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Construction method of persistent gouty arthritis animal disease model

A technology of gouty arthritis and construction method, applied in the directions of pharmaceutical formulations, organic active ingredients, active ingredients of heterocyclic compounds, etc., can solve the problems of difficult gene editing, high experimental cost, difficult research, etc., and achieve a good research platform, Simple and repeatable effects

Pending Publication Date: 2021-08-10
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology

Due to the low survival rate, the difficulty of gene editing, and the high cost of experiments, it is difficult to fully apply to further research
Other investigators have achieved good results by simulating gouty synovitis with intraperitoneal or subcutaneous injections of MSU, but this balloon approach is not suitable for studies of chronic gouty arthritis that focus on changes in the synovium, cartilage, and bone

Method used

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  • Construction method of persistent gouty arthritis animal disease model
  • Construction method of persistent gouty arthritis animal disease model
  • Construction method of persistent gouty arthritis animal disease model

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Embodiment 1

[0041] Such as figure 1 , the present embodiment provides a method for constructing a persistent gouty arthritis animal disease model, comprising the steps of:

[0042] Step 1, anesthetize the animal: induce anesthesia by inhaling 3% isoflurane, and maintain anesthesia by inhaling 1.5% isoflurane;

[0043] Step 2: Perform bilateral knee joint surgery: make an 8mm incision on the outside of the joint; cut the joint skin and joint capsule layer by layer along the outside of the patellar ligament to expose the knee joint cavity;

[0044] Step 3, using a Kirschner wire (diameter 1.5mm, depth 2mm) to damage the femoral intercondylar fossa cartilage;

[0045] Step 4, embedding MSU: implant 0.2ml of MSU crystal suspension (100mg / ml) into the joint cavity; clean and suture all wounds;

[0046] Step 5, repeat the above operation at an interval of 5 days, and repeat it 3-5 times, and then the animal disease model of persistent gouty arthritis can be successfully constructed.

[0047]...

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Abstract

The invention provides a construction method of a persistent gouty arthritis animal disease model. The method comprises the following steps: embedding an MSU crystal in an articular cavity for 3-5 times at an interval of 3-5 days; and before an MSU crystal is embedded in an articular cavity, damaging femoral intercondylar fossa cartilage to cause cartilage defects. According to the method, the MSU embedding mode and frequency are redesigned, the pathophysiological process of recurrent gout attack is simulated by adopting repeated intervention measures of sufficient MSU, the operation is simple and repeatable, complications such as incision-related infection and MSU leakage are avoided, a producible, economical and accurate model is provided for in-vivo research of MSU deposition and local structure erosion in gouty arthritis, and a good research platform is further provided for research of related diseases.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal disease model of persistent gouty arthritis. Background technique [0002] Gout is a systemic disease caused by uric acid disorder, and its incidence and disease burden are increasing day by day. The main pathological mechanism of gout is elevated serum uric acid concentration (over 6.8 mg / dL) and intra-articular crystal deposition of supersaturated urate (MSU) crystals deposited on the surface of cartilage, synovium or tendon. Repeated inflammatory responses gradually erode joint structures, resulting in joint pain and impaired function. [0003] Animal disease models are an important means and approach to study the diagnosis and treatment of this disease. In the past, animal models of gout were mainly divided into hyperuricemia models, local injection models and gene knockout models. Intra-articular or subcutaneous injecti...

Claims

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Application Information

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IPC IPC(8): A61K31/522A01K67/027
CPCA61K31/522A01K67/027A01K2267/035
Inventor 徐翰林陈子怡薛啸傲华英汇
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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