Medicine and method for preventing and treating citrus liberobacter asiaticum
A technology for citrus and medicine, applied in the agricultural field, can solve the problems of limited effect, no effective control method for citrus Huanglongbing, unproven effect continuity, etc., and achieves the effect of remarkable effect, remarkable treatment and long lasting effect.
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[0049] 1. Experimental materials and methods
[0050] 1.1. Experimental plant material
[0051] (1) Cultivate susceptible branches in the laboratory: In April 2016, we grafted Shatangju trees infected with Huanglongbing onto healthy 2-year-old Shatangju seedlings, and then kept them in the culture room for cultivation. After 10 months, the typical symptoms of Huanglongbing appeared on the leaves with blocked veins and spots.
[0052] (2) Field test field I: from orchards in Guangdong Province, China - about 10-year-old Shatang orange trees infected by Huanglongbing.
[0053] (3) Field test field II: about 10-year-old navel orange trees infected with Huanglongbing disease from an orchard in Ganzhou City, Jiangxi Province, China.
[0054] (4) In order to minimize the influence of uneven distribution of Huanglongbing bacteria, the sampling method is as follows:
[0055] In the experiment of soaking the isolated branches with copper ammonia, sampling method: collect one leaf at...
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