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Double-targeting composite nano-system loaded with amphotericin B and beta-1,3-glucanase, and preparation method and application of double-targeting composite nano-system

A technology of glucanase and amphotericin, applied in biochemical equipment and methods, glycosylase, medical preparations of non-active ingredients, etc., can solve problems such as superbug infection and rare Candida albicans biofilm , to achieve the effect of improving the scavenging ability

Pending Publication Date: 2021-08-06
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But most work has only focused on single bacterial biofilms, and studies on C. albicans biofilms are extremely rare
At present, the use of excessive chemical drugs (antibiotics), leading to infections of drug-resistant bacteria and even superbugs, has become an urgent problem to be solved

Method used

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  • Double-targeting composite nano-system loaded with amphotericin B and beta-1,3-glucanase, and preparation method and application of double-targeting composite nano-system
  • Double-targeting composite nano-system loaded with amphotericin B and beta-1,3-glucanase, and preparation method and application of double-targeting composite nano-system
  • Double-targeting composite nano-system loaded with amphotericin B and beta-1,3-glucanase, and preparation method and application of double-targeting composite nano-system

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Embodiment 1

[0037] (1) Preparation of chitosan nanoparticles (CSNP) by ion gelation method using polyanion sodium triphosphate (TPP): TPP (5 mg / ml) dissolved in distilled water was added dropwise to chitosan solution (5 mg / ml mL, dissolved in 1% (v / v) acetic acid, 10 mL), mass ratio CS:TPP = 4:1, the mixture was stirred for 2 hours; finally, nanoparticles were obtained by centrifugation at 14,000 rpm for 30 minutes.

[0038] (2) Preparation of Gls-loaded chitosan nanoparticles (CSNP-Gls), while mixing CSNP (1 mg / ml PBS) and Gls (100 μg / ml PBS), stirring overnight at 4 °C. The NP suspension was then centrifuged (14000 rpm, 30 min) and finally freeze-dried.

[0039] (3) Chitosan nanoparticles loaded with Gls and AmpB (CSNP-AmpB-Gls), CSNP-Gls suspension (1 mg / ml) was mixed with AmpB (100 μg / ml DMSO) and stirred for 24 hours, the mixture was centrifuged and lyophilized.

[0040] (1) Characterization of nanoparticles

[0041] The size of the nanoparticles was analyzed by dynamic light scat...

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Abstract

The invention belongs to the technical field of nano-drugs, and particularly relates to a double-targeting composite nano-system loaded with amphotericin B (AmpB) and beta-1,3-glucanase (Gls), and a preparation method and application of the double-targeting composite nano-system. According to the nano-system provided by the invention, Gls and AmpB are loaded by chitosan nanoparticles. The beta-1,3-glucanase modified chitosan nanoparticles are constructed for the first time, a double-targeting nano-system for fungal biofilm exopolysaccharide beta-1,3-glucan and internal thalli can be realized, and meanwhile, the beta-1,3-glucanase and the amphotericin B have a synergistic interaction effect, so that the removal capacity is improved. The double-loaded nano-system is used for damage removal of a fungal biofilm for the first time.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a double-targeting composite nanosystem loaded with amphotericin B and β-1,3-glucanase, a preparation method and application thereof. Background technique [0002] Candida albicans is one of the main food-borne pathogens, causing frequent food safety incidents and even causing death of patients. According to statistics, invasive candidiasis has become the main cause of fungal-related disease mortality, and its mortality rate exceeds 70%. Biofilm is the main form of Candida albicans, that is, fungal cells adhere to the contact surface, produce a large amount of extracellular matrix (mainly polysaccharides) by itself, and form a microbial aggregation structure that surrounds itself. The extracellular matrix not only participates in the formation of the biofilm structure, but also protects the internal microorganisms from the harsh external environment, and signifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K47/69A61K47/36A61K47/64A61K38/47A61K31/7048A61P31/10
CPCA61K31/7048A61K38/47A61K9/19A61K47/36A61K47/64A61K47/6939C12Y302/01006A61P31/10A61K2300/00Y02A50/30C12Y302/01058A01P1/00A01N43/90A01N63/50A01N25/14A01N25/10A01N25/12
Inventor 谭玉龙马素
Owner QINGDAO AGRI UNIV
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