Methods for making high intensity sweeteners
A technology of sequence and recombination of host cells, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve the problems of delayed onset and residue of sweetness
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Embodiment 1
[0314] Example 1: Production of simonoside I
[0315]
[0316] As disclosed herein, simonoside I may be an intermediate mogroside compound for the production of Compound 1 disclosed herein. For example, symanoside I can be hydrolyzed to produce mogroside IIIE, which can then be used to prepare compound 1. For example, the method for preparing simonoside I may comprise: combining mogroside with expression of one or more UDP glycosyltransferases, CGTases, glycosyltransferases, glucansucrase, cellulase, β-glucosylase Recombinant host cell contacts of glycosidases, amylases, transglucosidases, pectinases and glucanases. For example, recombinant cells expressing pectinase from Aspergillus aculeatus can be used.
[0317]As another non-limiting example, the method for producing simonoside I may include: making mogroside V, mogroside IV E , Mogroside V, Mogroside III E , 11-deoxy-mogroside V, 11-oxo-mogroside V, mogroside VI, mogroside IV A , Mogroside II A , Mogroside II ...
Embodiment 2
[0318] Example 2: Mogroside IV E production
[0319]
[0320] As disclosed herein, mogroside IV E may be an intermediate mogroside compound for the production of Compound 1 disclosed herein. For example, mogroside IV from mogroside V can then be used E to produce compound 1. For example, for the production of mogroside IV E The method may comprise: making mogroside V express one or more UDP glycosyltransferases, CGTases, glycosyltransferases, dextran sucrase, cellulase, β-glucosidase, amylase, transglucose Recombinant host cell contacts of glycosidases, pectinases and dextranases. As another example, a recombinant cell can contain a gene encoding a pectinase. Pectinase may be encoded by a gene from Aspergillus aculeatus.
[0321] As another example, for the production of mogroside IV E The method may include: making mogroside V, simonoside I, isomogroside V, mogroside III E , 11-deoxymogroside V, 11-oxomogroside V, mogroside VI, mogroside IV A , Mogroside II A ...
Embodiment 3
[0322] Example 3: Mogroside III E production
[0323]
[0324] As disclosed herein, mogroside III E may be an intermediate mogroside compound for the production of Compound 1 disclosed herein. For example, Mogroside II can be A Glycosylation to produce mogroside IIIE, which was then used to produce compound 1.
[0325] As another example, for the production of mogroside III E The method may include: making mogroside V, mogroside II A , Symonoside I, Mogroside IV E , Mogroside V, 11-deoxymogroside V, 11-oxomogroside V, mogroside VI, mogroside IV A , Mogroside II A , Mogroside II A1 , Mogroside II A2 , Mogroside I A , 11-oxomogroside VI, 11-oxomogroside III E , 11-oxomogroside IV E , Mogroside I E , Mogrosin, 11-oxomogrosin, mogroside II E , Mogroside III A2 and one or more of mogroside III are contacted with a recombinant host cell expressing UDP glycosyltransferases, CGTases, glycosyltransferases, glucansucrase, cellulase, β-glucosyltransferase, One or mor...
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