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Torch tree root extract, preparation method thereof and application of torch tree extract in killing ostrinia nubilalis

A technology of corn borer and extraction equipment, applied in the agricultural field, can solve problems such as farmland ecosystem pollution, achieve the effect of suppressing insect pests and reducing environmental pollution

Inactive Publication Date: 2021-08-10
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a preparation method of torch tree root extract and its application in killing corn borer, so as to solve the problem of farmland ecological system pollution caused by long-term use of insecticides to kill corn borer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Add the root of torch tree into methanol with a volume ratio of 1:5 and soak for 20 minutes, then put it into an ultra-high pressure extraction equipment for extraction, the extraction time is 3 minutes, and the extraction pressure is 100MPa, filter the extracted solution, and the filter residue is used for standby, and recovered under reduced pressure Methanol, concentrated, freeze-dried to obtain alcohol-soluble substances;

[0020] Step 2: Soak the residue of step 1 in water with a volume ratio of 1:5 for 20 minutes, and put it into an ultra-high pressure extraction equipment for extraction. The extraction time is 5 minutes, the extraction pressure is 100 MPa, and the number of extractions is 2 times. The filtrates are combined, filtered and concentrated , freeze-dried to obtain water-soluble substances.

Embodiment 2

[0022] Step 1: Add the torch tree root into methanol with a volume ratio of 1:10 and soak for 15 minutes, then put it into an ultra-high pressure extraction equipment for extraction, the extraction time is 2.5 minutes, and the extraction pressure is 75MPa, filter the extracted solution, filter the residue for later use, and decompress Methanol was recovered, concentrated, and freeze-dried to obtain alcohol-soluble substances;

[0023] Step 2: Soak the residue of step 1 in water with a volume ratio of 1:10 for 15 minutes, and then put it into an ultra-high pressure extraction equipment for extraction. The extraction time is 4 minutes, the extraction pressure is 75 MPa, and the number of extractions is 2 times. The filtrates are combined and concentrated after filtration. , freeze-dried to obtain water-soluble substances.

Embodiment 3

[0025] Step 1: Add the root of torch tree into methanol with a volume ratio of 1:15 and soak for 10 minutes, then put it into an ultra-high pressure extraction equipment for extraction, the extraction time is 3 minutes, and the extraction pressure is 50MPa, filter the extracted solution, and the filter residue is used for standby, and recovered under reduced pressure Methanol, concentrated, freeze-dried to obtain alcohol-soluble substances;

[0026] Step 2: Soak the residue of step 1 in water with a volume ratio of 1:15 for 10 minutes, and put it into an ultra-high pressure extraction equipment for extraction. The extraction time is 3 minutes, the extraction pressure is 50 MPa, and the number of extractions is 2 times. The filtrates are combined, filtered and concentrated , freeze-dried to obtain water-soluble substances.

[0027] The effects of the present invention will be further described below through experimental examples.

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PUM

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Abstract

The invention relates to a torch tree root extract, a preparation method thereof and application of the torch tree root extract in killing ostrinia nubilalis, and belongs to the technical field of agriculture. The preparation method comprises the following steps: adding torch tree roots into methanol with the volume ratio of 1: (5-15), soaking for 10-20 minutes, adding into ultrahigh-pressure extraction equipment, extracting, filtering an extracted solution, reserving filter residues for later use, recovering methanol under reduced pressure, concentrating, and freeze-drying to obtain an alcohol-soluble substance; and soaking residues in water with the volume ratio of 1: (5-15) for 10-20 minutes, adding the residues into the ultrahigh-pressure extraction equipment, extracting for two times, mixing filtrates, filtering, concentrating, and freeze-drying to obtain a water-soluble substance. Active ingredients for inhibiting corn borers on corn leaves in the torch tree roots are possibly alcohol, carboxylic acid, flavonoid substances and total phenol components in the torch tree roots, and a torch tree root leaching solution is used for replacing a conventional pesticide to inhibit insect pests such as the corn borers on the corn leaves, so that environmental pollution is reduced, and normal growth of the corn is not influenced while the corn borer pests on the leaves are inhibited.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a root extract of torch tree and its preparation method and its application in killing corn borer. Background technique [0002] Allelopathy refers to the phenomenon that plants (microorganisms) release allelopathic active substances into the environment as donors, thereby directly or indirectly affecting the morphogenesis of themselves or other plants. Plant allelopathy was first proposed by German scientist Molisch in 1937, and explained it as: plants (microbes) interact through chemical substances, and this interaction has two sides (promotion or inhibition). In 1970, allelopathy was redefined by Rice, and it was first proposed that plant allelopathy is the release of allelopathic active substances into the surrounding environment by plants during their growth and development, thereby causing toxic effects on other plants. [0003] Northeast my country is a ve...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P7/04
CPCA01N65/00A01N65/08
Inventor 曲同宝赵春莉周旭丹彭玉兰杜雪罗合一李美玄
Owner JILIN AGRICULTURAL UNIV