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Preparation method of natural biofilm carrier for surfactant wastewater treatment

A biofilm carrier and surfactant technology, which is applied in the field of preparation of natural biofilm carriers, can solve problems such as microplastic pollution, achieve fast film-hanging speed, high environmental friendliness, and improve the effect of film-hanging efficiency

Active Publication Date: 2021-08-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method of the present invention has the advantages of simple operation, high economy and environmental friendliness, and the natural biofilm carrier prepared by it has biodegradability, which can solve the microplastic pollution problem existing in the existing synthetic polymer biofilm carrier

Method used

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  • Preparation method of natural biofilm carrier for surfactant wastewater treatment
  • Preparation method of natural biofilm carrier for surfactant wastewater treatment
  • Preparation method of natural biofilm carrier for surfactant wastewater treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Gelatin was extracted from waste leather scraps by alkaline hydrolysis, and 5 g of gelatin was added to 50 ml of phosphate buffer (pH=7.5), stirred in a water bath at 50 °C for 30 min to obtain a homogeneous gelatin aqueous solution. Then 0.2 g of methacrylic anhydride was added to it, and the reaction time was 3 h in a water bath at 50 °C. After the reaction, 100 ml of phosphate buffer was added to dilute to terminate the reaction, and the obtained product was used in a 40 °C constant temperature incubator. Dialyzed with deionized water for 3 days and freeze-dried to prepare methacrylylated modified gelatin containing carbon-carbon double bonds (C=C).

[0028] Prepare 20 ml of methacrylylated modified gelatin aqueous solution with a concentration of 2.5 % w / v, add 0.03 g of photoinitiator to it, and then freeze the mixed solution at -18 ℃ for 16 h; then freeze the frozen system Irradiated under ultraviolet light, the light intensity was 50 mw / cm2, and the irradiation t...

Embodiment 2

[0030] Gelatin was extracted from waste leather scraps by alkaline hydrolysis, and 5 g of gelatin was added to 50 ml of phosphate buffer (pH=7.5), stirred in a water bath at 50 °C for 30 min to obtain a homogeneous gelatin aqueous solution. Then 0.2 g of methacrylic anhydride was added to it, and the reaction time was 3 h in a water bath at 50 °C. After the reaction, 100 ml of phosphate buffer was added to dilute to terminate the reaction, and the obtained product was used in a 40 °C constant temperature incubator. Dialyzed with deionized water for 3 days and freeze-dried to prepare methacryloyl-modified gelatin containing carbon-carbon double bonds (C=C).

[0031] Prepare 20 ml of methacrylylated modified gelatin aqueous solution with a concentration of 2.5 % w / v, add 0.03 g of photoinitiator to it, and then freeze the mixed solution at -18 °C for 16 h; then freeze the frozen system Irradiated under ultraviolet light, the light intensity was 50 mw / cm2, and the irradiation tim...

Embodiment 3

[0033] Gelatin was extracted from waste leather scraps by alkaline hydrolysis, and 5 g of gelatin was added to 50 ml of phosphate buffer (pH=7.5), stirred in a water bath at 50 °C for 30 min to obtain a homogeneous gelatin aqueous solution. Then 0.4 g of methacrylic anhydride was added to it, and the reaction time was 3 h under the condition of 50 °C water bath. After the reaction was completed, 100 ml of phosphate buffer was added to dilute to terminate the reaction. Dialyzed with deionized water for 3 days and freeze-dried to prepare methacryloyl-modified gelatin containing carbon-carbon double bonds (C=C).

[0034]Prepare 20 ml of methacrylylated modified gelatin aqueous solution with a concentration of 2.5 % w / v, add 0.03 g of photoinitiator to it, and then freeze the mixed solution at -18 ℃ for 16 h; then freeze the frozen system Irradiated under ultraviolet light, the light intensity was 50 mw / cm2, and the irradiation time was 5 min to obtain the all-biomass-based gel; f...

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Abstract

The invention provides a preparation method of a natural biofilm carrier for surfactant wastewater treatment. The method mainly comprises the following steps: (1) firstly, extracting gelatin from waste leather leftover materials through alkaline hydrolysis, and then modifying the gelatin by utilizing methacrylic anhydride to prepare modified gelatin with carbon-carbon double bonds on a molecular chain; and (2) preparing the natural biofilm carrier by using the modified gelatin in the step (1) as a raw material through a freezing-ultraviolet radiation polymerization method. The method provided by the invention has the advantages of simple process, high economy and environmental friendliness. The biofilm carrier prepared by the method is a porous material prepared by taking natural biomass as a raw material, so that the problem of micro-plastic pollution caused by synthesizing a polymer biofilm carrier can be avoided; and the structure of the biofilm carrier contains an-glyci argininene-aspartic acid (RGD) sequence capable of promoting microbial adhesion, so that the biofilm carrier has unique advantages as a biofilm carrier and has a good market application prospect.

Description

technical field [0001] The invention relates to the technical field of surfactant wastewater treatment and preparation of natural biofilm carriers, in particular to a preparation method of natural biofilm carriers used for surfactant wastewater treatment. Background technique [0002] As an industrial additive, surfactant is widely used in various industries of the national economy, but the surfactant wastewater produced at the same time also brings ecological and environmental problems. Surfactants are listed as the second type of pollutants in my country, and according to the discharge standards of urban sewage treatment plants in my country, the highest discharge standard is 5 mg / L. Therefore, surfactant wastewater must be effectively treated before it is discharged into the natural environment. The biofilm method is one of the main methods of surfactant wastewater treatment, and its core is to provide a carrier for microorganisms to adhere to, grow and reproduce. [00...

Claims

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Application Information

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IPC IPC(8): C02F3/00B01J13/00C02F103/34
CPCC02F3/00B01J13/0052B01J13/0065B01J13/0069C02F2103/34Y02W10/10
Inventor 林炜何雨静王春华王帅
Owner SICHUAN UNIV
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