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New application of manganese chloride or composition containing manganese chloride in preventing or treating African swine fever

A technology of African swine fever and manganese chloride, which is applied to medical preparations containing active ingredients, organic active ingredients, inorganic active ingredients, etc. Effects of suppressing expression, suppressing gene copy number and virus titer

Active Publication Date: 2021-08-20
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wherein document (Melchjorsen J, et al.Early Innate Recognition of Herpes Simplex Virus in Human Primary Macrophages Is Mediated via the MDA5 / MAVS-Dependent and MDA5 / MAVS / RNA Polymerase III-Independent Pathways.) discloses RNA polymerase III inhibitor ML60218 There is no inhibition on the levels of interferon IFN-β and TNF-α after HSV-1 infection, that is, ML-60218 cannot inhibit the replication of HSV-1 virus

Method used

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  • New application of manganese chloride or composition containing manganese chloride in preventing or treating African swine fever
  • New application of manganese chloride or composition containing manganese chloride in preventing or treating African swine fever
  • New application of manganese chloride or composition containing manganese chloride in preventing or treating African swine fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 ml-60218 and MNCL 2 Effects of infection of African swine fever virus infection and gene transcriptional expression

[0034] Gene copy number

[0035] Culture pig lung macrophages (PAM, 1 × 10) with RPMI 1640 + 10% FBS medium in 12-well plates 6 / Hole), the experimental groups use ML-60218 (50μm), MNCL, respectively. 2 (0.2μm) and ML-60218 (50μm) + MNCL 2 (0.2 μm) After 2 h, an ASFV CN / GS / 2018 strain (0.1 moi) 24 h was infected; the control group was treated with DMSO (1%) for 2 h, and the ASFV CN / GS / 2018 strain (0.1 moi) 24h was infected. Cells were collected, and the cell culture was collected repeatedly after -80 ° C, centrifugally, centrifuged, and take it. Use Q-PCR method to detect ML-60218 + MNCL 2 The impact on ASFV virus replication is used to calculate the amount of ASFV viral gene copies using the formula.

[0036] The total volume of the Q-PCR reaction system was 25 μL, including 2 × pro TAQ Hs Probe premix12.5 μL, 1 μl of the upper and lower ...

Embodiment 2

[0046] Example embodiments 2ML-60218 and MnCl 2 Cytotoxic composition

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PUM

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Abstract

The invention belongs to the technical field of African swine fever treatment, and particularly relates to new application of manganese chloride (MnCl2) or a composition containing manganese chloride in preventing or treating African swine fever. The invention firstly finds that manganese chloride can inhibit the expression of African swine fever virus protein p72, inhibit the gene copy number and virus titer of African swine fever virus, can inhibit the replication and infection of ASFV, and is used for preventing or treating African swine fever; meanwhile, the invention finds that the composition of manganese chloride and ML-60218 can significantly inhibit the expression of African swine fever virus protein p72 and inhibit the replication and infection of ASFV; and the composition has an ASFV inhibition effect obviously better than that of single use of manganese chloride or ML-60218, and can be used for preventing or treating African swine fever.

Description

Technical field [0001] The present invention belongs to the technical field of African swine fever, and more particularly to a combination of chloride or a composition containing chloride for preventing or treating African swine fever. Background technique [0002] African Swine Fever (ASF) is an acute vigorous infectious disease characterized by the bleeding of pig fever and pigs in African swine fever virus (ASFV) infection. It is as high as 100% of the mortality rate of pigs. . Due to the still no effective vaccine and special effects of drugs, the African swine fever will only be controlled by a cambrid, but this means not only lead to economic losses, but can not meet the needs of my country's large-scale pigs. Therefore, how to effectively control the ASF epidemic is one of the huge challenges faced by the world pig industry, and it is also a major strategic issue in my country ASF prevention and control. [0003] P30 and P72 are key structural proteins in ASFV, and can inh...

Claims

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Application Information

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IPC IPC(8): A61K33/32A61P31/20A61K31/4155
CPCA61K33/32A61K31/4155A61P31/20A61K2300/00
Inventor 郑海学李丹易佳敏彭江玲杨文萍张敬茹毅田宏杨帆刘永杰卢炳洲张克山
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI