Application of bacteroides in preparation of products for preventing and treating Alzheimer's disease
A technology of Bacteroides and Bacteroides multinerii, applied in the field of Bacteroides, to reduce Aβ deposition, good therapeutic and preventive effects, and inhibit the spread of Aβ to the cortex
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Embodiment 1
[0038] Embodiment 1, the cultivation of bacteroides
[0039] Bacteroides ovatus (ATCC BAA-1304), Bacteroides vulgatus (ATCC 8482) were purchased from American type culture collection (American type culture collection, ATCC), Bacteroides dorei (DSM 17855) were purchased from the German Culture Collection of Microorganisms (Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ).
[0040] The purchased strains were aseptically inoculated into liquid medium, and then cultured in an anaerobic workstation containing 5% carbon dioxide, 10% hydrogen and 85% nitrogen.
[0041] Among them, the medium formula is shown in Table 1.
[0042] Table 1 Culture medium composition of Bacteroides
[0043]
[0044]
Embodiment 2
[0045] Example 2. Effect of Bacteroides on Aβ Deposition in the Brain of Adult Male Mice
[0046] In this experiment, a kind of Alzheimer’s disease model mouse—5×FAD mouse was used. The 5×FAD mouse was purchased from the Jackson Transgenic Mouse Collection Center; the breeding temperature was 20-24°C, the ambient humidity was 50-60%, and the light was 12 Hours (8:00-20:00), soundproof, food and water ad libitum.
[0047] Six-week-old male 5×FAD transgenic mice were used, and each group of three was randomly divided into experimental group and control group. Firstly, the mice in the experimental group and the control group were treated with antibiotics for two weeks respectively. The specific composition of the antibiotics is shown in Table 2, so as to destroy the intestinal microorganisms in the experimental mice.
[0048] Table 2 Composition of antibiotics used in antibiotic treatment
[0049] components Proportion weight neomycin sulfate 0.5mg / ml Me...
Embodiment 3
[0060] Example 3, Effects of Bacteroides on Aβ Deposition in the Brain of Young Mice
[0061] In this experiment, a kind of Alzheimer’s disease model mouse—5×FAD mouse was used. The 5×FAD mouse was purchased from the Jackson Transgenic Mouse Collection Center; the breeding temperature was 20-24°C, the ambient humidity was 50-60%, and the light was 12 Hours (8:00-20:00), soundproof, food and water ad libitum.
[0062] Male 5×FAD transgenic mice were used. After weaning on the 21st day, antibiotic treatment was started on the 22nd day. The method of gavage was adopted, and the gavage volume was 0.2ml / mouse, once a day, for three consecutive days. The specific antibiotic composition was as follows: Table 2 shows. Every 3 as a group, randomly divided into experimental group and control group.
[0063] From the 25th day, the mice in the experimental group received the active bacterial agent cultured in Example 1 orally. The bacterial liquid needs to be cultivated in advance, acti...
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