Preparation method of prunella vulgaris effective component for improving sleep and medicinal preparation

A technology for improving sleep and active ingredients, applied in the direction of medical preparations containing active ingredients, preparation of organic compounds, organic active ingredients, etc., can solve the problem of hindering the progress of rosmarinic acid and isorosmarinic acid, which has not been reported And other issues

Pending Publication Date: 2021-07-30
BEIJING UNIV OF CHINESE MEDICINE
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few studies on the extraction of the characteristic component isorosamarinic acid in Prunella vulgaris, and there is no report that extracting rosmarinic acid and isorismarinic acid as the material basis for improving sleep efficacy , which hinders the development of rosmarinic acid and isorosmarinic acid into new clinical drugs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of prunella vulgaris effective component for improving sleep and medicinal preparation
  • Preparation method of prunella vulgaris effective component for improving sleep and medicinal preparation
  • Preparation method of prunella vulgaris effective component for improving sleep and medicinal preparation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] The preparation method of the active ingredient of Prunella vulgaris for improving sleep of the present invention comprises the steps of extracting, enriching, purifying and further purifying.

[0047]

[0048] In the extracting step of Prunella vulgaris of the present invention, Prunella vulgaris is taken and extracted with a solvent to obtain Prunella vulgaris extract.

[0049] In the present invention, the extraction solvent is 30vol%-70vol% ethanol aqueous solution or water, preferably 30vol%-70vol% ethanol aqueous solution, more preferably 30vol% ethanol aqueous solution. The amount of solvent used is 5-20 times that of Prunella vulgaris, preferably 14-18 times, more preferably 15-17 times, and more preferably 16 times. According to a preferred embodiment of the present invention, 30 vol% ethanol aqueous solution with 16 times the amount of Prunella vulgaris is used as the extraction solvent. The 30vol% ethanol aqueous solution is used as the extraction solvent,...

Embodiment 1

[0076] The preparation method of the active ingredient of Prunella vulgaris for improving sleep of the present embodiment, the extraction steps include:

[0077] Take Prunella vulgaris, add 16 times the amount of 30vol% ethanol aqueous solution to it, reflux extraction at 100°C for 3 times, each time for 1.5h, filter, combine the filtrates, recover the solvent under reduced pressure, evaporate to dryness, and vacuum dry to obtain the Prunella vulgaris extract thing.

Embodiment 2

[0079] The preparation method of the active ingredient of Prunella vulgaris for improving sleep in this embodiment, the enrichment step includes:

[0080] Get the Prunella vulgaris extract prepared in Example 1, disperse it with water so that the solution concentration is 0.05g / mL, centrifuge at 4000r / min for 15min, get the supernatant as the sample solution, and the sample solution passes through the HPD-400 macroporous adsorption resin column, The loading sample amount of Prunella vulgaris extract and the resin volume ratio of HPD-400 macroporous adsorption resin column are 1:6g / mL, and resin volume is 24mL, and the diameter-to-height ratio of HPD-400 macroporous adsorption resin column is 1:10, First use 3BV of water to remove impurities, and then use 5BV of 30vol% ethanol aqueous solution to elute, collect the eluate, recover the solvent, evaporate to dryness, and dry in vacuum to obtain the eluate of Prunella vulgaris.

[0081] The above-mentioned HPD-400 macroporous adso...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a prunella vulgaris effective component for improving sleep and a medicinal preparation. The preparation method comprises the steps of extracting prunella vulgaris with a solvent to obtain a prunella vulgaris extract; enriching and eluting the prunella vulgaris extract through an HPD-400 macroporous adsorption resin column, collecting an eluent, and removing a solvent to obtain a prunella vulgaris eluate; putting the prunella vulgaris eluate on a silica gel column, averagely cutting the silica gel column into 10-30 sections from the column head to the bottom, respectively detecting and identifying each silica gel section by adopting a thin layer chromatography, respectively combining the silica gel sections containing rosmarinic acid or isorosmarinic acid glycoside, eluting with methanol, removing a solvent from the eluate, and drying to respectively obtain an effective part of rosmarinic acid and an effective part of isorosmarinic acid glycoside; and dissolving the effective part of the isorosmarinic acid glycoside and the effective part of the rosmarinic acid, and purifying by a C18 reverse silica gel column to respectively obtain an isorosmarinic acid glycoside monomer and a rosmarinic acid monomer. According to the method disclosed by the invention, the yield and the purity of the rosmarinic acid monomer and the isorosmarinic acid glycoside monomer are considered, and the steps are few.

Description

technical field [0001] The present invention relates to a preparation method and a pharmaceutical preparation of active ingredients of Prunella vulgaris for improving sleep, in particular to a preparation method for extracting rosmarinic acid monomers and isorosmarinic acid monomers from Prunella vulgaris, and containing rosmarinic acid monomers. Pharmaceutical preparations of marinic acid monomers and isosmarinic acid monomers. Background technique [0002] Prunella vulgarls (Prunella vulgarls L.) is the dry mature ear or whole plant of Prunella vulgarls L., which is cold in nature, bitter and pungent in taste, and returns to the liver and gallbladder meridian. Efficacy, used for conjunctival congestion and swelling, night carbuncle, headache, dizziness, arthralgia, cancer and breast swelling, goiter, lymph node tuberculosis, breast hyperplasia, hypertension and other diseases. The chemical composition of Prunella vulgaris is complex, mainly including flavonoids, triterpen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/56C07C69/732C07H1/08C07H15/203A61K31/216A61K31/7034A61P25/20
CPCC07C67/48C07C67/56C07H1/08C07H15/203A61K31/216A61K31/7034A61P25/20C07C69/732A61K2300/00
Inventor 程发峰姜艳艳王雪茜
Owner BEIJING UNIV OF CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap