the lower chamber of the transwell, and after culturing for 24 hours, take out the transwell chamber for staining, and after cleaning, select 5 different fields of view under the microscope for photographing and statistics. The experimental results are as follows image 3 shown.

[0045] It can be seen from the figure that the number of HSF cells passing through the transwell chamber after treatment with 10 μM Halocyntin polypeptide is significantly increased, and the effect is very signif...

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Abstract

The invention provides a care solution for promoting skin regeneration and repair, and belongs to the technical field of skin regeneration and repair. The core component of the care solution for promoting skin regeneration and repair provided by the invention is a Halocyntin polypeptide solution, and the content of Halocyntin polypeptide in the Halocyntin polypeptide solution is greater than or equal to 10 [mu]M. The care solution provided by the invention can effectively promote proliferation and migration of human skin fibroblasts and human microvascular endothelial cells, so that damage repair of skin can be effectively promoted.

Description

technical field [0001] The invention belongs to the technical field of skin regeneration and repair, and in particular relates to a care solution for promoting skin regeneration and repair. Background technique [0002] The skin is the organ with the largest surface area of ​​the human body, accounting for about 70% of the surface area of ​​the human body. The skin, which is a barrier protective tissue with a multi-layered structure, is interconnected with various systems of the human body. The skin has the functions of maintaining a relatively constant body temperature, protecting the body from the invasion of pathogenic bacteria, and maintaining the dynamic balance of electrolytes in the body. Since the skin is the body's protective tissue, it is susceptible to loss, common types of injuries include scalds, trauma, burns, etc. [0003] Skin injury repair is a hot and difficult point in biomedical research. The process of skin injury repair is a very complicated process. ...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P17/02
CPCA61K38/1767A61P17/02
Inventor 王纳宋海宁
Owner 青岛市中医医院
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