Microfluidic device and manufacturing method thereof, and microbead screening method

Abstract

The invention provides a microfluidic device and a manufacturing method thereof, and a microbead screening method. The microfluidic device comprises a first substrate and a second substrate which are arranged in a stacked mode, wherein the first substrate comprises a first input flow channel, a first flow channel and a first output flow channel which sequentially communicate with each other; the second substrate comprises a second input flow channel, a second flow channel and a second output flow channel which communicate with each other in sequence; and the first flow channel and the second flow channel comprise a first overlapping part, the first overlapping part communicates with the first flow channel and the second flow channel, and the projection of the first overlapping part on the plane where the first substrate is located is located in the projection of the first flow channel on the plane where the first substrate is located, and is also located in the projection of the second runner in the plane where the first substrate is located. The microfluidic device is formed by etching and bonding, and is simple in structure and easy to manufacture. When microbead samples with different densities enter the first flow channel, a part of microbeads float or sink, enter the second flow channel through the first overlapping part and are extracted, so a microbead screening and extracting process is simple and easy to operate, and the risk of microbead pollution can be reduced.

Description

technical field [0001] The present application relates to the field of microfluidic technology, in particular, to a microfluidic device, a manufacturing method thereof, and a microbead screening method. Background technique [0002] Microfluidics refers to a system that uses micro-pipes with a size of tens to hundreds of microns to process or manipulate tiny fluids with volumes ranging from nanoliters to attoliters, involving emerging interdisciplinary subjects such as chemistry, fluid physics, microelectronics and biology. In the fields of biology and chemistry, it is usually necessary to screen and extract target microbeads from different types of microbeads, so that the subsequent target microbeads can perform chemical reactions and detection. The existing microbead screening methods such as displacement reaction, and then use centrifugation, precipitation and other steps to extract according to the situation, will easily lead to damage and rupture of the microbeads, whic...

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Problems solved by technology

[0003] In view of this, the present invention provides a microfluidic device, a manufacturing method thereof, and a microbead screening method to solve the problems of cumbersome steps and high risk of microbead contamination in the prior art when screening and extracting microbeads

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