Saccharomyces cerevisiae strain for synthesizing squalene and application thereof

A technology of Saccharomyces cerevisiae and squalene, applied in the field of genetic engineering, can solve the problems of limited output and long fermentation cycle, and achieve the effect of strengthening metabolic flow
CN113234610AActive Publication Date: 2021-08-10JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-08-10

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Abstract

The invention discloses a saccharomyces cerevisiae strain for synthesizing squalene and an application of the saccharomyces cerevisiae strain, and belongs to the technical field of genetic engineering. According to the invention, tHMG1 and IDI1 are integrated at a multi-copy site Ty2 of saccharomyces cerevisiae, so that synthesis and accumulation of squalene are remarkably promoted, NADH-dependent HMG1 is heterologously expressed, the redox level in yeast cells is balanced, a promoter HXT1 with a remarkable inhibition effect on ERG1 transcription is further replaced, conversion of squalene to the downstream is hindered, ACL1 and ACL2 are heterologously expressed, PEX11 and FOX3 are over-expressed, accumulation of a squalene synthesis precursor acetyl coenzyme A in cytoplasm is enhanced through beta oxidation, accumulation of squalene is further promoted, and the squalene yield can reach 1253.4 mg / L after saccharomyces cerevisiae is fermented for 96 h.
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Description

technical field

[0001] The invention relates to a Saccharomyces cerevisiae strain for synthesizing squalene and an application thereof, belonging to the technical field of genetic engineering. Background technique

[0002] Squalene (squalene) is an important terpenoid compound, which is the precursor of many bioactive compounds, such as steroids and hopene. At the same time, squalene has many values, such as being used in the cosmetic industry as a moisturizing ingredient, due to His antioxidant properties can be used as a moisturizer, and squalene is also beneficial to human health, including anti-tumor, anti-fungal / fungal, enhancing the body's immunity, and lowering cholesterol, such as anti-inflammatory, anti-aging, anti-oxidation, etc. Squalene is also widely used to make stable emulsions, such as vaccines, drugs and other medicinal substrates. The traditional method extracts from shark liver by illegal means or inefficiently extracts from plants, but in view of its lim...

Claims

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