132 results about "Retionic Acid" patented technology

The present invention relates to a method for preparing organ-specific mesenchymal cells from pluripotent stem cell-derived intestinal organoid stromal cells. By using cells derived from stromal cell layers adjacent to intestinal organoids for differentiation into organ-specific mesenchymal cells, the present invention can greatly increase the efficiency of differentiation into stromal cells through the regulation of retinoic acid (RA) and hedgehog (HH) signaling pathways, and can increase the expression of organ-specific markers without exhibiting undifferentiated state cell characteristics, and thus mesenchymal cells having well-simulated biological characteristics can be prepared.

The invention discloses retinol retinoate-ceramide lipidosome modified by hyaluronic acid and salts thereof and a preparation method of the retinol retinoate-ceramide lipidosome, and belongs to the technical field of cosmetic nano-carriers. The preparation method comprises the steps that retinol retinoate-ceramide lipidosome is prepared, hyaluronic acid is pretreated, and the hyaluronic acid and the salts thereof are combined to prepare the retinol retinoate-ceramide lipidosome. The liposome is modified by hyaluronic acid and salts thereof in a dual-mechanism manner, and purification and stabilization are realized; the preparation method of the retinol retinoate-ceramide liposome comprises the following steps: dissolving phospholipid, a functionalized lipid component with positive ions and amino functional groups, cholesterol, retinol retinoate and ceramide in absolute ethyl alcohol to serve as an organic phase, taking a phosphate buffer solution as a water phase, dropwise adding the organic phase into the water phase under a stirring condition, stirring, performing ultrasonic treatment, washing, and drying to obtain the retinol retinoate-ceramide liposome. Performing membrane extrusion to obtain a liposome suspension; the retinol retinoate-ceramide liposome modified by hyaluronic acid and salts thereof prepared by the invention has excellent stability, skin transmittance and anti-aging and moisturizing effects, also has low irritation, and is suitable for anti-aging cosmetics.

The invention discloses a spot-fading, whitening and skin-tightening composition and a preparation method thereof, and particularly belongs to the technical field of cosmetics. The composition is prepared from an extract of bark of the cissampelos maculata, hydroxyl pinacolone 9-cis-retinoate, sclareolide, tetrahydromagnolol and a quercetin dimer. The composition provided by the invention can significantly reduce the skin melanin content, improve the skin brightness and significantly improve the skin elasticity and compactness, all the components have a synergistic effect, the effects of spot fading, whitening and skin tightening are effectively achieved, the preparation process provided by the invention is stable and controllable, and the composition is suitable for large-scale production and has wide application prospects. A novel efficient skin care composition and an application scheme are provided for the field of cosmetics.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

In a method for inducing differentiation into neuromuscular junctions according to the present disclosure, the neuromuscular junctions are induced by: preculturing human pluripotent stem cells in a medium containing activin, bFGF, a WNT signal inhibitor, a BMP signal inhibitor, and a ROCK signal inhibitor; then culturing the stem cells in a medium containing bFGF, a ROCK signal inhibitor, a WNT signal activator, and a TGFβ signal inhibitor to differentiate into neuromesodermal cells; culturing the neuromesodermal cells in a medium containing a BMP signal inhibitor, a TGFβ signal inhibitor, a retinoic acid activator, and an SHH signal activator to differentiate into skeletal muscle progenitor cells and spinal motor neuron progenitor cells in the same culture dish; and culturing these progenitor cells in a medium for cell maturation containing BDNF, GDNF, AA, and dbcAMP.

The invention discloses an agonist of a retinoic acid receptor beta and application of the agonist in preparation of a non-small cell lung cancer medicine in the technical field of biological medicine. The agonist of the retinoic acid receptor beta is saturated odd fatty acid heptadecanoic acid C17: 0; according to the research, the expression of a retinoic acid receptor beta can be up-regulated by adding heptadecanoic acid into the NSCLC cells, so that the sensitivity of the NSCLC cells to retinoic acid treatment is improved. When heptadecanoic acid and retinoic acid are combined for use, the activity and migration ability of NSCLC cells can be further inhibited, and apoptosis of the cells is promoted. The result shows that the heptadecanoic acid plays an important role in controlling the sensitivity of the NSCLC cells to the retinoic acid, the combined use of the heptadecanoic acid and the retinoic acid provides a new drug treatment means for treating the NSCLC, and the heptadecanoic acid and the retinoic acid have potential application value in preparing the drug for treating the non-small cell lung cancer.

The invention provides efficient mild whitening cream and a preparation method thereof. The raw materials of the cream comprise five phases A and E, the phase C contains 0.1-3% of a mixed solution of isosorbide dimethyl ether and hydroxyl pinacolone retinoate, and the phase D contains 0.01-0.1% of L-carnitine. Wherein the hydroxyl pinacolone retinoate can be specifically combined with an RAR / RXR (Region Receptor / Receptor X Receptor), and the expression of an MITF (Microphthalmia-associated Transcription Factor) gene is reduced, so that the melanogenesis is inhibited; l-carnitine promotes fatty acid beta-oxidation and enhances ATP synthesis to indirectly regulate and control AMPK activity; aMPK enhances transcriptional activity of a tretinoin receptor through phosphorylation of the tretinoin receptor, so that the AMPK and hydroxyl pinacolone retinoate exert a synergistic effect, jointly enhance whitening activity, effectively improve the phenomena of color spots and dark skin and promote the skin color to be uniform, bright and white; meanwhile, the isosorbide dimethyl ether can promote the transdermal absorption of the hydroxyl pinacolone retinoate, the L-carnitine and the related active ingredients.

The main purpose set by the present inventors is to provide a technology for suppressing inflammation particularly in aged tissue in the oral cavity.　The present inventors have discovered that it is possible to suppress inflammation particularly in aged tissue in the oral cavity by an oral cavity composition that contains β-glycyrrhetinic acid and at least one selected from the group consisting of retinol acetate, retinol, all-trans-retinoic acid, ascorbic acid, cobalamin, calciferol, anthraquinone, L-histidine, methylhesperidin, and chemically possible salts thereof.

The technology described herein is directed to modulate the levels of CD8+ T cells in the colon and / or small intestine including administering to a subject in need thereof a therapeutically effective amount of a retinoic acid receptor (RAR) agonist or inhibitor and / or an aryl hydrocarbon receptor (AHR) agonist or inhibitor, and a therapeutic agent.

The present invention belongs to the technical field of cosmetics, and relates to an organosilicon compound derived from ascorbic acid or retinoic acid, and a preparation method therefor and the use thereof. The ascorbic-acid-derived organosilicon compound prepared from ascorbic acid in the present invention shows higher cell proliferation-promoting activity on human immortalized keratinocyte cell line HaCaT and better skin anti-aging activity while maintaining anti-oxidant activity, and has good application potential in improving skin condition. Compared with retinoic acid, retinyl retinoate and pinacolone retinoate, the retinoic-acid-derived organosilicon compound prepared from retinoic acid has better proliferation-promoting activity on fibroblasts and human immortalized keratinocyte cell line HaCaT, has good light / thermal stability, potentially better transdermal absorption and causes less skin irritation, and has great development prospects.

The present disclosure provides the use of ATDR and ATDRA in the treatment of white matter diseases, and in particular provides all trans-13, 14-dihydroretinol (ATDR) and / or all trans-13, 14-dihydroretinoic acid (ATDRA) or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable salt comprising all trans-13, 14-dihydroretinol (ATDR) and / or all trans-13, 14-dihydroretinoic acid (ATDRA) or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable salt comprising all trans-13, 14-dihydroretinoic acid (ATDRA) and / or all trans-13, 14-dihydroretinoic acid (ATDRA) or a pharmaceutically acceptable salt thereof. The present invention relates to a composition comprising 1, 14-dihydroretinoic acid (ATDRA) or a pharmaceutically acceptable salt thereof, retinol saturase (Retsat) or a variant thereof, a nucleic acid encoding Retsat or a variant thereof, an expression cassette comprising a nucleic acid encoding Retsat or a variant thereof, a vector comprising a nucleic acid encoding Retsat or a variant thereof, and a method for preparing the same. Use of a cell expressing Retsat or a variant thereof for the preparation of a medicament for the prevention and / or treatment of leukoencephalopathy in a subject in need thereof, which promotes oligodendrocyte differentiation and myelination to thereby treat leukoencephalopathy.

The invention discloses a composition containing an exosome loaded with hydroxyl pinacolone 9-cis-retinoate and application of the composition, and belongs to the technical field of cosmetic raw materials. The core components of the composition are the agilawood exosome loaded with hydroxyl pinacolone 9-cis-retinoate and the fibroin, and the composition forms a synergistic effect through the efficient delivery characteristic of the agilawood exosome and the film-forming moisturizing effect of the fibroin, so that the problems that the traditional retinol component is unstable, the skin permeability is poor and the irritation is strong are solved, and the composition has a good application prospect. The defects that in an existing exosome delivery technology, the compatibility of active ingredients is poor, and the exosome is prone to inactivation and difficult to reside on the skin surface are overcome, the deep permeation, slow release and targeting effects of hydroxypinacolone 9-cis-retinoate can be achieved, the tightening, anti-wrinkle, whitening and spot fading effects are remarkably improved, and skin irritation is reduced. Experiments prove that the tightening and anti-wrinkle essence prepared on the basis of the composition has an excellent skin improving effect.

The invention discloses a method for efficiently and directionally inducing IPS to be differentiated into pancreatic beta cells. According to the method, through staged signal regulation and strict cell screening, the directionality and the overall efficiency of differentiation are remarkably improved. The whole process pays attention to cell quality control from the initial stage, and a stable foundation is laid for subsequent differentiation by screening iPS cells with uniform morphology and high expression of pluripotent markers. Progressive signal molecule regulation is adopted in each stage, and an accurate differentiation path is formed from Activin A gradient adjustment during endoderm induction, synergistic effect of FGF10 and retinoic acid in pancreatic precursor cell differentiation and Wnt signal inhibition during endocrine precursor cell induction, so that the non-specific differentiation tendency is effectively reduced. Meanwhile, cell enrichment steps of key nodes, such as SOX17 positive cell screening in an endoderm stage and Ngn3 magnetic bead sorting of endocrine precursor cells, further improve the purity of a target cell population and avoid interference of parenchyma cells on subsequent function maturation.

The present application is directed to stabilized core-shell microcapsules comprising a core of benzoyl peroxide (BPO) or all trans retinoic acid (ATRA) and a metal-oxide shell; and to pharmaceutical compositions and methods of use thereof.

The invention discloses a whitening and brightening composition and a cosmetic thereof. The whitening and brightening composition is prepared from ascorbyl tetraisopalmitate, tranexamic acid, Brigtenyl, LightSKN CN, a resveratrol fermentation product, fermented lotus essence and super vitamin A, brigtenyl is a mixture composed of a first solvent, glycerin and diglucosyl gallic acid; lightSKN CN is a mixture composed of a second solvent, 1, 3-propylene glycol, xanthophyll, lecithin, sodium ascorbate and vitamin E; the resveratrol fermentation product is a mixture composed of a third solvent, a pichia pastoris fermentation lysate filtrate and resveratrol; the fermented lotus seed essence is a mixture composed of a fourth solvent, butanediol and a lotus seed extract; the super vitamin A is a mixture of isosorbide dimethyl ether and hydroxyl pinacolone retinoate. The whitening and brightening composition not only can inhibit melanin generation, reduce melanin transfer and accelerate melanin metabolism, but also can remove excessive free radicals in skin, so that the effects of long-acting skin whitening and skin brightening are achieved.

The present invention relates to a composition comprising sustained release particles of hydroxy pinnacyl retinoate in which the ratio of hydroxy pinnacyl retinoate : methyl methacrylate / ethylene glycol dimethacrylate crosspolymer : ascorbyl tetraisopalmitate or tocopherol is from 0.05 to 3 : 0.1 to 10 : 0.05 to 1 and the use thereof in skin care.

The present invention relates to a method for producing neuronal cells from dedifferentiated adipocytes derived from a mammal, the method comprising a step for culturing dedifferentiated adipocytes derived from a mammal using a medium that contains a basic fibroblast growth factor (bFGF), retinoic acid, and a neuronal differentiation inducer, but does not substantially contain an epigenetic inhibitor.

This invention discloses a multi-target anti-aging composition and its application in cosmetics. By weight, it comprises the following ingredients: 0.001–3 parts hydroxypinazone retinate, 0.01–5 parts hydroxypropyl tetrahydropyranotriol, 0.01–0.5 parts recombinant human collagen, 0.5–3 parts tocopheryl acetate, 0.1–5 parts glyceryl glucoside, 0.01–5 parts Babao Essence Extract, 0.01–0.5 parts carnosine, 0.5–2 parts 3000 peptides, 0.1–5 parts Bifida ferment lysate, 0.01–0.5 parts coenzyme Q10, and 0.1–5 parts black tea fermentation product. This multi-target anti-aging composition targets four aspects: anti-aging, anti-oxidation, anti-glycation, and anti-photochemical activity. The four categories of components work synergistically to significantly enhance the anti-aging efficacy of the product. Human efficacy evaluation tests show that after using the product of this invention for 28 days, the elasticity of the skin around the eyes is significantly increased, the firmness and roughness of the skin around the eyes are significantly improved, and the area, volume and depth of wrinkles at the corners of the eyes are significantly improved.

The utility model discloses a recrystallization device for synthesizing hydroxyl pinacolone retinoate, which is characterized in that a filter base, a conical filter plate of the filter base and an annular scraper are arranged at the bottom of a kettle body, so that the filter efficiency is improved, and the yield is increased; the discharging device is matched with the filtering base, and the discharging device and the filtering base are matched for use, so that the efficiency can be improved while the yield is improved, and the operation is simple.

The invention discloses a method for synthesizing retinoic acid ceramide ester through enzyme catalysis and application of the retinoic acid ceramide ester, and belongs to the technical field of cosmetic raw material synthesis. According to the method, retinoic acid and ceramide NP serve as substrates, specific immobilized lipase serves as a catalyst, and in a non-aqueous phase organic solvent, the target product is efficiently synthesized through the steps of raw material preparation, anaerobic reaction system building, directional esterification reaction, aftertreatment and purification detection. The method solves the problems of low substrate solubility, more byproducts, difficulty in catalyst recovery and the like in the traditional process, has the advantages of mild reaction conditions, capability of retaining the activity of the raw materials, high product purity and anti-aging and barrier repair effects, and can be widely used for preparing raw materials of anti-wrinkle and repair cosmetics.

The present invention relates to a process for producing a population of cells which comprises mature atrial cardiomyocytes. The process comprises the step of treating iPS cells according to a treatment regimen which comprises contacting the iPS cells with Gremlin2 and retinoic acid, such that at least a portion of the iPS cells differentiate into mature atrial cardiomyocytes.

The present disclosure relates to a method for predicting the response of a metastasis to immunotherapy in a metastatic colorectal cancer patient. The present disclosure also encompasses an immunotherapy for use in the treatment of a metastatic colorectal cancer in a patient in need thereof previously identified as having a metastasis responsive to immunotherapy and an immune-stimulating agent for use in the treatment of a metastatic colorectal cancer in a patient in need thereof previously identified as having a metastasis non-responsive to immunotherapy. The present disclosure also relates to a pharmaceutical composition comprising an immune-stimulating agent and retinoic acid for use in the treatment of a metastatic colorectal cancer in a patient in need thereof.

A water-in-oil emulsion with an aqueous and an oil phase, such that the oil phase comprises at least one oil-soluble sunscreen, a functional elastomer, a retinoic acid precursor and a substituted resorcinol.

An oil-in-water emulsion having an SPF of at least 25, comprising: (a) at least 0.01 wt.% of one or more types of retinol selected from the group consisting of retinol, retinaldehyde, retinoic acid, or a combination thereof; (b) one or more water soluble antioxidants; (c) one or more oil soluble antioxidants; (d) at least 10 wt.% of one or more oil soluble or oil dispersible sunscreen components. Methods of making the oil-in-water emulsions as well as uses and methods of use of the oil-in-water emulsions.

Provided herein are methods, compositions and kits to enhance anti-cancer T-cell therapy. In particular, provided herein are reagents and methodologies to promote anti-cancer T-cell therapy comprising inhibition of retinoic acid receptor alpha (RARα) in chimeric antigen receptor (CAR) T cells.

The invention discloses application of a bifidobacterium longum subsp. Infantis CCFM1426 strain and vitamin A in preparation of a medicine for improving the physiological activity of the vitamin A and / or relieving colitis and / or xerophthalmia symptoms. The probiotic agent provided by the invention comprises a bifidobacterium longum subsp. Infantis CCFM1426 strain with the preservation number of GDMCC No.65152 and vitamin A, and can be used for improving the retinoic acid level in the colon of a mouse with ulcerative colitis; the diamine oxidase level, the intestinal fatty acid binding protein level and the lipopolysaccharide binding level in serum of mice with ulcerative colitis are reduced; the corneal injury, basal vacuolation and conjunctival goblet cell injury of the xerophthalmia mice are relieved; the level of retinoic acid in serum of the dry-eye mouse is improved; and the levels of IL-6, IL-17 and TNF-alpha in serum of dry eye mice are reduced. Therefore, the probiotic agent disclosed by the invention has a relatively great application prospect in preparation of products for supplementing vitamin A and preventing and / or treating ulcerative colitis and xerophthalmia.

The invention belongs to the technical field of cell biology, and particularly relates to a culture medium for amplifying Treg cells, a culture method and application. The culture medium comprises an activation culture medium and an amplification culture medium, wherein the activated culture medium is prepared from a serum-free culture medium, IL-2 (Interleukin-2), TGF (Transforming Growth Factor)-beta (beta), rapamycin, IL-27 (Interleukin-27), retinoic acid and Anti-Human CD3 / CD28 beads; the amplification culture medium comprises a serum-free culture medium, IL-2, rapamycin and IL-27. According to the invention, the culture medium combination with a specific formula and proportion is adopted, and the synergistic effect of the IL-2, the TGF-beta, the rapamycin, the IL-27, the retinoic acid and the Anti-Human CD3 / CD28 beads is utilized, so that the amplification multiple and the immunosuppression capability of the Treg cells are remarkably improved. The invention provides a more efficient and safer technical scheme for clinical application of Treg cell therapy.

The present invention relates to compounds of formula I:in which A1-A7 and R1 to R5 are defined herein, for use in the treatment of a condition or disease which is alleviated by the activation of retinoic acid receptors (RAR). The invention also relates to pharmaceutical compounds comprising such compounds, and related methods of treatment. In an aspect, the invention relates to a method of screening compounds for therapeutic potential in the treatment of a condition or disease which is alleviated by the activation of retinoic acid receptors (RAR). Aspects of the invention relate to novel compounds of formula I in which at least one of A1 to A3 is N or at least one of A4 is CR12 or A5 is CR13 in which R12 / R13 is halogen.

The present invention relates to use of a personal care composition comprising a heteroaromatic compound selected from nicotinic acid, picolinic acid, niacin, niacinamide, picolinamide and mixtures thereof, a retinoic acid precursor selected from retinyl esters, retinol, retinal, retinoic acid and mixtures thereof, a resorcinol compound; and an oil-soluble sunscreen, for improving the barrier function of skin.