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Culture medium for artificial propagation of jaffueliobryum wrightii and artificial propagation method

A culture medium and the technology of moss dendrites, which are applied in the field of culture medium for artificial propagation of moss dendrites and in the field of artificial propagation, can solve the problem of hindering the development of research on the biological characteristics of moss dendrites and the lack of artificial Propagation methods, inability to artificially propagate volcanic moss mosses, etc., to achieve the effect of artificial large-scale and rapid propagation, promoting growth, and solving a large number of needs

Pending Publication Date: 2021-08-06
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are artificial cultivation methods related to other types of bryophytes at present, there are significant differences in the growth characteristics and adaptability to the environment of different types of mosses, so the artificial cultivation methods for other types of bryophytes cannot be directly applied. Artificial propagation of the volcanic moss Physcomitrella syringae
Moreover, due to the difficulty in the gametophyte propagation of moss moss, there is still a lack of rapid and effective artificial propagation methods for the volcanic moss moss moss, which seriously hinders the development of research on the biological characteristics of moss moss

Method used

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  • Culture medium for artificial propagation of jaffueliobryum wrightii and artificial propagation method
  • Culture medium for artificial propagation of jaffueliobryum wrightii and artificial propagation method
  • Culture medium for artificial propagation of jaffueliobryum wrightii and artificial propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The growth status of the moss syringae gametophytes after adopting different disinfection methods in embodiment 1

[0042] 1. Test method

[0043] Under the dissecting microscope, pick out the moss gametophyte with better growth, wash away the soil attached to the surface, sonicate under ultrasonic for ten minutes, add an appropriate amount of detergent and ultrasonic for ten minutes again, rinse off the surface foam, and then wrap the moss gametophyte Bind it in gauze and place it under the faucet, rinse it with running water for 3-4 hours, and place the rinsed explants in an ultra-clean workbench for later use.

[0044] Prepare NaClO and HgCl respectively 2 and alcohol three different disinfection reagents, and set different concentration gradients and time gradients, the specific settings are as follows: the concentration of NaClO is set to 1% m / v, 1.5% m / v, 2.0% m / v, 2.5% m / v , the time gradient is set to 30s, 60s, 90s, 120s, 150s, 180s, a total of 24 groups, each...

Embodiment 2

[0053] The growth status of Physcomitrella syringae gametophytes in different basal mediums of embodiment 2

[0054] 1. Experimental method

[0055] Using 1.0% m / v HgCl 2 According to the disinfection method described in Example 1, the moss moss was disinfected for 90s; the moss moss explants after the disinfection treatment were respectively inoculated into Knop, improved Knop, MS, and 8g / L agar containing 15g / L sucrose, 1 / 2MS four kinds of sterilized and complete solid medium, mark the type of medium and the date of inoculation, seal it with a parafilm and place it in a light incubator for cultivation. Intensity 4000lx, 16h / 8h (continuous light for 16h every day, followed by continuous darkness for 8h). Observe the condition of explants turning green and growing protonema, record and take pictures.

[0056] 2. Experimental results

[0057] After cultivating for 15-20 days, the moss syringae explants inoculated on the Knop medium grow protonema at the shoot tip or the pla...

Embodiment 3

[0058] Embodiment 3 contains the growth status of the moss syringae gametophytes in the culture medium of different carbon sources

[0059] 1. Experimental method

[0060]Sucrose, glucose, and fructose were used as carbon sources respectively, and the Knop medium contained sucrose concentrations of 0g / L, 5g / L, 15g / L, 25g / L, 35g / L, and 45g / L respectively; Knop medium contained Glucose concentrations were 0g / L, 10g / L, 20g / L, 30g / L, 40g / L, 50g / L; the concentrations of fructose in Knop medium were 0g / L, 2g / L, 4g / L, 6g / L, 8g / L, 10g / L; using 1.0% m / v HgCl 2 According to the disinfection method described in Example 1, the moss moss was disinfected for 90 seconds; the moss moss explants after the disinfection treatment were respectively inoculated into the knop medium containing the above-mentioned carbon source, and the type and date of inoculation were marked , sealed with a parafilm and placed in a light incubator for cultivation under the conditions of temperature 24°C, light in...

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Abstract

The invention belongs to the technical field of plant propagation culture, and particularly relates to a culture medium for artificial propagation of jaffueliobryum wrightii and an artificial propagation method. Firstly, the culture medium for artificial propagation of the jaffueliobryum wrightii is provided, and the culture medium is prepared by taking a Knop culture medium as a basic culture medium and adding 20-40 g / L of glucose, 0.5-2.5 mg / L of IBA and 8-10 g / L of agar; and secondly, the artificial propagation method of the jaffueliobryum wrightii is provided, and the method comprises the following steps: (1) treating the surface of jaffueliobryum wrightii explants by using 0.05-0.1% of HgCl2; and (2) inoculating the disinfected jaffueliobryum wrightii explants on the culture medium, and culturing the jaffueliobryum wrightii explants. According to the method, the blank in the technical field of propagation of the gametophyte of the jaffueliobryum wrightii in the prior art is filled, the artificial mass rapid propagation of the gametophyte of the jaffueliobryum wrightii is realized, and a foundation is laid for the biological characteristic research of the jaffueliobryum wrightii.

Description

technical field [0001] The invention belongs to the technical field of plant multiplication and cultivation, and in particular relates to a culture medium for artificial multiplication of moss syringae and an artificial multiplication method. Background technique [0002] The plants of bryophytes are small, and most of the leaves are single-layer cells without differentiation to form fibrous tube tissue, which makes it difficult to cultivate gametocytes in bryophytes in vitro. Most of the research on tissue culture of bryophytes at home and abroad is collected in large quantities in the wild or using sporophytes as explants for artificial in vitro propagation. However, a large number of field excavations will seriously damage the natural and ecological environment, and the collection cost is high; spore propagation Due to the influence of environmental and seasonal factors, such problems as difficult spore collection and low spore germination rate are prominent, and it is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵东平杨倩王丽红王丹
Owner INNER MONGOLIA UNIVERSITY
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