Oral health model for high throughput screen and characterization of oral hygiene products

A model, oral technology, applied in the field of oral health models for high-throughput screening and characterization of oral hygiene products, capable of solving problems such as destruction of the bones and tissues that support teeth

Inactive Publication Date: 2021-08-06
COLGATE PALMOLIVE CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] As an unfortunate consequence, these elements inadvertently destroy the bone and tissue that support the teeth

Method used

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  • Oral health model for high throughput screen and characterization of oral hygiene products
  • Oral health model for high throughput screen and characterization of oral hygiene products
  • Oral health model for high throughput screen and characterization of oral hygiene products

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] The preparation of each component of the GCM is coordinated such that each component of the GCM is ready for testing at the same time. Epithelial tissue must be obtained, HL60 cells must be induced with retinoic acid to differentiate into a neutrophil-like phenotype (PMN), and biofilms must be prepared. The preparation of PMNs and biofilms was coordinated such that PMNs and biofilms were ready after receipt from the supplier and overnight incubation of the MatTek tissue. After delivery, MatTek tissues (epithelial cells) were placed in fresh medium in 6-well plates and allowed to recover overnight in an incubator. GCMs were then assembled using cells and biofilms induced into a neutrophil-like phenotype (PMN).

[0027] GCM can be used to test various test compositions, such as mouthwash or toothpaste. When testing toothpaste (TP) in GCM, the product is prepared as a slurry. Immediately prior to the test, use ultrapure H at a 1:2 dilution 2 O dilutes the TP product. ...

example 1

[0043] GCM was used to evaluate toothpaste compositions, including Composition 1 (Comp1), Composition 2 (Comp2) and Composition 3 (Comp3). Composition 1 comprises zinc oxide, zinc citrate and stannous fluoride. Zinc oxide is present in the composition at about 1%. Zinc citrate is present in the composition at about 0.5%. Stannous fluoride is present in the composition at about 0.5%. Composition 2 comprises zinc oxide, zinc citrate, stannous fluoride and arginine. Zinc oxide is present in the composition at about 1%. Zinc citrate is present in the composition at about 0.5%. Stannous fluoride is present in the composition at about 0.5%. Arginine is present in the composition at about 1.5%. Composition 3 comprises zinc oxide, zinc citrate, stannous fluoride and zingerone. Zinc oxide is present in the composition at about 1%. Zinc citrate is present in the composition at about 0.5%. Stannous fluoride is present in the composition at about 0.5%. Zingerone is present in th...

example 2

[0048] GCM was used to evaluate a toothpaste composition comprising zinc oxide, zinc citrate and arginine, Composition 4 (Comp4). Zinc oxide is present in the composition at about 1%. Zinc citrate is present in the composition at about 0.5%. Arginine is present in the composition at about 1.5%. The data from the evaluation is in Figure 8 and shown in Table 1.

[0049] GCM was used to see the effect on the bacterial community. Specifically, experiments were performed to evaluate how treatment of toothpaste compositions including Composition 4 affected the microbiome. Two different biofilms were obtained, one from a healthy person and one from a healthy person with gum disease. Figure 8 A comparison of the composition of the two biofilms is shown. Biofilm "A" represents a biofilm from a healthy person with gum disease. Biofilm "B" indicates a biofilm from a healthy person who did not suffer from gum disease. Dashed boxes correspond to the percentages of different patho...

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Abstract

An oral biology model which comprises biofilm, oral epithelial tissue and a suspension of neutrophil-like cells in media is disclosed. The biofilm, which comprises oral bacteria, is produced by culturing oral bacteria on a solid substrate. The oral epithelial tissue may be gingival epithelial tissue to model the gingival crevice or buccal epithelial tissue to model the oral check. The suspension of neutrophil-like cells in media comprises neutrophil-like cells that are differentiated HL60 cells induced to a neutrophil-like phenotype by treatment with retinoic acid. Methods of using the oral biology model to test and compare compounds and formulations or to screen compounds and formulations for their effect on release of inflammatory signals, their effect on biofilm and oral bacteria and / or their effect on the cellular components are disclosed.

Description

Background technique [0001] Gums, also known as gums, are the part of the soft tissue lining of the mouth that surrounds and provides a seal around teeth. The gingival margin is the interface between the gingival sulcus epithelium and the oral epithelium. This interface exists at the strongest coronal point of the gingiva, also known as the coronal portion of the marginal gingiva. The gingival crevice, also known as the gingival sulcus, is the space surrounding a tooth between the wall of unattached gum tissue and the enamel and / or cementum of the tooth. [0002] Gum disease is an inflammation of the gum line that can progress to affect the bone that surrounds and supports your teeth. The three stages of gum disease -- from mildest to most severe -- are gingivitis, periodontitis, and advanced periodontitis. Gingivitis is the initial stage of gum disease. The immediate cause of gingivitis is plaque -- the soft, sticky, colorless film of bacteria that continually forms on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0697C12N5/0632C12N2502/1135C12N2503/04C12N2500/72C12N2500/22C12N2533/54C12N2533/32C12R2001/04C12R2001/46A61K8/27A61Q11/00C12N1/20C12N2513/00C12N2533/18G01N33/5008G09B23/306
Inventor 卡米耶·泽诺比娅哈什·马亨德拉·特里维迪杨英卡洛·德埃普詹姆斯·马斯特斯
Owner COLGATE PALMOLIVE CO
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