Parent selection and matching method for stevia rebaudiana bertoni leaf quality breeding

Abstract

The invention discloses a parent selection and matching method for stevia rebaudiana bertoni quality breeding, and belongs to the field of crop quality breeding. The method comprises the following steps of detecting compounds contained in the leaves of a breeding material, extracting qualitative and quantitative information, and evaluating the utilization potential of the breeding material based on the improvement direction of the content of the target compounds. The computable and more accurate parent selection and matching method for stevia rebaudiana bertoni leaf quality breeding is established. According to the accurate parent selection and matching strategy for stevia rebaudiana bertoni quality breeding, the stevia rebaudiana bertoni quality breeding efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of crop quality breeding, and in particular relates to a quality breeding parent matching method for improving the content of related compounds in stevia leaves. Background technique [0002] Stevia (also known as "Stevia", Stevia rebaudiana Bertoni) is a perennial herbaceous plant of the Asteraceae Stevia genus native to the Oman Mountains in South America. Steviol glycosides, a tetracyclic diterpenoid compound with 150-300 times of sucrose and 1 / 300 calories of sucrose, is widely planted and processed. Since its introduction in the 1970s, my country has developed into the world's most important stevia planting and processing base. [0003] With the improvement of consumption level, there are more and more people with diabetes and weight control, and the market demand for stevia glycosides from natural sources is increasing. The selection of new stevia varieties is directly related to the sustainable development of ...

AI Technical Summary

Problems solved by technology

[0008] Since stevia is a self-incompatible plant, the previous breeding of stevia was based on experience. The breeding strategy commonly used by breeders is the "youyou" hybrid combination, that is, for the target compound of breeding, select a single plant with a high content of the target compound in the leaves. To prepare a hybrid combination between strains, due to the wide variety of compounds including steviol glycosides in the leaves of Stevia rebaudiana, and the mutual transformation between the compounds, the compound information relied on by stevia breeders is limited, and only a single or a limited number of compounds are used. The content information is used for hybrid combination preparation, and the breeding efficiency is low

In order to achieve breeding goals, breeders can only prepare as many hybrid combinations as possible, which is costly and has a low success rate

[0009] Similarly, since stevia is a self-incompatible plant with complex genetic background, it is difficult to obtain recombinant inbred lines. The range of variation is large, and breeders can only test a large number of lines of the offspring of the prepared hybrid combination one by one.

However, on the one hand, due to the many types of compounds including steviol glycosides in Stevia rebaudiana leaves, the commonly used high performance liquid phase separation and detection method has high detection costs, low separation efficiency, and little information about the extracted compounds.

On the other hand, due to the large variety of compounds, the time required for the separation and detection of each sample is as long as 1.0 hours or more, and the time cost is high.

[0010] In general, the existing high-performance liquid phase detection method has low separation efficiency and little information on the obtained compounds, which makes Stevia breeders unable to effectively prepare hybrid combinations on the basis of comprehensive evaluation of various breeding materials, resulting in low breeding efficiency

At the same time, due to the complex genetic background of the hybrid parents of Stevia rebaudiana, the degree of variation of the hybrid offspring is large, the time and equipment costs for the detection of breeding materials and hybrid offspring are high, and the two major constraints on the detection combined with the empirical hybridization combination preparation method seriously restrict the development of Stevia rebaudiana. The efficient development of breeding work also restricts the sustainable development of stevia industry

The root cause is that stevia breeders lack an effective method for extracting information on compounds contained in breeding materials and their hybrid progeny leaves

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