Centrifugal microfluidic detection device and centrifugal microfluidic detection system

[0058] Next, the technical solutions in the embodiments of the present invention will be apparent from the embodiment of the present invention, and it is clearly described, and it is understood that the described embodiments are merely embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, there are all other embodiments obtained without making creative labor without making creative labor premises.

[0059] like Picture 1-1 1, the centrifugal microfluidic detection apparatus according to this invention comprises: a power supply module 100, control module 200, rotation module 300, lysis module 400, a heating module 500, image detection module 600, visual color detection module 700, and chip tray 800.

[0060] Detecting the centrifugal microfluidic device, a chip tray 800 for carrying the chip 900, chip 900 is a centrifugal micro-fluidic chip and a test sample, a driving chip module 300 rotates the rotary tray 800, the sample can be lysed module 400 of the chip 900 sample lysis in the chamber 902, a heating module 500 to heat the chip 900 of the reaction chamber 907, imaging detector module 600 can be on-chip test strip cavity 900 is an imaging detector 908, visual color detection module 700 to the reaction chamber 907 to the chip 900 absorbance detection and color detection, control module 200 for controlling the rotation of the module 300, lysis module 400, a heating module 500, imaging module 600 is detected, and the visual color detection module 700 work, the power supply module 100 to control module 200, the rotation module 300, lysis module 400, a heating module 500, imaging module 600 is detected, and the visual color detection module 700 power.

[0061] It will be appreciated that the above visual color detection module 700 without performing the color detection absorbance detection, color detection is not performed when said detected visual color absorbance detection module 700 is performed. The test strip chamber 908 is preset with the test strip.

[0062] The control module 200 the rotation block 300, lysis module 400, a heating module 500, imaging module 600 is detected, and the visual color detection module specific control mode 700 is set according to actual needs, as long as the detection can be completed, the present embodiment which is not limited.

[0063] Incidentally, the centrifugal microfluidic detection apparatus using both the reaction chamber 907 is provided with a test strip without chip 900 cavity 908 can also be used provided with a strip cavity 908 and no reaction chamber 907 of the chip 900, You can also use a test strip is provided with a cavity 908 and chip 900 of the reaction chamber 907.

[0064] Centrifugal microfluidic detection apparatus according to an embodiment of the present invention, by providing the imaging detection module 600, an imaging detector can be realized; 700, color detection can be achieved only through visual color detection module, absorbance detection can be achieved. Thus, the centrifugal microfluidic device can simultaneously implement two detecting optical detector, an optical detector can be achieved only compared with the prior art, effectively improving the universality; Further, the image detection module 600 can also be used in immunoassays It can be used for nucleic acid detection, the above-described visual color detection module 700 can also be used in immunoassays for the detection of nucleic acid, the detection of the centrifugal microfluidic device can achieve both immunoassays and nucleic acid detection, improve the detection performance.

[0065] To avoid contamination and interference in the detection process outside the centrifugal microfluidic device further includes a detector housing, such as Figure 2-4 As shown in the control module 200, module 300 is rotated, the cleavage block 400, a heating module 500, imaging module 600 is detected, the visual color detection module 700 and chip trays 800 are located within the housing, the power supply module 100 is located outside the housing.

[0066] Optionally, the outer housing 20 is provided with a display screen, power adapter interface 30, communication interface 40, and a switch 50, such Figure 4 Indicated.

[0067] The control module 200, rotation module 300, lysis module 400, a heating module 500, image detection module 600, visual color detection module 700 and chip trays 800 are located within the housing, the control module 200, rotation module 300, lysis module 400, a heating module 500, the detection module 600 and the imaging visual color detection module 700 are electrically connected by the power adapter 30 and the interface supply module 100, a communication interface 40 through the communication control module 200 is connected with external equipment, a switch 50 for controlling the electrical connector start and stop off and a control module 200, the display 20 is connected to the communication control module 200 and for displaying the detection result.

[0068] Specifically, the communication interface 40 may be connected through the communication transmission line and external devices for data transmission. The above-described external apparatus may be a PC terminal, according to the actual need to select the type of external device. Said power supply adapter interface 30 is electrically connected by the power supply module 100 and the transmission line. 20 shows the result of the above-described image display module 600 and a detection result of said visual color detection module 700. In actual application, the display panel 20 can be selected directly 600, visual color detection module 700 connected to the communication module imaging detector.

[0069] Detecting the centrifugal microfluidic devices, by providing a housing, the other modules except the power supply module 100 are disposed within the housing, to meet the convenience of a portable, real-time inspection requirements; Further, the control module 200 is connected with the external communication interface 40 through the communication equipment, facilitate access to the detection result.

[0070] For 20, the power adapter interface 30, communication interface 40, a switch 50, and the type of the display panel, according to actual needs, the present embodiment is not limited to this embodiment.

[0071] To facilitate pick chip 900, such as Figure 3-5 Shown, the housing comprising: a base 80, connected to the base 80 and cover 10 can be opened and closed; wherein the control module 200, rotation of the tray 800 and the chip module 300 are disposed on the base 80, and the imaging module 400 cleavage detection module 600 are provided on the upper cover 10, a heating module 500 disposed on the base 80 and cover 10, visual color detection module 700 disposed on the base 80 and cover 10.

[0072] In order to facilitate opening and closing of the upper cover 10, a side of the upper cover 10 above the base 80 of the hinge. For the specific configuration of the hinge, is selected according to actual needs. Specifically, such as Figure 5 , The upper cover 10 is connected via a tight circular hole with the pin cards 70, the base 80 is provided with a receiving groove accommodating the pin 70, the pin 70 is rotated with the receiving groove, the pin 80 is fixed to the base 70 by a fixing member 60 is connected pressure is provided on the base 80, a hinge cover 10 is achieved. Specifically, in order to facilitate disassembly and maintenance, the fixing member 60 is fixed by screwing to the base member 80.

[0073] In actual application, also choose other configurations to achieve the above hinge, hingedly e.g., not limited to the embodiments; of course, choose a different manner to open and close the upper cover 10, for example, the upper cover 10 and the base 80 may removably snap, not limited to the articulated manner.

[0074] Detecting the centrifugal microfluidic device, in order to facilitate rotation of the connector module 300 and chip tray 800, such as Figure 6A Shown, the rotating module 300 includes: a drive member 310 is rotated, the output shaft member 310 fixedly connected with the rotary drive shaft and connected by magnetic and chip trays fixedly connected 800320. At this time, the control module 200 controls the rotation drive member 310 working.

[0075] Specifically, such as Figure 6B , Figure 6C with Figure 13D , One end of the connecting shaft 320 is provided with an end of the first groove 322, a chip tray 800 is provided with a second recess 802, first recess 322 and second recess 802 is provided with one magnet, another are provided with a magnet is capable of being adsorbed by adsorbent. To improve reliability, the magnet and the adsorbent are permanent magnets.

[0076]In order to ensure uniform stress and improve the attractiveness of the first recess 322 and at least two evenly distributed along the circumference of the connecting shaft 320, a first groove 802 and second groove 322 correspond. It will be appreciated that the same profile and the distribution of the second recess 802 of the first recess 322. In particular, the first recess 322 and second recess 802 are circular grooves, and are four. In practical applications, the first groove 322 may also be adjusted and the shape and number of the second recess 802, the present embodiment is not limited to this embodiment.

[0077] For ease of stationary magnets and the adsorbent, the magnet is bonded to the first recess 322, the adhesive attachment is drawn in the second recess 802; alternatively, the magnet is bonded to the second recess 802, is adsorbed an adhesive member in the first recess 322. Of course, other means can also choose fixed, for example snap the like, not limited to the above defined.

[0078] The above-described configuration, the rotation module and a simplified connection structure 300 of the chip tray 800, the rotation module 300 also reduces the chip tray 800 and the space occupied by the connector, improved compactness.

[0079] To improve reliability, the centrifugal microfluidic detection apparatus further comprising a positioning assembly, the locating assembly is positioned and connected to the shaft 320 within the plane of the chip tray 800 chip tray 800 is located.

[0080] Specifically, such as Figure 6B , Figure 6C with Figure 13D Shown, the positioning assembly comprising: a first positioning groove 803, the second positioning groove 804, the first positioning post 321 and a second positioning post 324; connecting shaft 320 and a chip tray 800, one provided with a first positioning groove 803 and second positioning grooves 804, and the other is provided with a first positioning pin 321 and a second positioning post 324; 803 accommodating the first positioning groove 321 and the first positioning pin 803 in the first positioning groove 800 of the chip tray radial stop first positioning pin 321, the second circumferential groove 804 positioned to receive the second positioning post 324 and the second positioning groove 804 in the chip tray 800 to the second limiting post 324 is positioned.

[0081] In order to increase the strength of the connecting shaft 320, the first positioning groove 803, the second positioning groove 804 disposed in the chip tray 800, a first positioning post 321 and a second positioning post 324 is provided on the connecting shaft 320. At this time, the first positioning groove 803, the second positioning groove 804 and second groove 802 may be distributed in the same circumferential direction, i.e., the first positioning groove 803, the second positioning groove 804 and second groove 802 located in the same ring on, and the first positioning groove 803, second groove 804 is positioned along the circumference of the chip tray 800 uniformly distributed. Of course, choose other distributed manner is not limited as defined above.

[0082] For ease of installation, the first positioning groove 803 and the second positioning grooves 802 are deep grooves, such as groove 803 of the first positioning and second positioning grooves 804 showed kidney-shaped, the major axis of the first positioning groove 803 and the second long axis perpendicular to the positioning groove 804.

[0083] In the practical application process, but also select the first positioning groove 803 and second groove 804 is positioned other shapes, such as rectangular groove or a circular groove, etc., and limited to the above embodiments.

[0084] The first positioning groove 803 may be one, or may be two or more, the second positioning groove 804 may be one, or may be two or more. In the practical application process can be selected according to actual needs. To simplify the structure, alternatively the first positioning groove 803 and second groove 804 are a positioning.

[0085] The connecting shaft 320 to the rotation drive member 310 of the output shaft is fixedly connected, for convenience of connection, the connecting shaft 320 and the output shaft by a threaded connection member is fixedly connected, in particular, the periphery of the connecting shaft 320 is provided for the screw to the side connecting member 325 inserted in the screw hole. The connecting shaft 320 is provided with a central mounting hole 323, the output shaft 310 of the mounting hole 323 of the rotary drive receiving member.

[0086] For the type of the rotation drive member 310, according to the actual needs, for example, the rotation member 310 to rotate the drive motor or rotating cylinder, etc., the present embodiment is not limited to this embodiment.

[0087] Detecting the centrifugal microfluidic devices, specific configuration of the heating module 500, according to actual needs. Specifically, such as Figure 7 Shown, the heating module 500 comprises a heating element, the heating element comprising a heating film 502, 502 disposed on a heating plate heating film, film disposed on a heating plate 502 and the heating temperature for detecting a temperature sensor 501. It will be appreciated that the above-described control module 200 communicates with the connected temperature sensor 501, and control module 200 for controlling the heating the heating film 502 according to the detection value of the temperature sensor 501.

[0088] The heating assembly may be one, or may be two or more. To increase the heating efficiency of the heating assembly and two are on the top side of the chip tray 800 and the bottom side. It will be appreciated that the two heating assembly is provided for the top side and the bottom side of the chip 900. Thus, to achieve a double-sided heating, the reaction chamber may be better located in the middle 900 of the chip 907 is heated to enhance the rate of temperature rise and thermal uniformity, thereby improving the heating efficiency.

[0089] The heating assembly is two, two of the heating plate, respectively, the upper heating plate 503 and the lower heating plate 504; a heating plate 503 provided on the chip 900 to the top plate 504 for heating provided on the chip is located the bottom portion 900; heating the film 502 and temperature sensor 501 are two.

[0090] When the centrifugal microfluidic detecting means comprises an upper cover 10 and the base 80, the upper heating plate 503 is fixed to the upper cover 10, the lower heating plate 504 is fixed to the base 80. Specifically, the upper heating plate 503 is adhered to the upper cover 10, the lower heating plate 504 by a threaded connection member 80 fixed to the base.

[0091] For the type of the heating film 502, according to the actual needs, for example, a heating film 502 as a PI heating the film, i.e., a polyimide film is heated.

[0092] For the type of the temperature sensor 501, also according to the actual needs, for example, a temperature sensor 501 as described above PT100 temperature sensor.

[0093] The heating plate may be a metal heating plate, may also be capable of other thermally conductive plate, as long as good thermal conductivity can be, for example, the heating plate is aluminum alloy sheet, which is not limited to this embodiment.

[0094] The heating assembly, for ease of assembly, the heating plate is bonded to the film 502 is heated, the temperature sensor 501 secured to the film 502 by heating the thermal grease. Further, the temperature sensor 501 is bonded to the heating plate.

[0095] The heating temperature control range module 500 according to actual needs, for example, controlling the heating temperature range is 10 ℃ module 500 to 100 deg.] C, the present embodiment is not limited to this embodiment.

[0096] Detecting the centrifugal microfluidic devices, for a particular type of structure and cleavage module 400, according to actual needs. Specifically, such as Figure 8 with Figure 9 As shown in the sample chamber 900 of the chip 902 is preset milling beads 912 and a stir bar 913; and the lysis module 400 includes a magnet 403, and the cleavage drive member 401 to rotate the driving magnet 403 is capable of adsorbing stirring rod 913. To improve reliability, the magnet 403 is a permanent magnet.

[0097] In the above configuration, the drive member 401 driven by cleavage of the rotating magnet 403, the magnet 403 drives the disturbance vigorously stirring bar 913, so that the sample in the sample chamber 902 is sufficiently mixed with the polishing with the grinding beads 912, so as to realize the purpose of cleavage of a sample.

[0098] It will be appreciated that the stirring rod 913 can be adsorbed magnet 403, the magnet 403 is not adsorbed milling beads 912. Specifically, the polishing beads are glass milling beads 912, 913 is a metal stirring rod stir bar. Of course, alternatively the polishing beads 912 and a stir bar 913 for other materials, as long as complete cleavage to the present embodiment is not limited to this embodiment.

[0099] In order to facilitate fixed magnet 403, the magnet 403 through a magnet tray 404 is fixed to the output shaft 401 of the drive cleavage member. Specifically, the magnet 404 is fixed to an output shaft of the tray cleavage drive member 401, a magnet 403 snap-locked to the tray 404 or the magnet 403 bonded magnets the magnet tray 404.

[0100] The magnet 403 may be one, or may be two or more, selected according to actual requirements. The shape of the magnet 403, is also selected according to actual needs, for example, the magnet 403 is square. This embodiment 403 of the magnet shape and number is not limited.

[0101] In practical applications, the sample chamber of the chip select 900 is positioned 902 for setting the bottom side 400 of the cleavage block. In particular, if the centrifugal microfluidic detection apparatus includes a base 80 and cover 10, the above-described cleavage block 400 is fixed to the upper cover 10. For ease of installation, the above-described cleavage drive member 401 is fixed to the upper cover 10. In order to facilitate fixation, the above-described selectable cleavage driven by a driving member 401 fixed to the upper cover 402 of the housing 10, specifically, the drive housing 402 by a threaded connection member 10 is fixed to the upper cover, cleavage drive member 401 is fixedly connected to the drive housing 402 .

[0102] For the type of drive member 401 in the above-described cleavage, according to the actual needs, for example, the above-described cleavage driving member 401 is a DC motor or a cylinder, which is not limited to this embodiment.

[0103] The centrifugal microfluidic detection apparatus for detecting the type of the imaging module 600, selected according to actual needs. Specifically, such as Figure 10 As shown in the image detection module 600 includes a first light source 601 and the imaging element, the imaging element 601 provided at the top of the chip 900 is used, the first light source is disposed at a side of the chip 900 and the chip 900 near the cavity of the test strip 908.

[0104] In the above-described image detection module 600, the imaging element 601 and the communication control module 200 is connected.

[0105] For the above-described type of the imaging element 601, according to actual needs, for example, the imaging element 601 is a CCD image sensor, i.e. a charge coupled device, CCD English full Charge-coupled Device. The present embodiment is not limited to this embodiment.

[0106] For the first type of light source, according to actual needs. To facilitate detection, select the first light source is an LED light source. In particular, LED light source comprises an LED lamp with 602, LED lights 602 is provided with at least two LED lights 603. Thus, it is possible from at least two directions of irradiation of the surface of the chip 900, a shadow can be avoided, so that more uniform illumination.

[0107] In order to improve the reliability of the detection result of the LED lamp 603 for emitting white light.

[0108] When the imaging detector, the rotation block 300 to be rotated to the detected chip 900 directly below the imaging element 601, this time, LED lights for illumination of the surface 603 of chip 900 from at least two slightly different directions chip 900 detecting reflected light through the rear surface 900 of the chip 601 enters the imaging element.

[0109] In practical applications, the rotating module 300 of the chip 900 to be detected by all the low speed mode are sequentially rotated by the imaging detection module 600, the completion of the final test strip forming a cavity on all the chips 900 908.

[0110] Further, the LED lamp 603 is three. Of course, also choose four of the LED lamp 603 of the above, the present embodiment is not limited to this embodiment.

[0111] When the centrifugal microfluidic detection means comprises an upper cover 80 to the base 10 and the imaging element 601 is fixed to the top of the upper cover 10, specifically, the imaging element 601 by a threaded connecting member is fixedly connected with the upper cover 10, for easy removal; first a light source fixed to a side of the upper cover 10, in order to facilitate disassembly, the first light source cover 10 attached to the card.

[0112] Detecting the centrifugal microfluidic device, the configuration of the visual color detection module 700, according to the actual needs. In particular, the visualization module 700 includes a color detection sensor and a second visual light color, visual color sensor chip is provided for the tip 900, a second light source disposed at the bottom end 900 of the chip.

[0113] For the configuration of the color visual sensor is selected according to actual needs. Specifically, such as Figure 11A with Figure 11B , The visual color sensor comprises a visual color sensor body 701, polyethylene reticle 702 and the connection line 703, visual color sensor body 701 through 200 correspond connecting line 703 to the control module, polyethylene reticle 702 disposed in the photosensitive color sensor body 701 element.

[0114] Incidentally, the color sensor body working distance of the photosensitive member 701 is required, for example, the above-mentioned working distance of 10 mm, is set according to actual needs. Working distance is the above-described photosensitive element to a distance of 900 chips.

[0115] The rotating module 300 to chip 900 is rotated to be detected visualized directly below the color sensor 701, the light detecting portion through the photosensitive poly-visual reticle 702 incoming color sensor 701. The reaction chamber 907. By rotation of the drive module 300, such that the reaction chamber 900 of the chip 907 is in a low speed mode visualized color detection sensor 701 scans. This visualization color detecting device having small and simple manner, low power consumption characteristics, using conditions suitable for real-time detection, monitoring time may be real-time or end point of a liquid color detection or absorbance detection.

[0116] For the second type of light, selected according to actual needs. To facilitate detection, the second light source is an LED light source. Specifically, such as Figure 11C As shown in the LED light source comprises: LED bracket 704, the bracket 704 is fixed to the LED of the LED lamp 707; wherein, LED holder 704 is provided with a light transmitting hole 705 for passing the light emitted from the LED lamp 707.

[0117] The light-transmissive aperture 705 may be circular holes, elliptical holes, etc., according to actual needs.

[0118] When the centrifugal microfluidic detection apparatus includes a base 80 and cover 10, the above-described LED holder 80 fixed to the base 704, the color visual sensor 10 fixed to the top of the upper cover.

[0119] For easy disassembly, of the LED holder 704 is detachably fixed to the base 80, for example of the LED holder 704 is fixed by screwing to the base member 80, or of the LED holder 704 attached to the card base 80. In practical applications, the above-described selectable LED holder 704 by screwing the base member 80 is fixed to the LED holder is provided with a through hole for the screw connecting member 706 through.

[0120] Accordingly, the above visual color sensor 10 is detachably secured to the upper cover, for example, the color sensor visualized by a threaded connection 10 is fixed to the upper cover.

[0121] The second light source, for ease of assembling the LED lamp holder 707 is bonded to the LED 704.

[0122] Based on the centrifugal microfluidic detecting means provided in the above-described embodiment, the present embodiment further provides a centrifugal microfluidic detection system, the detection of the centrifugal microfluidic system comprising: a centrifugal microfluidic detection apparatus according to the embodiment described above, and the chip 900 can be applied to the centrifugal microfluidic detecting means; wherein the chip comprises a reaction chamber 907 or 900 strip cavity 908; Alternatively, chip 900 includes a reaction chamber 907 and chamber 908 test strip, the test strip cavity 908 located downstream of the reaction chamber 907.

[0123] Due to the centrifugal microfluidic detection means to the above embodiment has the above technical effect, the centrifugal microfluidic detection system comprising the centrifugal microfluidic detection device, the centrifugal microfluidic detection system also has corresponding technical effects, this article does not then repeat.

[0124] Detecting the centrifugal microfluidic system, for the specific configuration of the chip 900, designed according to actual needs. Specifically, such as Figure 12 , The chip 900 comprises a reaction chamber 907 and chamber 908 test strip, the test strip 908 is located downstream of the chamber 907 of the reaction chamber; alternatively the die 900 further comprises: a reagent chamber 903, mixing chamber 906, the injection holes 901, located the injection hole 901 of the distal end and the sample chamber and the injection hole 901 communicates 902, located in sample chamber distal 902 ends and capable buffer chamber communicating with the sample chamber 902 904, located in the buffer chamber 904 of the distal end and with a first distribution chamber 905a communicating buffer chamber 904, reagent chambers located in the distal end 903 and a second distribution chamber capable of communicating with the reagent chamber 903 905b; wherein a first distribution chamber a second distribution chamber 905a and 905b are located in the mixing chamber the proximal end 906 and is capable of simultaneously communicating with the mixing chamber 906; the reaction chamber 907 of the mixing chamber 906 and the distal end 906 can be in communication with the mixing chamber, the test strip is located in the chamber 908 of the mixing chamber distal end 906 and can be mixed with chamber 906 communicates.

[0125] Specifically, in order to facilitate communication between the various chambers, the chip 900 is provided with a siphon passage 909 and the vent channel 910, transfer of liquid between the chamber for controlling. Specifically, the first distribution chamber 905a through the first siphon passage 906 in communication with the mixing chamber, distribution chamber a second passage 905b communicates with the second siphon through the mixing chamber 906, mixing chamber 906 through the third siphon passage communicates with the distribution channel, the reaction chamber communication with the dispensing passage 907, when the reaction chamber 907 is two or more, any two of the reaction chamber 907 are sequentially distributed along the distribution channel, the above-described test strip chamber 908 communicates with the distribution channel. The distribution channel 907 is located above the distal end of the reaction chamber, the chamber 908 positioned above the test strip distal end 907 of the reaction chamber; said chip 900 is provided with a vent hole 911, the vent holes 911 through the first channel and the second distribution chamber 905b and a distribution passage, and a second buffer chamber 904 communicates through a second distribution chamber vent passage 905b, the buffer chamber 904 via the third breather passage communicates with the distribution channel.

[0126] When necessary the reaction chamber 907 absorbance detector or color detection, the workflow of the chip 900 is: amplification reaction reagents are previously set in a reagent chamber 903, the sample to be detected primer is previously fixed to the reaction chamber 907; after the use of pressure-sensitive surface of the chip 900 is sealed, it can be long-term storage. In use, the pressure-sensitive adhesive sample hole 901 at the tear, the sample was added using a pipette to the sample chamber 902; After the addition was complete, the injector using the pressure-sensitive hole is sealed, the chip 900 into the chip tray 800; lysed by mechanical samples within the sample chamber 400 of cleavage block 902. Then, after high-speed centrifugation, lysed sample through the distribution chamber into the first 905a, a reagent or a reagent chamber 903 through the second amplification reagent into the distribution chamber 905b; and then, after high-speed centrifugation, sample and reagent into the mixing chamber 906 by switching the high and low speed so that the mixture thoroughly mixed; then, after high-speed centrifugation, the reaction mixture was successively into the chamber 907 and the strip cavity 908, in contact with a preset primers sample in the reaction chamber 907, the reaction was complete full after detection results. At this time, detection of absorbance or color can be detected on the reaction chamber 907.

[0127] When the test strip needs to imaging detection chamber 908, reagent chambers 903 without providing a reactant, the reaction chamber 907 without providing a primer of the sample to be detected. Said chip 900 workflow is as follows: pre-test strip is fixed to the strip cavity 908; after use the pressure-sensitive surface of the chip seal 900 may be made long-term storage. In use, the pressure-sensitive adhesive sample hole 901 at the tear, the sample was added using a pipette to the sample chamber 902; After the addition was complete, the injector using the pressure-sensitive seal bore 901, the chip 900 into the chip tray 800; through high-speed centrifugation, lysed sample through into the first distribution chamber 905a; then, after high-speed centrifugation, the sample enters the mixing chamber 906; then, after high-speed centrifugation, the sample sequentially into the reaction chamber 907 and a strip chamber in 908, the sample strip in the test strip 908 in contact with the chamber, after the completion of the reaction sufficiently, the results of detection. At this time, the cavity 908 to the test strip for detection imaging.

[0128] Said chip 900 and at least two evenly distributed along the periphery of the chip tray 800. For the shape of the chip 900, according to the actual needs, such as the above chip 900 or approximately fan-shaped sector, which is not limited to this embodiment.

[0129] In order to facilitate mounting the chip 900, 800 of the chip tray is provided with a chip card slot 801, a chip 900 connected to the chip card slot 801.

[0130] Said chip 900 may be machined from a plastic material, to ensure transparency to facilitate chip 900, to facilitate detection, and ease of processing.

[0131] The present embodiment is also designed specifically for the 900 chip test strip reaction. Specifically, such as Figure 13A with Figure 13B As shown in the test strip 900 includes a chip cavity 908; and the chip 900 is provided with a detecting means, the detecting means comprises: inlet 914, located at the distal end of the inlet 914 and capable of communicating with the intake inlet 914 sample chamber 915, located in the injection chamber 915 of the distal end of the reservoir chamber and capable of communicating with the sample chamber 915 916, located in the reservoir chamber and the proximal end of test strip 916 can be in communication with the chamber reservoir chamber 916908 .

[0132] In preparing the chip 900, first test strip to be tested into the test strip chamber 908, after the entire chip using the single plastic seal 900.

[0133] Said chip 900 using the operation process: first tear single plastic injection port at 914, using a pipette to be tested is added into the liquid sample chamber 915, after use single plastic chip inlet 914 is closed, the liquid since the inlet 914 into the injection chamber 915, the chip 900 is placed on the chip tray 800, the upper cover 10 closed; high-speed rotation of the centrifuge module 300, such that the liquid to be detected is transferred from the sample chamber 915 to the reservoir chamber 916 the liquid to be detected in the immersion test strip distal end; module 300 then stops driving rotation of the liquid to be tested is drawn into the test strip under the action of capillary force; after a period of reaction, the test image detection module 600 pairs local paper is imaged, the data processing after the detection results obtained, the detection module 600 and the imaging control module 200 connected to the communication, i.e., the detection module 600 detects the imaging structure 200 back to the control module.

[0134] The shape of the die 900, is selected according to actual needs. To simplify installation, said chip select 900 is circular, the detection unit may be one, or may be two or more. In order to improve the detection efficiency, the detection unit and at least two chip 900 along the circumferential direction evenly distributed.

[0135] In order to facilitate mounting a chip 900, such as Figure 13B with Figure 13C As shown in the chip 900 is provided with a fixing groove 917, the groove 917 of the fixed side wall is provided with a positioning groove; chip tray 800 and the fixing groove 917 is provided with a snap-fit ​​clamping means 805 and a positioning member 806 is fitted with the positioning slot is positioned .

[0136] In the actual application, it is also possible to select the above chip 900 and the chip tray 800 through other structural connections, the present embodiment does not limit this.

[0137] In the above-described centrifugal microfluidic detection system, the chip 900 can also be other types and is not limited to the above embodiments.

[0138] The present invention can be implemented or used in the art of the present invention in the above description of the disclosed embodiments.A variety of modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention.Accordingly, the present invention will not be limited to the embodiments shown herein, but rather consistent with the widest range of principles and novel features disclosed herein.