Detection kit
A detection kit, mannitol technology, applied in measurement devices, instruments, biological material analysis, etc., can solve problems such as autoimmune nephropathy detection, achieve convenient storage and transportation conditions, reduce costs, and save antigens.
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Problems solved by technology
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Examples
Embodiment 1～3
[0095] 1) The chip array design and the specific distribution of antigens and reference points are shown in Table 1 and Table 2:
[0096] Table 1
[0097] · · · · · · · · · · · · · · · · · · · ·
[0098] Table 2
[0099] PC NC GBM LAMP-2 Endothelial cells SC S1 PR3 PLA2R nucleosome EC S2 MPO THSD7A dsDNA Loc S3 LF PT protein C1q
[0100] 2) The specific coating process:
[0101] First, dilute antibodies and associated proteins as follows:
[0102] The PC, NC, S1, S2, S3, and EC points in the array are coated with human IgG at 2 μg / ml, 0.01 μg / ml, 0.5 μg / ml, 2 μg / ml, 4 μg / ml, and 2 μg / ml, respectively, diluted The buffer is CB buffer at pH 9.6 (containing 2.5% PEG4000, 5% trehalose, 0.05% Proclin300, and 15% glycerol).
[0103] SC dots were coated with 2 μg / ml goat anti-human IgG antibody, and the dilution buffer was CB buffer at pH 9.6 (same as abov...
Embodiment 4
[0126] Embodiment 4 accuracy evaluation
[0127] 1. 10 cases of anti-GBM IgG positive sera and 10 cases of anti-GBM IgG negative sera screened by Abnova company ELISA kit were tested with the chip kit prepared in Examples 1 to 3, and the results were shown in Table 5 below (+ indicates positive, - means negative).
[0128] The chip test anti-GBM IgG serum result that table 5 embodiment 1~3 prepares
[0129]
[0130] 2. 10 cases of anti-PR3 IgG positive sera and 10 cases of anti-PR3 IgG negative sera screened by EUROIMMUN company ELISA kit were tested with the chips prepared in Examples 1 to 3, and the results were shown in Table 6 below (+ indicates positive, - indicates negative ).
[0131] The anti-torch-IgG antibody spectrum chip test anti-PR3 IgG serum result that table 6 embodiment 1~3 prepares
[0132]
[0133] 3. 10 cases of anti-MPO IgG positive sera and 10 cases of anti-MPO IgG negative sera screened with the Invitrogen company ELISA kit were tested with the ...
Embodiment 5
[0164] Embodiment 5 sensitivity and specificity test result
[0165] Patient samples were 85 positive serum samples from patients with autoimmune nephropathy diagnosed through renal puncture and clinical manifestations, and 102 negative serum samples from normal healthy people.
[0166] Table 17
[0167]
[0168] As can be seen from the above data, the chip kit of the present invention can reach 98.8% in sensitivity (positive coincidence rate) to clinical autoimmune nephropathy patients (autoimmune nephropathy patients diagnosed jointly by renal puncture and clinical symptoms), and the specificity (Negative correct rate) reaches 100%, indicating that the kit of the present invention has high sensitivity and good specificity, and can provide more accurate and comprehensive reference and guidance for the diagnosis of autoimmune nephropathy.
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Abstract
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