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Preparation process of supercritical nanoparticle for hyaluronic acid

A technology of hyaluronic acid and nanoparticles, which is applied in the direction of emulsion transportation, bulk transportation, bulk chemical production, etc., can solve the problems of complex preparation and inability to prepare nanoparticles, and achieve less wall material consumption and excellent biological Compatibility and biodegradability, high embedding efficiency

Pending Publication Date: 2021-08-10
胡勇刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent CN106890097A discloses the preparation of oil-in-water particle emulsifiers and cosmetics, self-assembled into colloidal particles through hydrophilic and hydrophobic interactions, so that it has a good emulsification effect; this method uses the traditional emulsification method to modify hyaluronic acid Emulsification, preparation is more complicated
This method only performs amphiphilic modification of hyaluronic acid, but cannot prepare nanoparticles

Method used

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  • Preparation process of supercritical nanoparticle for hyaluronic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take hyaluronic acid (HA) and add deionized water, stir to dissolve. Adjust the pH to 5.5, add a catalyst, and react for 12 hours; then add polydopamine (PDA), stir at room temperature for 24 hours, and extract the catalyst by ethanol reflux to obtain the product polydopamine-modified hyaluronic acid (PDA@HA); take 6g of PDA@HA, inject In the CO2 fluid reactor, add Tween 80 and linseed oil, cover; open the CO2 2 The steel cylinder is pressurized by a high-pressure pump and enters the fluid reactor; 100mL of purified water in the storage tank is injected into the reactor at a speed of 10mL / min. Adjust the pre-expansion pressure to 15MPa, the pre-expansion temperature to 338K, and the magnetic stirring power to 20MPa, and stir for 30 minutes until the microemulsion is fully formed.

[0047] Then set the pressure of the collection tank to be 5MPa, the temperature to be 298K, control the supercritical CO2 precipitating conditions, release the pressure quickly, and the micr...

Embodiment 2

[0049] Take hyaluronic acid (HA) and add deionized water, stir to dissolve. Adjust the pH to 5.5, add a catalyst, and react for 12 hours; then add polydopamine (PDA), stir at room temperature for 24 hours, and extract the catalyst by ethanol reflux to obtain the product polydopamine-modified hyaluronic acid (PDA@HA); take 6g of PDA@HA, inject In the CO2 fluid reactor, add Tween 80 and linseed oil, cover; open the CO2 2 The steel cylinder is pressurized by a high-pressure pump and enters the fluid reactor; 100mL of purified water in the storage tank is injected into the reactor at a rate of 10mL / min; the pre-expansion pressure is adjusted to 20MPa, the pre-expansion temperature is 338K, and the magnetic stirring power is 20MPa. The stirring time is 30min until the microemulsion is fully formed.

[0050] Then set the pressure of the collection tank to be 5MPa, the temperature to be 298K, and control the supercritical CO 2 Precipitation conditions, rapid release of pressure, th...

Embodiment 3

[0052] Add hyaluronic acid (HA) to deionized water, stir to dissolve; adjust the pH to 5.5, add catalyst, and react for 12 hours; then add polydopamine (PDA), stir at room temperature for 24 hours, and ethanol reflux to extract the catalyst to obtain the product modified polydopamine Hyaluronic acid (PDA@HA); take 6g of PDA@HA, inject it into the CO2 fluid reactor, add Tween 80 and linseed oil, seal the cap; open the CO2 2 The steel cylinder is pressurized by a high-pressure pump and enters the fluid reactor; 100mL of purified water in the storage tank is injected into the reactor at a rate of 10mL / min; the pre-expansion pressure is adjusted to 25MPa, the pre-expansion temperature is 338K, and the magnetic stirring power is 20MPa. The stirring time is 30min until the microemulsion is fully formed.

[0053] Then set the pressure of the collection tank to be 5MPa, the temperature to be 298K, control the supercritical CO2 precipitating conditions, release the pressure quickly, an...

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Abstract

The invention relates to a preparation process of supercritical nanoparticle for hyaluronic acid, The preparation process comprises the process flow including the steps of S1, preparing a hyaluronic acid grafted polydopamine product; S2, preparing a micelle solution; S3, adding a core material, a wall material and a cosurfactant into a supercritical reaction kettle; S4, when the temperature of the reaction kettle reaches the set temperature, opening a gas cylinder to introduce CO2 gas into the reaction kettle, replacing air in the kettle, and then increasing the pressure to reach the set pressure in the kettle; S5, preparing a supercritical microemulsion; and S6, rapidly spraying the supercritical microemulsion through a nozzle at a certain flow rate for separation to form grease nanoparticles, thereby obtaining the finished product. According to the invention, toxic chemical emulsifiers are avoided, and the biological activity of the nanoparticles is ensured.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to a preparation process of supercritical nanoparticles of hyaluronic acid. Background technique [0002] The emulsions prepared by solid particles instead of traditional small molecule surfactants to stabilize the oil-water interface are called Pickering emulsions, and such surface-active solid particles are called particle emulsifiers. Granular emulsifiers have the characteristics of high emulsification efficiency and low toxicity. Among them, macromolecular self-assembled colloidal particles have become one of the most promising particle emulsifiers because of their good molecular designability and structural controllability. [0003] Hyaluronic acid (HA), also known as "hyaluronic acid", is an anionic acidic mucopolysaccharide composed of two disaccharide units D-glucuronic acid and N-acetylglucosamine. It has good biocompatibility and moisturizing properti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/12C08L87/00C08L91/00C08G81/00A61K9/16A61K9/107C08L5/08
CPCC08J3/126C08G81/00A61K9/1652A61K9/1641A61K9/1664A61K9/1623A61K9/1075C08J2387/00C08J2487/00C08J2391/00C08J2491/00Y02P20/54
Inventor 胡勇刚
Owner 胡勇刚